Lacrimal gland primary acinar cell culture: the role of insulin
ABSTRACT Purpose: The goal of the present study was to establish a protocol for primary culture of lacrimal gland acinar cells (LGACs) and to assess the effect of adding insulin to the culture media. Methods: LGACs were isolated and cultured from lacrimal glands of Wistar male rats. The study outcomes included cell number, viability, and peroxidase release over time and in response to three concentrations of insulin (0.5, 5.0, and 50.0 μg/mL). Results: In LGAC primary culture, cells started to form clusters by day 3. There was a time-response pattern of peroxidase release, which rose by day 6, in response to carbachol. Culture viability lasted for 12 days. An insulin concentration of 5.0 μg/mL in the culture medium resulted in higher viability and secretory capacity. Conclusions: The present method simplifies the isolation and culture of LGACs. The data confirmed the relevance of adding insulin to maintain LGACs in culture.
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Digital revista |
| Language: | English |
| Published: |
Conselho Brasileiro de Oftalmologia
2016
|
| Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492016000200105 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
oai:scielo:S0004-27492016000200105 |
|---|---|
| record_format |
ojs |
| spelling |
oai:scielo:S0004-274920160002001052016-05-18Lacrimal gland primary acinar cell culture: the role of insulinMalki,Leonardo TannusDias,Ana CarolinaJorge,Angelica GobbiMódulo,Carolina MariaRocha,Eduardo Melani Acinar cells Lacrimal gland Lacrimal apparatus Insulin Peroxidase Cell Count Regenerative medicine Tissue engineering Animals Rats, Wistar ABSTRACT Purpose: The goal of the present study was to establish a protocol for primary culture of lacrimal gland acinar cells (LGACs) and to assess the effect of adding insulin to the culture media. Methods: LGACs were isolated and cultured from lacrimal glands of Wistar male rats. The study outcomes included cell number, viability, and peroxidase release over time and in response to three concentrations of insulin (0.5, 5.0, and 50.0 μg/mL). Results: In LGAC primary culture, cells started to form clusters by day 3. There was a time-response pattern of peroxidase release, which rose by day 6, in response to carbachol. Culture viability lasted for 12 days. An insulin concentration of 5.0 μg/mL in the culture medium resulted in higher viability and secretory capacity. Conclusions: The present method simplifies the isolation and culture of LGACs. The data confirmed the relevance of adding insulin to maintain LGACs in culture.info:eu-repo/semantics/openAccessConselho Brasileiro de OftalmologiaArquivos Brasileiros de Oftalmologia v.79 n.2 20162016-04-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492016000200105en10.5935/0004-2749.20160031 |
| institution |
SCIELO |
| collection |
OJS |
| country |
Brasil |
| countrycode |
BR |
| component |
Revista |
| access |
En linea |
| databasecode |
rev-scielo-br |
| tag |
revista |
| region |
America del Sur |
| libraryname |
SciELO |
| language |
English |
| format |
Digital |
| author |
Malki,Leonardo Tannus Dias,Ana Carolina Jorge,Angelica Gobbi Módulo,Carolina Maria Rocha,Eduardo Melani |
| spellingShingle |
Malki,Leonardo Tannus Dias,Ana Carolina Jorge,Angelica Gobbi Módulo,Carolina Maria Rocha,Eduardo Melani Lacrimal gland primary acinar cell culture: the role of insulin |
| author_facet |
Malki,Leonardo Tannus Dias,Ana Carolina Jorge,Angelica Gobbi Módulo,Carolina Maria Rocha,Eduardo Melani |
| author_sort |
Malki,Leonardo Tannus |
| title |
Lacrimal gland primary acinar cell culture: the role of insulin |
| title_short |
Lacrimal gland primary acinar cell culture: the role of insulin |
| title_full |
Lacrimal gland primary acinar cell culture: the role of insulin |
| title_fullStr |
Lacrimal gland primary acinar cell culture: the role of insulin |
| title_full_unstemmed |
Lacrimal gland primary acinar cell culture: the role of insulin |
| title_sort |
lacrimal gland primary acinar cell culture: the role of insulin |
| description |
ABSTRACT Purpose: The goal of the present study was to establish a protocol for primary culture of lacrimal gland acinar cells (LGACs) and to assess the effect of adding insulin to the culture media. Methods: LGACs were isolated and cultured from lacrimal glands of Wistar male rats. The study outcomes included cell number, viability, and peroxidase release over time and in response to three concentrations of insulin (0.5, 5.0, and 50.0 μg/mL). Results: In LGAC primary culture, cells started to form clusters by day 3. There was a time-response pattern of peroxidase release, which rose by day 6, in response to carbachol. Culture viability lasted for 12 days. An insulin concentration of 5.0 μg/mL in the culture medium resulted in higher viability and secretory capacity. Conclusions: The present method simplifies the isolation and culture of LGACs. The data confirmed the relevance of adding insulin to maintain LGACs in culture. |
| publisher |
Conselho Brasileiro de Oftalmologia |
| publishDate |
2016 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492016000200105 |
| work_keys_str_mv |
AT malkileonardotannus lacrimalglandprimaryacinarcellculturetheroleofinsulin AT diasanacarolina lacrimalglandprimaryacinarcellculturetheroleofinsulin AT jorgeangelicagobbi lacrimalglandprimaryacinarcellculturetheroleofinsulin AT modulocarolinamaria lacrimalglandprimaryacinarcellculturetheroleofinsulin AT rochaeduardomelani lacrimalglandprimaryacinarcellculturetheroleofinsulin |
| _version_ |
1756373217645166592 |