Reduction of the DNA damages, Hepatoprotective Effect and Antioxidant Potential of the Coconut Water, ascorbic and Caffeic Acids in Oxidative Stress Mediated by Ethanol

Reduction of the DNA damages, Hepatoprotective Effect and Antioxidant Potential of the Coconut Water, ascorbic and Caffeic Acids in Oxidative Stress Mediated by Ethanol

ABSTRACT Hepatic disorders such as steatosis and alcoholic steatohepatitis are common diseases that affect thousands of people around the globe. This study aims to identify the main phenol compounds using a new HPLC-ESI+-MS/MS method, to evaluate some oxidative stress parameters and the hepatoprotective action of green dwarf coconut water, caffeic and ascorbic acids on the liver and serum of rats treated with ethanol. The results showed five polyphenols in the lyophilized coconut water spiked with standards: chlorogenic acid (0.18 µM), caffeic acid (1.1 µM), methyl caffeate (0.03 µM), quercetin (0.08 µM) and ferulic acid (0.02 µM) isomers. In the animals, the activity of the serum γ-glutamyltranspeptidase (γ-GT) was reduced to 1.8 I.U/L in the coconut water group, 3.6 I.U/L in the ascorbic acid group and 2.9 I.U/L in the caffeic acid groups, when compared with the ethanol group (5.1 I.U/L, p<0.05). Still in liver, the DNA analysis demonstrated a decrease of oxidized bases compared to ethanol group of 36.2% and 48.0% for pretreated and post treated coconut water group respectively, 42.5% for the caffeic acid group, and 34.5% for the ascorbic acid group. The ascorbic acid was efficient in inhibiting the thiobarbituric acid reactive substances (TBARS) in the liver by 16.5% in comparison with the ethanol group. These data indicate that the green dwarf coconut water, caffeic and ascorbic acids have antioxidant, hepatoprotective and reduced DNA damage properties, thus decreasing the oxidative stress induced by ethanol metabolism.

