Use of GC/MS to identify chemical constituents and cytotoxic activity of the leaves of Phoradendron mucronatum and Phoradendron microphyllum (Viscaceae)

BASTOS,ISLA V.G.A.; OLIVEIRA,TATIANE B. DE; RODRIGUES,MARIA D.; MILITÃO,GARDÊNIA C.G.; SILVA,TERESINHA G. DA; TURATTI,IZABEL C.C.; LOPES,NORBERTO P.; MELO,SEBASTIÃO J. DE

Use of GC/MS to identify chemical constituents and cytotoxic activity of the leaves of Phoradendron mucronatum and Phoradendron microphyllum (Viscaceae)

ABSTRACT Phoradendron mucronatum and P. microphyllum are plants that found in tropical and subtropical areas, used in traditional medicine and popularly known as mistle-thrush. The aim of this study was to identify the chemical constituents of different leaf extracts from P. mucronatum and P. microphyllum and assess cytotoxic activity against strains from a human tumour cells. Extracts obtained with hexane, dichloromethane, chloroform and ethyl acetate from the leaves were analysed by gas chromatography coupled with mass spectrometry (GC-MS) and the cytotoxicity was assessed by the MTT method (bromide (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)). The tested human tumour cells were NCI-H292 (human pulmonar mucoepidermoid carcinoma), MCF-7 (human breast adenocarcinoma) and HEp-2 (epidermoid carcinoma of the larynx). Analysis by GC/MS of the extracts from leaves of P. microphyllum and P. mucronatum detected 51 different compounds, such as alkaloids, diterpenes, triterpenes, sterols, alcohols, aldehydes, fatty acids and hydrocarbons. In the cytotoxic evaluation, hexane and ethyl acetate extracts from the leaves P. microphyllum inhibited cell growth of NCI-H292 strains (72.97%) and HEp-2 (87.53%), respectively. The extracts of P. mucronatum species showed an inhibitory effect towards NCI-H292 (83.19%/hexane), MCF-7 (88.69%/dichloromethane) and HEp-2 (93.40%/hexane). The extracts showed cytotoxic activity against the tested strains, especially the P. mucronatum, which presented the highest percentages of inhibition of cell growth.

