Genotoxicity and histopathological assessment of silver nanoparticles in Swiss albino mice
Silver nanoparticles (AgNPs) are widely used in industrial and medical applications. However, there is a growing concern about the potentialities of AgNPs to induce genotoxicity and DNA damage in humans. In this study, genotoxic and histopathological effects of AgNPs were investigated in mice using two well-characterized genetic assays: mouse bone marrow micronuclei (MN) and mouse sperm morphology assays. Swiss albino mice (total N=18) were exposed to varying concentrations (3,000mg/Kg, 4,000mg/Kg, 5,000mg/Kg and 6,000mg/Kg) of AgNPs for 5 consecutive days and observed for 30 days afterwards. Distilled water and colchicine were used as negative and positive controls, respectively. The MN assay showed that the frequency of micronuclei induction increased with AgNP concentration. Statistically significant differences (p<0,05) were observed for the micronucleus frequency in the blood erythrocytes in all the test concentrations. Sperm head morphology assay also revealed various types of abnormal sperm head morphology and there was statistically significant increase in frequency of sperm abnormalities. Histopathological profiles of the liver also showed enlarge sinusoids, irregular portal tract, and dose-dependent vacuolation. These results suggest that AgNPs is genotoxic and represent a serious health risk to human heatlh.
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Universidad Estatal a Distancia, Costa Rica
2018
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oai:revistas.investiga.uned.ac.cr:article20082022-09-12T01:48:33Z Genotoxicity and histopathological assessment of silver nanoparticles in Swiss albino mice Genotoxicidad y evaluación histopatológica de nanopartículas de plata en ratones albinos suizos. Iyiola, Oluyinka A Olafimihan, Temitope F. Sulaiman, Faoziyat A. Anifowoshe, Abass T. silver nanoparticles histopathological sperm morphology genotoxicity micronucleus nanopartículas de plata histopatológico morfología espermática genotoxicidad micronúcleo Silver nanoparticles (AgNPs) are widely used in industrial and medical applications. However, there is a growing concern about the potentialities of AgNPs to induce genotoxicity and DNA damage in humans. In this study, genotoxic and histopathological effects of AgNPs were investigated in mice using two well-characterized genetic assays: mouse bone marrow micronuclei (MN) and mouse sperm morphology assays. Swiss albino mice (total N=18) were exposed to varying concentrations (3,000mg/Kg, 4,000mg/Kg, 5,000mg/Kg and 6,000mg/Kg) of AgNPs for 5 consecutive days and observed for 30 days afterwards. Distilled water and colchicine were used as negative and positive controls, respectively. The MN assay showed that the frequency of micronuclei induction increased with AgNP concentration. Statistically significant differences (p<0,05) were observed for the micronucleus frequency in the blood erythrocytes in all the test concentrations. Sperm head morphology assay also revealed various types of abnormal sperm head morphology and there was statistically significant increase in frequency of sperm abnormalities. Histopathological profiles of the liver also showed enlarge sinusoids, irregular portal tract, and dose-dependent vacuolation. These results suggest that AgNPs is genotoxic and represent a serious health risk to human heatlh. Las nanopartículas de plata (AgNPs) son ampliamente utilizadas en la industria y la medicina. Sin embargo, existe una creciente preocupación acerca de las potencialidades de los AgNPs para inducir genotoxicidad y daño del ADN en seres humanos. En este estudio, se investigaron los efectos genotóxicos e histopatológicos de los AgNPs en ratones utilizando dos ensayos genéticos: micronúcleos de médula ósea de ratón (MN) y ensayos de morfología de espermatozoides de ratón. Un total de 16 ratones de peso medio de 25-30g se expusieron a concentraciones variables (3,000mg/Kg, 4,000mg/Kg, 5,000mg/Kg y 6,000mg/Kg) de AgNP durante 5 días consecutivos y se observaron durante 30 días. Usé agua destilada y colchicina como controles negativos y