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Main Authors: BISPO,VANDERSON S., DANTAS,LUCAS S., CHAVES FILHO,ADRIANO B., PINTO,ISABELLA F.D., SILVA,RAILMARA P. DA, OTSUKA,FELIPE A.M., SANTOS,RODRIGO B., SANTOS,ALINE C., TRINDADE,DANIELLE J., MATOS,HUMBERTO R.
Format: Digital revista
Language:English
Published: Academia Brasileira de Ciências 2017
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301095
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spelling oai:scielo:S0001-376520170003010952017-06-07Reduction of the DNA damages, Hepatoprotective Effect and Antioxidant Potential of the Coconut Water, ascorbic and Caffeic Acids in Oxidative Stress Mediated by EthanolBISPO,VANDERSON S.DANTAS,LUCAS S.CHAVES FILHO,ADRIANO B.PINTO,ISABELLA F.D.SILVA,RAILMARA P. DAOTSUKA,FELIPE A.M.SANTOS,RODRIGO B.SANTOS,ALINE C.TRINDADE,DANIELLE J.MATOS,HUMBERTO R. Coconut water ethanol oxidative stress phenolic compounds ABSTRACT Hepatic disorders such as steatosis and alcoholic steatohepatitis are common diseases that affect thousands of people around the globe. This study aims to identify the main phenol compounds using a new HPLC-ESI+-MS/MS method, to evaluate some oxidative stress parameters and the hepatoprotective action of green dwarf coconut water, caffeic and ascorbic acids on the liver and serum of rats treated with ethanol. The results showed five polyphenols in the lyophilized coconut water spiked with standards: chlorogenic acid (0.18 µM), caffeic acid (1.1 µM), methyl caffeate (0.03 µM), quercetin (0.08 µM) and ferulic acid (0.02 µM) isomers. In the animals, the activity of the serum γ-glutamyltranspeptidase (γ-GT) was reduced to 1.8 I.U/L in the coconut water group, 3.6 I.U/L in the ascorbic acid group and 2.9 I.U/L in the caffeic acid groups, when compared with the ethanol group (5.1 I.U/L, p<0.05). Still in liver, the DNA analysis demonstrated a decrease of oxidized bases compared to ethanol group of 36.2% and 48.0% for pretreated and post treated coconut water group respectively, 42.5% for the caffeic acid group, and 34.5% for the ascorbic acid group. The ascorbic acid was efficient in inhibiting the thiobarbituric acid reactive substances (TBARS) in the liver by 16.5% in comparison with the ethanol group. These data indicate that the green dwarf coconut water, caffeic and ascorbic acids have antioxidant, hepatoprotective and reduced DNA damage properties, thus decreasing the oxidative stress induced by ethanol metabolism.info:eu-repo/semantics/openAccessAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências v.89 n.2 20172017-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301095en10.1590/0001-3765201720160581
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region America del Sur
libraryname SciELO
language English
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author BISPO,VANDERSON S.
DANTAS,LUCAS S.
CHAVES FILHO,ADRIANO B.
PINTO,ISABELLA F.D.
SILVA,RAILMARA P. DA
OTSUKA,FELIPE A.M.
SANTOS,RODRIGO B.
SANTOS,ALINE C.
TRINDADE,DANIELLE J.
MATOS,HUMBERTO R.
spellingShingle BISPO,VANDERSON S.
DANTAS,LUCAS S.
CHAVES FILHO,ADRIANO B.
PINTO,ISABELLA F.D.
SILVA,RAILMARA P. DA
OTSUKA,FELIPE A.M.
SANTOS,RODRIGO B.
SANTOS,ALINE C.
TRINDADE,DANIELLE J.
MATOS,HUMBERTO R.
Reduction of the DNA damages, Hepatoprotective Effect and Antioxidant Potential of the Coconut Water, ascorbic and Caffeic Acids in Oxidative Stress Mediated by Ethanol
author_facet BISPO,VANDERSON S.
DANTAS,LUCAS S.
CHAVES FILHO,ADRIANO B.
PINTO,ISABELLA F.D.
SILVA,RAILMARA P. DA
OTSUKA,FELIPE A.M.
SANTOS,RODRIGO B.
SANTOS,ALINE C.
TRINDADE,DANIELLE J.
MATOS,HUMBERTO R.
author_sort BISPO,VANDERSON S.
title Reduction of the DNA damages, Hepatoprotective Effect and Antioxidant Potential of the Coconut Water, ascorbic and Caffeic Acids in Oxidative Stress Mediated by Ethanol
title_short Reduction of the DNA damages, Hepatoprotective Effect and Antioxidant Potential of the Coconut Water, ascorbic and Caffeic Acids in Oxidative Stress Mediated by Ethanol
title_full Reduction of the DNA damages, Hepatoprotective Effect and Antioxidant Potential of the Coconut Water, ascorbic and Caffeic Acids in Oxidative Stress Mediated by Ethanol
title_fullStr Reduction of the DNA damages, Hepatoprotective Effect and Antioxidant Potential of the Coconut Water, ascorbic and Caffeic Acids in Oxidative Stress Mediated by Ethanol
title_full_unstemmed Reduction of the DNA damages, Hepatoprotective Effect and Antioxidant Potential of the Coconut Water, ascorbic and Caffeic Acids in Oxidative Stress Mediated by Ethanol
title_sort reduction of the dna damages, hepatoprotective effect and antioxidant potential of the coconut water, ascorbic and caffeic acids in oxidative stress mediated by ethanol
description ABSTRACT Hepatic disorders such as steatosis and alcoholic steatohepatitis are common diseases that affect thousands of people around the globe. This study aims to identify the main phenol compounds using a new HPLC-ESI+-MS/MS method, to evaluate some oxidative stress parameters and the hepatoprotective action of green dwarf coconut water, caffeic and ascorbic acids on the liver and serum of rats treated with ethanol. The results showed five polyphenols in the lyophilized coconut water spiked with standards: chlorogenic acid (0.18 µM), caffeic acid (1.1 µM), methyl caffeate (0.03 µM), quercetin (0.08 µM) and ferulic acid (0.02 µM) isomers. In the animals, the activity of the serum γ-glutamyltranspeptidase (γ-GT) was reduced to 1.8 I.U/L in the coconut water group, 3.6 I.U/L in the ascorbic acid group and 2.9 I.U/L in the caffeic acid groups, when compared with the ethanol group (5.1 I.U/L, p<0.05). Still in liver, the DNA analysis demonstrated a decrease of oxidized bases compared to ethanol group of 36.2% and 48.0% for pretreated and post treated coconut water group respectively, 42.5% for the caffeic acid group, and 34.5% for the ascorbic acid group. The ascorbic acid was efficient in inhibiting the thiobarbituric acid reactive substances (TBARS) in the liver by 16.5% in comparison with the ethanol group. These data indicate that the green dwarf coconut water, caffeic and ascorbic acids have antioxidant, hepatoprotective and reduced DNA damage properties, thus decreasing the oxidative stress induced by ethanol metabolism.
publisher Academia Brasileira de Ciências
publishDate 2017
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301095
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