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Main Authors:	BASTOS,ISLA V.G.A., OLIVEIRA,TATIANE B. DE, RODRIGUES,MARIA D., MILITÃO,GARDÊNIA C.G., SILVA,TERESINHA G. DA, TURATTI,IZABEL C.C., LOPES,NORBERTO P., MELO,SEBASTIÃO J. DE
Format:	Digital revista
Language:	English
Published:	Academia Brasileira de Ciências 2017
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000300991
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spelling	oai:scielo:S0001-376520170003009912017-06-07Use of GC/MS to identify chemical constituents and cytotoxic activity of the leaves of Phoradendron mucronatum and Phoradendron microphyllum (Viscaceae)BASTOS,ISLA V.G.A.OLIVEIRA,TATIANE B. DERODRIGUES,MARIA D.MILITÃO,GARDÊNIA C.G.SILVA,TERESINHA G. DATURATTI,IZABEL C.C.LOPES,NORBERTO P.MELO,SEBASTIÃO J. DE Cytotoxicity Gas Chromatography Phoradendron microphyllum Phoradendron mucronatum ABSTRACT Phoradendron mucronatum and P. microphyllum are plants that found in tropical and subtropical areas, used in traditional medicine and popularly known as mistle-thrush. The aim of this study was to identify the chemical constituents of different leaf extracts from P. mucronatum and P. microphyllum and assess cytotoxic activity against strains from a human tumour cells. Extracts obtained with hexane, dichloromethane, chloroform and ethyl acetate from the leaves were analysed by gas chromatography coupled with mass spectrometry (GC-MS) and the cytotoxicity was assessed by the MTT method (bromide (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)). The tested human tumour cells were NCI-H292 (human pulmonar mucoepidermoid carcinoma), MCF-7 (human breast adenocarcinoma) and HEp-2 (epidermoid carcinoma of the larynx). Analysis by GC/MS of the extracts from leaves of P. microphyllum and P. mucronatum detected 51 different compounds, such as alkaloids, diterpenes, triterpenes, sterols, alcohols, aldehydes, fatty acids and hydrocarbons. In the cytotoxic evaluation, hexane and ethyl acetate extracts from the leaves P. microphyllum inhibited cell growth of NCI-H292 strains (72.97%) and HEp-2 (87.53%), respectively. The extracts of P. mucronatum species showed an inhibitory effect towards NCI-H292 (83.19%/hexane), MCF-7 (88.69%/dichloromethane) and HEp-2 (93.40%/hexane). The extracts showed cytotoxic activity against the tested strains, especially the P. mucronatum, which presented the highest percentages of inhibition of cell growth.info:eu-repo/semantics/openAccessAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências v.89 n.2 20172017-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000300991en10.1590/0001-3765201720160586
institution	SCIELO
collection	OJS
country	Brasil
countrycode	BR
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region	America del Sur
libraryname	SciELO
language	English
format	Digital
author	BASTOS,ISLA V.G.A. OLIVEIRA,TATIANE B. DE RODRIGUES,MARIA D. MILITÃO,GARDÊNIA C.G. SILVA,TERESINHA G. DA TURATTI,IZABEL C.C. LOPES,NORBERTO P. MELO,SEBASTIÃO J. DE
spellingShingle	BASTOS,ISLA V.G.A. OLIVEIRA,TATIANE B. DE RODRIGUES,MARIA D. MILITÃO,GARDÊNIA C.G. SILVA,TERESINHA G. DA TURATTI,IZABEL C.C. LOPES,NORBERTO P. MELO,SEBASTIÃO J. DE Use of GC/MS to identify chemical constituents and cytotoxic activity of the leaves of Phoradendron mucronatum and Phoradendron microphyllum (Viscaceae)
author_facet	BASTOS,ISLA V.G.A. OLIVEIRA,TATIANE B. DE RODRIGUES,MARIA D. MILITÃO,GARDÊNIA C.G. SILVA,TERESINHA G. DA TURATTI,IZABEL C.C. LOPES,NORBERTO P. MELO,SEBASTIÃO J. DE
author_sort	BASTOS,ISLA V.G.A.
title	Use of GC/MS to identify chemical constituents and cytotoxic activity of the leaves of Phoradendron mucronatum and Phoradendron microphyllum (Viscaceae)
title_short	Use of GC/MS to identify chemical constituents and cytotoxic activity of the leaves of Phoradendron mucronatum and Phoradendron microphyllum (Viscaceae)
title_full	Use of GC/MS to identify chemical constituents and cytotoxic activity of the leaves of Phoradendron mucronatum and Phoradendron microphyllum (Viscaceae)
title_fullStr	Use of GC/MS to identify chemical constituents and cytotoxic activity of the leaves of Phoradendron mucronatum and Phoradendron microphyllum (Viscaceae)
title_full_unstemmed	Use of GC/MS to identify chemical constituents and cytotoxic activity of the leaves of Phoradendron mucronatum and Phoradendron microphyllum (Viscaceae)
title_sort	use of gc/ms to identify chemical constituents and cytotoxic activity of the leaves of phoradendron mucronatum and phoradendron microphyllum (viscaceae)
description	ABSTRACT Phoradendron mucronatum and P. microphyllum are plants that found in tropical and subtropical areas, used in traditional medicine and popularly known as mistle-thrush. The aim of this study was to identify the chemical constituents of different leaf extracts from P. mucronatum and P. microphyllum and assess cytotoxic activity against strains from a human tumour cells. Extracts obtained with hexane, dichloromethane, chloroform and ethyl acetate from the leaves were analysed by gas chromatography coupled with mass spectrometry (GC-MS) and the cytotoxicity was assessed by the MTT method (bromide (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)). The tested human tumour cells were NCI-H292 (human pulmonar mucoepidermoid carcinoma), MCF-7 (human breast adenocarcinoma) and HEp-2 (epidermoid carcinoma of the larynx). Analysis by GC/MS of the extracts from leaves of P. microphyllum and P. mucronatum detected 51 different compounds, such as alkaloids, diterpenes, triterpenes, sterols, alcohols, aldehydes, fatty acids and hydrocarbons. In the cytotoxic evaluation, hexane and ethyl acetate extracts from the leaves P. microphyllum inhibited cell growth of NCI-H292 strains (72.97%) and HEp-2 (87.53%), respectively. The extracts of P. mucronatum species showed an inhibitory effect towards NCI-H292 (83.19%/hexane), MCF-7 (88.69%/dichloromethane) and HEp-2 (93.40%/hexane). The extracts showed cytotoxic activity against the tested strains, especially the P. mucronatum, which presented the highest percentages of inhibition of cell growth.
publisher	Academia Brasileira de Ciências
publishDate	2017
url	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000300991
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Use of GC/MS to identify chemical constituents and cytotoxic activity of the leaves of Phoradendron mucronatum and Phoradendron microphyllum (Viscaceae)

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