Approaches about the expression of two MADS-box genes in pear during the seasonal leaf bud-dormancy transitional phases in the subtropical climate

Approaches about the expression of two MADS-box genes in pear during the seasonal leaf bud-dormancy transitional phases in the subtropical climate

ABSTRACT In the last decades, several biochemical and molecular studies in pear (Pyrus spp.) have revealed processes associated with dormancy establishment and release, leading to the identification of several MADS-box family genes related to bud dormancy regulation. Thus, the aim of this study was to evaluate a preliminary expression pattern of the PpDAM13-1 and PpDAM13-2 MADS-box genes in pear leaf buds (Pyrus pyrifolia Nakai, cultivars Hosui and Nijisseiki; and Pyrus communis L., cultivars Rocha and Santa Maria), grown in the subtropical climate of Southern Brazil. Lateral leaf buds were collected from June to September, and RNA was extracted for evaluation of gene expression by reverse transcription polymerase chain reaction (RT-qPCR). It was found that these two genes decreased the expression as leaf buds are release from dormancy. The Hosui cultivar for both genes and the Nijisseiki cultivar for PpDAM13-2 gene showed peaks of expression in early June, indicating that leaf buds are in a transition dormancy phase. The two European cultivars showed this transition dormancy phase between the end-July and the mid-August. It was concluded that the expression analysis of PpDAM13-1 and PpDAM13-2 genes can be used as a marker to evaluate changes of dormancy phase in pear trees in subtropical climate conditions, and it can be suitable to assist management strategies for overcoming pears’ bud dormancy.

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Main Authors: Leivas,Gabrielle, Klumb,Elsa Kuhn, Bianchi,Valmor João, Yamamoto,Robson Ryu, Amarante,Luciano de, Pereira,José Francisco Martins, Herter,Flávio Gilberto, Chaves,Ana Lúcia Soares, Mello-Farias,Paulo Celso de
Format: Digital revista
Language:English
Published: Instituto Agronômico de Campinas 2022
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052022000100402
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spelling oai:scielo:S0006-870520220001004022022-02-14Approaches about the expression of two MADS-box genes in pear during the seasonal leaf bud-dormancy transitional phases in the subtropical climateLeivas,GabrielleKlumb,Elsa KuhnBianchi,Valmor JoãoYamamoto,Robson RyuAmarante,Luciano dePereira,José Francisco MartinsHerter,Flávio GilbertoChaves,Ana Lúcia SoaresMello-Farias,Paulo Celso de Pyrus spp. bud dormancy warm winter RT-qPCR gene expression ABSTRACT In the last decades, several biochemical and molecular studies in pear (Pyrus spp.) have revealed processes associated with dormancy establishment and release, leading to the identification of several MADS-box family genes related to bud dormancy regulation. Thus, the aim of this study was to evaluate a preliminary expression pattern of the PpDAM13-1 and PpDAM13-2 MADS-box genes in pear leaf buds (Pyrus pyrifolia Nakai, cultivars Hosui and Nijisseiki; and Pyrus communis L., cultivars Rocha and Santa Maria), grown in the subtropical climate of Southern Brazil. Lateral leaf buds were collected from June to September, and RNA was extracted for evaluation of gene expression by reverse transcription polymerase chain reaction (RT-qPCR). It was found that these two genes decreased the expression as leaf buds are release from dormancy. The Hosui cultivar for both genes and the Nijisseiki cultivar for PpDAM13-2 gene showed peaks of expression in early June, indicating that leaf buds are in a transition dormancy phase. The two European cultivars showed this transition dormancy phase between the end-July and the mid-August. It was concluded that the expression analysis of PpDAM13-1 and PpDAM13-2 genes can be used as a marker to evaluate changes of dormancy phase in pear trees in subtropical climate conditions, and it can be suitable to assist management strategies for overcoming pears’ bud dormancy.info:eu-repo/semantics/openAccessInstituto Agronômico de CampinasBragantia v.81 20222022-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052022000100402en10.1590/1678-4499.20210211
institution SCIELO
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country Brasil
countrycode BR
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access En linea
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language English
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author Leivas,Gabrielle
Klumb,Elsa Kuhn
Bianchi,Valmor João
Yamamoto,Robson Ryu
Amarante,Luciano de
Pereira,José Francisco Martins
Herter,Flávio Gilberto
Chaves,Ana Lúcia Soares
Mello-Farias,Paulo Celso de
spellingShingle Leivas,Gabrielle
Klumb,Elsa Kuhn
Bianchi,Valmor João
Yamamoto,Robson Ryu
Amarante,Luciano de
Pereira,José Francisco Martins
Herter,Flávio Gilberto
Chaves,Ana Lúcia Soares
Mello-Farias,Paulo Celso de
Approaches about the expression of two MADS-box genes in pear during the seasonal leaf bud-dormancy transitional phases in the subtropical climate
author_facet Leivas,Gabrielle
Klumb,Elsa Kuhn
Bianchi,Valmor João
Yamamoto,Robson Ryu
Amarante,Luciano de
Pereira,José Francisco Martins
Herter,Flávio Gilberto
Chaves,Ana Lúcia Soares
Mello-Farias,Paulo Celso de
author_sort Leivas,Gabrielle
title Approaches about the expression of two MADS-box genes in pear during the seasonal leaf bud-dormancy transitional phases in the subtropical climate
title_short Approaches about the expression of two MADS-box genes in pear during the seasonal leaf bud-dormancy transitional phases in the subtropical climate
title_full Approaches about the expression of two MADS-box genes in pear during the seasonal leaf bud-dormancy transitional phases in the subtropical climate
title_fullStr Approaches about the expression of two MADS-box genes in pear during the seasonal leaf bud-dormancy transitional phases in the subtropical climate
title_full_unstemmed Approaches about the expression of two MADS-box genes in pear during the seasonal leaf bud-dormancy transitional phases in the subtropical climate
title_sort approaches about the expression of two mads-box genes in pear during the seasonal leaf bud-dormancy transitional phases in the subtropical climate
description ABSTRACT In the last decades, several biochemical and molecular studies in pear (Pyrus spp.) have revealed processes associated with dormancy establishment and release, leading to the identification of several MADS-box family genes related to bud dormancy regulation. Thus, the aim of this study was to evaluate a preliminary expression pattern of the PpDAM13-1 and PpDAM13-2 MADS-box genes in pear leaf buds (Pyrus pyrifolia Nakai, cultivars Hosui and Nijisseiki; and Pyrus communis L., cultivars Rocha and Santa Maria), grown in the subtropical climate of Southern Brazil. Lateral leaf buds were collected from June to September, and RNA was extracted for evaluation of gene expression by reverse transcription polymerase chain reaction (RT-qPCR). It was found that these two genes decreased the expression as leaf buds are release from dormancy. The Hosui cultivar for both genes and the Nijisseiki cultivar for PpDAM13-2 gene showed peaks of expression in early June, indicating that leaf buds are in a transition dormancy phase. The two European cultivars showed this transition dormancy phase between the end-July and the mid-August. It was concluded that the expression analysis of PpDAM13-1 and PpDAM13-2 genes can be used as a marker to evaluate changes of dormancy phase in pear trees in subtropical climate conditions, and it can be suitable to assist management strategies for overcoming pears’ bud dormancy.
publisher Instituto Agronômico de Campinas
publishDate 2022
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052022000100402
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