Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum rifai

Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum rifai

The isolation of genetic markers, like drug resistance and auxotrophy, is a laborious but important step in genetic research. The isolation of auxotrophic mutants of Trichoderma harzianum using the filtration enrichment technique was more effective than using the total isolation technique. Most of 12 auxotrophic mutants exhibited similar growth rate and higher sporulation when compared with the wild type, but only two mutants (TWS-410 and TW5-523) could grow in 500µg/L of benomyl.

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Main Authors: Cassiolato,Ana Maria R., Melo,Itamar Soares de
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Microbiologia 1999
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000100008
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spelling oai:scielo:S0001-371419990001000081999-09-17Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum rifaiCassiolato,Ana Maria R.Melo,Itamar Soares de Filtration enrichment technique auxotrophic mutants Trichoderma harzianum benomyl The isolation of genetic markers, like drug resistance and auxotrophy, is a laborious but important step in genetic research. The isolation of auxotrophic mutants of Trichoderma harzianum using the filtration enrichment technique was more effective than using the total isolation technique. Most of 12 auxotrophic mutants exhibited similar growth rate and higher sporulation when compared with the wild type, but only two mutants (TWS-410 and TW5-523) could grow in 500µg/L of benomyl.info:eu-repo/semantics/openAccessSociedade Brasileira de MicrobiologiaRevista de Microbiologia v.30 n.1 19991999-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000100008en10.1590/S0001-37141999000100008
institution SCIELO
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country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Cassiolato,Ana Maria R.
Melo,Itamar Soares de
spellingShingle Cassiolato,Ana Maria R.
Melo,Itamar Soares de
Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum rifai
author_facet Cassiolato,Ana Maria R.
Melo,Itamar Soares de
author_sort Cassiolato,Ana Maria R.
title Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum rifai
title_short Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum rifai
title_full Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum rifai
title_fullStr Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum rifai
title_full_unstemmed Filtration enrichment method for isolation of auxotrophic mutants of Trichoderma harzianum rifai
title_sort filtration enrichment method for isolation of auxotrophic mutants of trichoderma harzianum rifai
description The isolation of genetic markers, like drug resistance and auxotrophy, is a laborious but important step in genetic research. The isolation of auxotrophic mutants of Trichoderma harzianum using the filtration enrichment technique was more effective than using the total isolation technique. Most of 12 auxotrophic mutants exhibited similar growth rate and higher sporulation when compared with the wild type, but only two mutants (TWS-410 and TW5-523) could grow in 500µg/L of benomyl.
publisher Sociedade Brasileira de Microbiologia
publishDate 1999
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000100008
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AT meloitamarsoaresde filtrationenrichmentmethodforisolationofauxotrophicmutantsoftrichodermaharzianumrifai
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