positivos, respectivamente. El ensayo MN mostró que la frecuencia de inducción de micronúcleos aumentó con lasconcentraciones de AgNPs. En todas las concentraciones de ensayo hubo diferencias estadísticamente significativas (p<0,05) en la frecuencia micronuclear de eritrocitos sanguíneos. Hubo varios tipos de morfología anormal de la cabeza del espermatozoo y aumento estadísticamente significativo en la frecuencia de anormalidades espermáticas. Los perfiles histopatológicos del hígado también mostraron sinusoides de aumento, tracto portal irregular y aparición de vacuolación dependiente de la dosis. Estos resultados sugieren que los AgNPs son genotóxicos y plantean un serio riesgo para la salud de los seres humanos considerando su uso en dispositivos médicos, hogar y varios tipos de productos de consumo. Universidad Estatal a Distancia, Costa Rica 2018-02-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html application/epub+zip https://revistas.uned.ac.cr/index.php/cuadernos/article/view/2008 10.22458/urj.v10i1.2008 UNED Research Journal; Vol. 10 No. 1 (2018) UNED Research Journal; Vol. 10 Núm. 1 (2018) 1659-441X 1659-4266 eng https://revistas.uned.ac.cr/index.php/cuadernos/article/view/2008/2251 https://revistas.uned.ac.cr/index.php/cuadernos/article/view/2008/2252 https://revistas.uned.ac.cr/index.php/cuadernos/article/view/2008/2253 Copyright (c) 2018 UNED Research Journal https://creativecommons.org/licenses/by/4.0 |
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Iyiola, Oluyinka A Olafimihan, Temitope F. Sulaiman, Faoziyat A. Anifowoshe, Abass T. |
| spellingShingle |
Iyiola, Oluyinka A Olafimihan, Temitope F. Sulaiman, Faoziyat A. Anifowoshe, Abass T. Genotoxicity and histopathological assessment of silver nanoparticles in Swiss albino mice |
| author_facet |
Iyiola, Oluyinka A Olafimihan, Temitope F. Sulaiman, Faoziyat A. Anifowoshe, Abass T. |
| author_sort |
Iyiola, Oluyinka A |
| title |
Genotoxicity and histopathological assessment of silver nanoparticles in Swiss albino mice |
| title_short |
Genotoxicity and histopathological assessment of silver nanoparticles in Swiss albino mice |
| title_full |
Genotoxicity and histopathological assessment of silver nanoparticles in Swiss albino mice |
| title_fullStr |
Genotoxicity and histopathological assessment of silver nanoparticles in Swiss albino mice |
| title_full_unstemmed |
Genotoxicity and histopathological assessment of silver nanoparticles in Swiss albino mice |
| title_sort |
genotoxicity and histopathological assessment of silver nanoparticles in swiss albino mice |
| description |
Silver nanoparticles (AgNPs) are widely used in industrial and medical applications. However, there is a growing concern about the potentialities of AgNPs to induce genotoxicity and DNA damage in humans. In this study, genotoxic and histopathological effects of AgNPs were investigated in mice using two well-characterized genetic assays: mouse bone marrow micronuclei (MN) and mouse sperm morphology assays. Swiss albino mice (total N=18) were exposed to varying concentrations (3,000mg/Kg, 4,000mg/Kg, 5,000mg/Kg and 6,000mg/Kg) of AgNPs for 5 consecutive days and observed for 30 days afterwards. Distilled water and colchicine were used as negative and positive controls, respectively. The MN assay showed that the frequency of micronuclei induction increased with AgNP concentration. Statistically significant differences (p<0,05) were observed for the micronucleus frequency in the blood erythrocytes in all the test concentrations. Sperm head morphology assay also revealed various types of abnormal sperm head morphology and there was statistically significant increase in frequency of sperm abnormalities. Histopathological profiles of the liver also showed enlarge sinusoids, irregular portal tract, and dose-dependent vacuolation. These results suggest that AgNPs is genotoxic and represent a serious health risk to human heatlh. |
| publisher |
Universidad Estatal a Distancia, Costa Rica |
| publishDate |
2018 |
| url |
https://revistas.uned.ac.cr/index.php/cuadernos/article/view/2008 |
| work_keys_str_mv |
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