Enhancing T3 and cAMP responsive gene participation in the thermogenic regulation of fuel oxidation pathways
OBJECTIVE: We sought to identify glycolysis, glycogenolysis, lipolysis, Krebs cycle, respiratory chain, and oxidative phosphorylation enzymes simultaneously regulated by T3 and cAMP. MATERIALS AND METHODS: We performed in silico analysis of 56 promoters to search for cis-cAMP (CREB) and cis-thyroid (TRE) response elements, considering UCP1, SERCA2 and glyceraldehyde 3-phosphate dehydrogenase as reference. Only regulatory regions with prior in vitro validation were selected. RESULTS: 29/56 enzymes presented potential TREs in their regulatory sequence, and some scored over 0.80 (better predictive value 1): citrate synthase, phosphoglucose isomerase, succinate dehydrogenases A/C, UCP3, UCP2, UCP4, UCP5, phosphoglycerate mutase, glyceraldehyde 3-P dehydrogenase, glucokinase, malate dehydrogenase, acyl-CoA transferase (thiolase), cytochrome a3, and lactate dehydrogenase. Moreover, some enzymes have not yet been described in the literature as genomically regulated by T3. CONCLUSION: Our results point to other enzymes which may possibly be regulated by T3 and CREB, and speculate their joint roles in contributing to the optimal thermogenic acclimation.
Main Authors: | Dorsa,Karina Kores, Santos,Michelle Venâncio dos, Silva,Magnus R. Dias da |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira de Endocrinologia e Metabologia
2010
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27302010000400007 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Thermogenic flowering of taro (Colocasia esculenta, Araceae)
by: Ivancic, Anton, et al. -
Thermogenic flowering of the giant taro (Alocasia macrorrhizos, Araceae)
by: Ivancic, Anton, et al. -
Topology of thermogenic tissues of Alocasia macrorrhizos (Araceae) inflorescences
by: Ivancic, Anton, et al. -
Evidence for a modulation of neutral trehalase activity by Ca2+ and cAMP signaling pathways in Saccharomyces cerevisiae
by: Souza,A.C., et al.
Published: (2002) -
Depression promotes prostate cancer invasion and metastasis via a sympathetic-cAMP-FAK signaling pathway
by: Cheng, Yan, et al.
Published: (2018)