Production of extracellular lipase by the phytopathogenic fungus Fusarium solani FS1

Production of extracellular lipase by the phytopathogenic fungus Fusarium solani FS1

A Brazilian strain of Fusarium solani was tested for extracellular lipase production in peptone-olive oil medium. The fungus produced 10,500 U.l-1 of lipase after 72 hours of cultivation at 25oC in shake-flask at 120 rpm in a medium containing 3% (w/v) peptone plus 0.5% (v/v) olive oil. Glucose (1% w/v) was found to inhibit the inductive effect of olive oil. Peptone concentrations below 3% (w/v) resulted in a reduced lipase production while increased olive oil concentration (above 0.5%) did not further stimulate lipase production. The optimum lipase activity was achieved at pH 8.6 and 30oC and a good enzyme stability (80% activity retention) was observed at pH ranging from 7.6 to 8.6, and the activity rapidly dropped at temperatures above 50oC. Lipase activity was stimulated by the addition of n-hexane to the culture medium supernatants, in contrast to incubation with water-soluble solvents.

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Main Authors: Maia,Maria de Mascena Diniz, Morais,Marcia Maria Camargo de, Morais Jr.,Marcos Antonio de, Melo,Eduardo Henrique Magalhães, Lima Filho,José Luiz de
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Microbiologia 1999
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400003
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spelling oai:scielo:S0001-371419990004000032000-04-19Production of extracellular lipase by the phytopathogenic fungus Fusarium solani FS1Maia,Maria de Mascena DinizMorais,Marcia Maria Camargo deMorais Jr.,Marcos Antonio deMelo,Eduardo Henrique MagalhãesLima Filho,José Luiz de Enzyme kinetics ester hydrolysis extracellular lipase Fusarium solani A Brazilian strain of Fusarium solani was tested for extracellular lipase production in peptone-olive oil medium. The fungus produced 10,500 U.l-1 of lipase after 72 hours of cultivation at 25oC in shake-flask at 120 rpm in a medium containing 3% (w/v) peptone plus 0.5% (v/v) olive oil. Glucose (1% w/v) was found to inhibit the inductive effect of olive oil. Peptone concentrations below 3% (w/v) resulted in a reduced lipase production while increased olive oil concentration (above 0.5%) did not further stimulate lipase production. The optimum lipase activity was achieved at pH 8.6 and 30oC and a good enzyme stability (80% activity retention) was observed at pH ranging from 7.6 to 8.6, and the activity rapidly dropped at temperatures above 50oC. Lipase activity was stimulated by the addition of n-hexane to the culture medium supernatants, in contrast to incubation with water-soluble solvents.info:eu-repo/semantics/openAccessSociedade Brasileira de MicrobiologiaRevista de Microbiologia v.30 n.4 19991999-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400003en10.1590/S0001-37141999000400003
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Maia,Maria de Mascena Diniz
Morais,Marcia Maria Camargo de
Morais Jr.,Marcos Antonio de
Melo,Eduardo Henrique Magalhães
Lima Filho,José Luiz de
spellingShingle Maia,Maria de Mascena Diniz
Morais,Marcia Maria Camargo de
Morais Jr.,Marcos Antonio de
Melo,Eduardo Henrique Magalhães
Lima Filho,José Luiz de
Production of extracellular lipase by the phytopathogenic fungus Fusarium solani FS1
author_facet Maia,Maria de Mascena Diniz
Morais,Marcia Maria Camargo de
Morais Jr.,Marcos Antonio de
Melo,Eduardo Henrique Magalhães
Lima Filho,José Luiz de
author_sort Maia,Maria de Mascena Diniz
title Production of extracellular lipase by the phytopathogenic fungus Fusarium solani FS1
title_short Production of extracellular lipase by the phytopathogenic fungus Fusarium solani FS1
title_full Production of extracellular lipase by the phytopathogenic fungus Fusarium solani FS1
title_fullStr Production of extracellular lipase by the phytopathogenic fungus Fusarium solani FS1
title_full_unstemmed Production of extracellular lipase by the phytopathogenic fungus Fusarium solani FS1
title_sort production of extracellular lipase by the phytopathogenic fungus fusarium solani fs1
description A Brazilian strain of Fusarium solani was tested for extracellular lipase production in peptone-olive oil medium. The fungus produced 10,500 U.l-1 of lipase after 72 hours of cultivation at 25oC in shake-flask at 120 rpm in a medium containing 3% (w/v) peptone plus 0.5% (v/v) olive oil. Glucose (1% w/v) was found to inhibit the inductive effect of olive oil. Peptone concentrations below 3% (w/v) resulted in a reduced lipase production while increased olive oil concentration (above 0.5%) did not further stimulate lipase production. The optimum lipase activity was achieved at pH 8.6 and 30oC and a good enzyme stability (80% activity retention) was observed at pH ranging from 7.6 to 8.6, and the activity rapidly dropped at temperatures above 50oC. Lipase activity was stimulated by the addition of n-hexane to the culture medium supernatants, in contrast to incubation with water-soluble solvents.
publisher Sociedade Brasileira de Microbiologia
publishDate 1999
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400003
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