Chemical, enzymatic and cellular antioxidant activity studies of Agaricus blazei Murrill
Mushrooms possess nutritional and medicinal properties that have long been used for human health preservation and that have been considered by researchers as possible sources of free radical scavengers. In this work, the antioxidant properties of water extracts from Agaricus blazei Murill, produced by maceration and decoction, are demonstrated in vitro. Resistance to oxidation is demonstrated through three mechanisms: i) inhibition of enzymatic oxidative process, with 100% inhibition of HRP (horseradish peroxidase) and MPO (myeloperoxidase); ii) inhibition of cellular oxidative stress, with 80% inhibition of the oxidative burst of polymorphonuclear neutrophils (PMNs); and iii) direct action over reactive species, with 62% and 87% suppression of HOCl and superoxide anion radical (O2 ), respectively. From the data, it was concluded that the aqueous extract of A. blazei has significant antioxidant activity, indicating its possible application for nutraceutical and medicinal purposes.
Main Authors: | , , , , , |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Academia Brasileira de Ciências
2013
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000301073 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:scielo:S0001-37652013000301073 |
---|---|
record_format |
ojs |
spelling |
oai:scielo:S0001-376520130003010732015-10-26Chemical, enzymatic and cellular antioxidant activity studies of Agaricus blazei MurrillHAKIME-SILVA,RICARDO A.VELLOSA,JOSÉ C.R.KHALIL,NAJEH M.KHALIL,OMAR A.K.BRUNETTI,IGUATEMY L.OLIVEIRA,OLGA M.M.F. Agaricus blazei Murrill antioxidant oxidant species chemiluminescence polymorphonuclear neutrophils Mushrooms possess nutritional and medicinal properties that have long been used for human health preservation and that have been considered by researchers as possible sources of free radical scavengers. In this work, the antioxidant properties of water extracts from Agaricus blazei Murill, produced by maceration and decoction, are demonstrated in vitro. Resistance to oxidation is demonstrated through three mechanisms: i) inhibition of enzymatic oxidative process, with 100% inhibition of HRP (horseradish peroxidase) and MPO (myeloperoxidase); ii) inhibition of cellular oxidative stress, with 80% inhibition of the oxidative burst of polymorphonuclear neutrophils (PMNs); and iii) direct action over reactive species, with 62% and 87% suppression of HOCl and superoxide anion radical (O2 ), respectively. From the data, it was concluded that the aqueous extract of A. blazei has significant antioxidant activity, indicating its possible application for nutraceutical and medicinal purposes.info:eu-repo/semantics/openAccessAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências v.85 n.3 20132013-09-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000301073en10.1590/S0001-37652013005000044 |
institution |
SCIELO |
collection |
OJS |
country |
Brasil |
countrycode |
BR |
component |
Revista |
access |
En linea |
databasecode |
rev-scielo-br |
tag |
revista |
region |
America del Sur |
libraryname |
SciELO |
language |
English |
format |
Digital |
author |
HAKIME-SILVA,RICARDO A. VELLOSA,JOSÉ C.R. KHALIL,NAJEH M. KHALIL,OMAR A.K. BRUNETTI,IGUATEMY L. OLIVEIRA,OLGA M.M.F. |
spellingShingle |
HAKIME-SILVA,RICARDO A. VELLOSA,JOSÉ C.R. KHALIL,NAJEH M. KHALIL,OMAR A.K. BRUNETTI,IGUATEMY L. OLIVEIRA,OLGA M.M.F. Chemical, enzymatic and cellular antioxidant activity studies of Agaricus blazei Murrill |
author_facet |
HAKIME-SILVA,RICARDO A. VELLOSA,JOSÉ C.R. KHALIL,NAJEH M. KHALIL,OMAR A.K. BRUNETTI,IGUATEMY L. OLIVEIRA,OLGA M.M.F. |
author_sort |
HAKIME-SILVA,RICARDO A. |
title |
Chemical, enzymatic and cellular antioxidant activity studies of Agaricus blazei Murrill |
title_short |
Chemical, enzymatic and cellular antioxidant activity studies of Agaricus blazei Murrill |
title_full |
Chemical, enzymatic and cellular antioxidant activity studies of Agaricus blazei Murrill |
title_fullStr |
Chemical, enzymatic and cellular antioxidant activity studies of Agaricus blazei Murrill |
title_full_unstemmed |
Chemical, enzymatic and cellular antioxidant activity studies of Agaricus blazei Murrill |
title_sort |
chemical, enzymatic and cellular antioxidant activity studies of agaricus blazei murrill |
description |
Mushrooms possess nutritional and medicinal properties that have long been used for human health preservation and that have been considered by researchers as possible sources of free radical scavengers. In this work, the antioxidant properties of water extracts from Agaricus blazei Murill, produced by maceration and decoction, are demonstrated in vitro. Resistance to oxidation is demonstrated through three mechanisms: i) inhibition of enzymatic oxidative process, with 100% inhibition of HRP (horseradish peroxidase) and MPO (myeloperoxidase); ii) inhibition of cellular oxidative stress, with 80% inhibition of the oxidative burst of polymorphonuclear neutrophils (PMNs); and iii) direct action over reactive species, with 62% and 87% suppression of HOCl and superoxide anion radical (O2 ), respectively. From the data, it was concluded that the aqueous extract of A. blazei has significant antioxidant activity, indicating its possible application for nutraceutical and medicinal purposes. |
publisher |
Academia Brasileira de Ciências |
publishDate |
2013 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000301073 |
work_keys_str_mv |
AT hakimesilvaricardoa chemicalenzymaticandcellularantioxidantactivitystudiesofagaricusblazeimurrill AT vellosajosecr chemicalenzymaticandcellularantioxidantactivitystudiesofagaricusblazeimurrill AT khalilnajehm chemicalenzymaticandcellularantioxidantactivitystudiesofagaricusblazeimurrill AT khalilomarak chemicalenzymaticandcellularantioxidantactivitystudiesofagaricusblazeimurrill AT brunettiiguatemyl chemicalenzymaticandcellularantioxidantactivitystudiesofagaricusblazeimurrill AT oliveiraolgammf chemicalenzymaticandcellularantioxidantactivitystudiesofagaricusblazeimurrill |
_version_ |
1756371973471993856 |