Inheritance patterns and identification of microsatellite markers linked to the rice blast resistance in BC2F1 population of rice breeding

Inheritance patterns and identification of microsatellite markers linked to the rice blast resistance in BC2F1 population of rice breeding

The BC2F1 population was derived from a cross between rice variety, MR219 (susceptible to blast) and Pongsu Seribu 1 (resistant to blast). The objectives of this research were to know the inheritance pattern of blast resistance and to identify the linked markers associated with blast resistance in BC2F1 population. Sixteen microsatellite markers were found as polymorphic between the parents related to blast resistant genes (Pi-genes). Among the selected blast resistant linked markers, two markers RM6836 and RM8225 showed expected testcross ratio (1:1) for single-gene model in the BC2F1 population with the association between resistant and susceptible progeny. A total of 333-BC2F1 plants were challenged with the most virulent pathotype P7.2 of Magnaporthe oryzae. Chi-square (χ2) analysis for phenotypic segregation in single-gene model showed goodness of fit (P = 0.4463) to the expected segregation ratio (1:1). In marker segregation analysis, two polymorphic markers (RM6836 and RM8225) clearly showed goodness of fit to the expected segregation testcross ratio (1:1) for the single-gene model. The marker RM8225 and RM6836 showed significant R2 values higher than 10 for the trait of the blast lesions degree (BLD). The positions of RM6836 and RM8225 markers on rice chromosome 6 and the distance between these two markers is 0.2 cM. We conclude that single dominant gene control the blast resistance in Pongsu Seribu 1 located on chromosome 6, which is linked to RM8225 and RM6836 microsatellite markers. This information could be useful in marker-assisted selection for blast resistance in rice breeding involving Pongsu Seribu 1.

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Main Authors: Miah,Gous, Rafii,Mohd Y., Ismail,Mohd Razi, Puteh,Adam Bin, Rahim,Harun Abdul, Ashkani,Sadegh, Latif,Abdul
Format: Digital revista
Language:English
Published: Instituto Agronômico de Campinas 2015
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052015000100033
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spelling oai:scielo:S0006-870520150001000332015-07-28Inheritance patterns and identification of microsatellite markers linked to the rice blast resistance in BC2F1 population of rice breedingMiah,GousRafii,Mohd Y.Ismail,Mohd RaziPuteh,Adam BinRahim,Harun AbdulAshkani,SadeghLatif,Abdul blast inheritance microsatellite markers BC2F1 population rice variety The BC2F1 population was derived from a cross between rice variety, MR219 (susceptible to blast) and Pongsu Seribu 1 (resistant to blast). The objectives of this research were to know the inheritance pattern of blast resistance and to identify the linked markers associated with blast resistance in BC2F1 population. Sixteen microsatellite markers were found as polymorphic between the parents related to blast resistant genes (Pi-genes). Among the selected blast resistant linked markers, two markers RM6836 and RM8225 showed expected testcross ratio (1:1) for single-gene model in the BC2F1 population with the association between resistant and susceptible progeny. A total of 333-BC2F1 plants were challenged with the most virulent pathotype P7.2 of Magnaporthe oryzae. Chi-square (χ2) analysis for phenotypic segregation in single-gene model showed goodness of fit (P = 0.4463) to the expected segregation ratio (1:1). In marker segregation analysis, two polymorphic markers (RM6836 and RM8225) clearly showed goodness of fit to the expected segregation testcross ratio (1:1) for the single-gene model. The marker RM8225 and RM6836 showed significant R2 values higher than 10 for the trait of the blast lesions degree (BLD). The positions of RM6836 and RM8225 markers on rice chromosome 6 and the distance between these two markers is 0.2 cM. We conclude that single dominant gene control the blast resistance in Pongsu Seribu 1 located on chromosome 6, which is linked to RM8225 and RM6836 microsatellite markers. This information could be useful in marker-assisted selection for blast resistance in rice breeding involving Pongsu Seribu 1.info:eu-repo/semantics/openAccessInstituto Agronômico de CampinasBragantia v.74 n.1 20152015-03-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052015000100033en10.1590/1678-4499.0311
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Miah,Gous
Rafii,Mohd Y.
Ismail,Mohd Razi
Puteh,Adam Bin
Rahim,Harun Abdul
Ashkani,Sadegh
Latif,Abdul
spellingShingle Miah,Gous
Rafii,Mohd Y.
Ismail,Mohd Razi
Puteh,Adam Bin
Rahim,Harun Abdul
Ashkani,Sadegh
Latif,Abdul
Inheritance patterns and identification of microsatellite markers linked to the rice blast resistance in BC2F1 population of rice breeding
author_facet Miah,Gous
Rafii,Mohd Y.
Ismail,Mohd Razi
Puteh,Adam Bin
Rahim,Harun Abdul
Ashkani,Sadegh
Latif,Abdul
author_sort Miah,Gous
title Inheritance patterns and identification of microsatellite markers linked to the rice blast resistance in BC2F1 population of rice breeding
title_short Inheritance patterns and identification of microsatellite markers linked to the rice blast resistance in BC2F1 population of rice breeding
title_full Inheritance patterns and identification of microsatellite markers linked to the rice blast resistance in BC2F1 population of rice breeding
title_fullStr Inheritance patterns and identification of microsatellite markers linked to the rice blast resistance in BC2F1 population of rice breeding
title_full_unstemmed Inheritance patterns and identification of microsatellite markers linked to the rice blast resistance in BC2F1 population of rice breeding
title_sort inheritance patterns and identification of microsatellite markers linked to the rice blast resistance in bc2f1 population of rice breeding
description The BC2F1 population was derived from a cross between rice variety, MR219 (susceptible to blast) and Pongsu Seribu 1 (resistant to blast). The objectives of this research were to know the inheritance pattern of blast resistance and to identify the linked markers associated with blast resistance in BC2F1 population. Sixteen microsatellite markers were found as polymorphic between the parents related to blast resistant genes (Pi-genes). Among the selected blast resistant linked markers, two markers RM6836 and RM8225 showed expected testcross ratio (1:1) for single-gene model in the BC2F1 population with the association between resistant and susceptible progeny. A total of 333-BC2F1 plants were challenged with the most virulent pathotype P7.2 of Magnaporthe oryzae. Chi-square (χ2) analysis for phenotypic segregation in single-gene model showed goodness of fit (P = 0.4463) to the expected segregation ratio (1:1). In marker segregation analysis, two polymorphic markers (RM6836 and RM8225) clearly showed goodness of fit to the expected segregation testcross ratio (1:1) for the single-gene model. The marker RM8225 and RM6836 showed significant R2 values higher than 10 for the trait of the blast lesions degree (BLD). The positions of RM6836 and RM8225 markers on rice chromosome 6 and the distance between these two markers is 0.2 cM. We conclude that single dominant gene control the blast resistance in Pongsu Seribu 1 located on chromosome 6, which is linked to RM8225 and RM6836 microsatellite markers. This information could be useful in marker-assisted selection for blast resistance in rice breeding involving Pongsu Seribu 1.
publisher Instituto Agronômico de Campinas
publishDate 2015
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052015000100033
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