Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain
The genus Leuconostoc belongs to a group of lactic acid bacteria usually isolated from fermented vegetables, which includes species involved in the production of exopolysaccharides (EPS). These biopolymers possess considerable commercial potential. Because of the wide variety of industrial applications of EPS, this study aimed to produce and characterize the native exopolysaccharide strain Leuconostoc pseudomesenteroides R2, which was isolated from cabbage collected in a semi-arid region of Bahia. We employed the following conditions for the production of EPS: 10.7% sucrose, pH 8.2, without agitation and incubation at 28ºC for 30 hours. The fermentation broth was treated with ethanol and generated two types of polysaccharide substances (EPS I and EPS II). The identification of EPS I and EPS II was conducted using FT-IR, ¹H, 13C and DEPT-135 NMR spectra. The two substances were identified as linear dextran α polysaccharides (1 → 6) which indicated different characteristics with respect to thermal analysis and density of free packaging, viscosity and time of solubilization. Both dextrans are of low density, possess high thermal stability and exhibited the behavior characteristic of pseudoplastic polymers.
Main Authors: | , , , , , , , |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Academia Brasileira de Ciências
2012
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200018 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:scielo:S0001-37652012000200018 |
---|---|
record_format |
ojs |
spelling |
oai:scielo:S0001-376520120002000182012-05-25Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strainPaulo,Elinalva M.Boffo,Elisangela F.Branco,AlexsandroValente,Ângela M.M.P.Melo,Itamar S.Ferreira,Antonio G.Roque,Milton R.A.Assis,Sandra A. de Leuconostoc pseudomesenteroides exopolysaccharide polymer dextran The genus Leuconostoc belongs to a group of lactic acid bacteria usually isolated from fermented vegetables, which includes species involved in the production of exopolysaccharides (EPS). These biopolymers possess considerable commercial potential. Because of the wide variety of industrial applications of EPS, this study aimed to produce and characterize the native exopolysaccharide strain Leuconostoc pseudomesenteroides R2, which was isolated from cabbage collected in a semi-arid region of Bahia. We employed the following conditions for the production of EPS: 10.7% sucrose, pH 8.2, without agitation and incubation at 28ºC for 30 hours. The fermentation broth was treated with ethanol and generated two types of polysaccharide substances (EPS I and EPS II). The identification of EPS I and EPS II was conducted using FT-IR, ¹H, 13C and DEPT-135 NMR spectra. The two substances were identified as linear dextran α polysaccharides (1 → 6) which indicated different characteristics with respect to thermal analysis and density of free packaging, viscosity and time of solubilization. Both dextrans are of low density, possess high thermal stability and exhibited the behavior characteristic of pseudoplastic polymers.info:eu-repo/semantics/openAccessAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências v.84 n.2 20122012-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200018en10.1590/S0001-37652012000200018 |
institution |
SCIELO |
collection |
OJS |
country |
Brasil |
countrycode |
BR |
component |
Revista |
access |
En linea |
databasecode |
rev-scielo-br |
tag |
revista |
region |
America del Sur |
libraryname |
SciELO |
language |
English |
format |
Digital |
author |
Paulo,Elinalva M. Boffo,Elisangela F. Branco,Alexsandro Valente,Ângela M.M.P. Melo,Itamar S. Ferreira,Antonio G. Roque,Milton R.A. Assis,Sandra A. de |
spellingShingle |
Paulo,Elinalva M. Boffo,Elisangela F. Branco,Alexsandro Valente,Ângela M.M.P. Melo,Itamar S. Ferreira,Antonio G. Roque,Milton R.A. Assis,Sandra A. de Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain |
author_facet |
Paulo,Elinalva M. Boffo,Elisangela F. Branco,Alexsandro Valente,Ângela M.M.P. Melo,Itamar S. Ferreira,Antonio G. Roque,Milton R.A. Assis,Sandra A. de |
author_sort |
Paulo,Elinalva M. |
title |
Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain |
title_short |
Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain |
title_full |
Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain |
title_fullStr |
Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain |
title_full_unstemmed |
Production, extraction and characterization of exopolysaccharides produced by the native Leuconostoc pseudomesenteroides R2 strain |
title_sort |
production, extraction and characterization of exopolysaccharides produced by the native leuconostoc pseudomesenteroides r2 strain |
description |
The genus Leuconostoc belongs to a group of lactic acid bacteria usually isolated from fermented vegetables, which includes species involved in the production of exopolysaccharides (EPS). These biopolymers possess considerable commercial potential. Because of the wide variety of industrial applications of EPS, this study aimed to produce and characterize the native exopolysaccharide strain Leuconostoc pseudomesenteroides R2, which was isolated from cabbage collected in a semi-arid region of Bahia. We employed the following conditions for the production of EPS: 10.7% sucrose, pH 8.2, without agitation and incubation at 28ºC for 30 hours. The fermentation broth was treated with ethanol and generated two types of polysaccharide substances (EPS I and EPS II). The identification of EPS I and EPS II was conducted using FT-IR, ¹H, 13C and DEPT-135 NMR spectra. The two substances were identified as linear dextran α polysaccharides (1 → 6) which indicated different characteristics with respect to thermal analysis and density of free packaging, viscosity and time of solubilization. Both dextrans are of low density, possess high thermal stability and exhibited the behavior characteristic of pseudoplastic polymers. |
publisher |
Academia Brasileira de Ciências |
publishDate |
2012 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200018 |
work_keys_str_mv |
AT pauloelinalvam productionextractionandcharacterizationofexopolysaccharidesproducedbythenativeleuconostocpseudomesenteroidesr2strain AT boffoelisangelaf productionextractionandcharacterizationofexopolysaccharidesproducedbythenativeleuconostocpseudomesenteroidesr2strain AT brancoalexsandro productionextractionandcharacterizationofexopolysaccharidesproducedbythenativeleuconostocpseudomesenteroidesr2strain AT valenteangelammp productionextractionandcharacterizationofexopolysaccharidesproducedbythenativeleuconostocpseudomesenteroidesr2strain AT meloitamars productionextractionandcharacterizationofexopolysaccharidesproducedbythenativeleuconostocpseudomesenteroidesr2strain AT ferreiraantoniog productionextractionandcharacterizationofexopolysaccharidesproducedbythenativeleuconostocpseudomesenteroidesr2strain AT roquemiltonra productionextractionandcharacterizationofexopolysaccharidesproducedbythenativeleuconostocpseudomesenteroidesr2strain AT assissandraade productionextractionandcharacterizationofexopolysaccharidesproducedbythenativeleuconostocpseudomesenteroidesr2strain |
_version_ |
1756371941010178048 |