Morphometric subpopulations study of white-tailed deer (Odocoileus virginianus peruvianus) epididymal spermatozoa
Introduction. The study of the sperm morphometry of the white-tailed deer (Odocoileus virginianus peruvianus) allows to characterize the sperm cells of this species for conservation purposes. Objective. To determine the sperm subpopulations according to the morphometry of the epididymal spermatozoa of the white-tailed deer, using a CASA-Morph system. Materials and methods. The study was carried out in the second semester 2016, at the Universidad Nacional San Antonio Abad del Cusco, Peru. In semen samples obtained from the caudal end of the epididymis of two deer (two and four teeth), it was determined based on the CASA-Morph system, Integrated Semen Analysis System (ISAS®v1): the length, width, area, perimeter, ellipticity, elongation, regularity, and roughness of the sperm head were determined, also, the width, area, distance, and midpiece insertion angle of the sperm. Principal components analysis (PCA) was performed and the non-hierarchical k-means model was applied to determine the optimal number of clusters. Results. The morphometric variables were distributed in five PCAs: ellipticity, size, circularity, midpiece insertion angle, which explained 81.24 % of the total variance. The Cluster analysis determined four subpopulations (SP), SP1 grouped large, elongated cells with high elongation and ellipticity (21.76 %), SP2 composed of small and thin spermatozoa both in the head and in the midpiece (37.7 %). The SP3 was related to the presence of elongated cells with high values of midpiece width, ellipticity, and elongation (17.7 %), and SP4 grouped spermatozoa with intermediate size of the head and the midpiece (22.84 %). Conclusion. The determination of four sperm subpopulations in white-tailed deer semen can be the basis of assisted reproduction programs for this species.
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Digital revista |
| Language: | spa eng |
| Published: |
Universidad de Costa Rica
2022
|
| Online Access: | https://revistas.ucr.ac.cr/index.php/agromeso/article/view/46938 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
oai:portal.ucr.ac.cr:article46938 |
|---|---|
| record_format |
ojs |
| institution |
UCR |
| collection |
OJS |
| country |
Costa Rica |
| countrycode |
CR |
| component |
Revista |
| access |
En linea |
| databasecode |
rev-agromeso |
| tag |
revista |
| region |
America Central |
| libraryname |
Bibioteca de la Facultad de Agronomía |
| language |
spa eng |
| format |
Digital |
| author |
Cucho, Hernán Nina, Gregorio Meza, Aydee Ccalta, Ruth Ordóñez, César Valverde Abarca, Anthony |
| spellingShingle |
Cucho, Hernán Nina, Gregorio Meza, Aydee Ccalta, Ruth Ordóñez, César Valverde Abarca, Anthony Morphometric subpopulations study of white-tailed deer (Odocoileus virginianus peruvianus) epididymal spermatozoa |
| author_facet |
Cucho, Hernán Nina, Gregorio Meza, Aydee Ccalta, Ruth Ordóñez, César Valverde Abarca, Anthony |
| author_sort |
Cucho, Hernán |
| title |
Morphometric subpopulations study of white-tailed deer (Odocoileus virginianus peruvianus) epididymal spermatozoa |
| title_short |
Morphometric subpopulations study of white-tailed deer (Odocoileus virginianus peruvianus) epididymal spermatozoa |
| title_full |
Morphometric subpopulations study of white-tailed deer (Odocoileus virginianus peruvianus) epididymal spermatozoa |
| title_fullStr |
Morphometric subpopulations study of white-tailed deer (Odocoileus virginianus peruvianus) epididymal spermatozoa |
| title_full_unstemmed |
Morphometric subpopulations study of white-tailed deer (Odocoileus virginianus peruvianus) epididymal spermatozoa |
| title_sort |
morphometric subpopulations study of white-tailed deer (odocoileus virginianus peruvianus) epididymal spermatozoa |
| description |
Introduction. The study of the sperm morphometry of the white-tailed deer (Odocoileus virginianus peruvianus) allows to characterize the sperm cells of this species for conservation purposes. Objective. To determine the sperm subpopulations according to the morphometry of the epididymal spermatozoa of the white-tailed deer, using a CASA-Morph system. Materials and methods. The study was carried out in the second semester 2016, at the Universidad Nacional San Antonio Abad del Cusco, Peru. In semen samples obtained from the caudal end of the epididymis of two deer (two and four teeth), it was determined based on the CASA-Morph system, Integrated Semen Analysis System (ISAS®v1): the length, width, area, perimeter, ellipticity, elongation, regularity, and roughness of the sperm head were determined, also, the width, area, distance, and midpiece insertion angle of the sperm. Principal components analysis (PCA) was performed and the non-hierarchical k-means model was applied to determine the optimal number of clusters. Results. The morphometric variables were distributed in five PCAs: ellipticity, size, circularity, midpiece insertion angle, which explained 81.24 % of the total variance. The Cluster analysis determined four subpopulations (SP), SP1 grouped large, elongated cells with high elongation and ellipticity (21.76 %), SP2 composed of small and thin spermatozoa both in the head and in the midpiece (37.7 %). The SP3 was related to the presence of elongated cells with high values of midpiece width, ellipticity, and elongation (17.7 %), and SP4 grouped spermatozoa with intermediate size of the head and the midpiece (22.84 %). Conclusion. The determination of four sperm subpopulations in white-tailed deer semen can be the basis of assisted reproduction programs for this species. |
| publisher |
Universidad de Costa Rica |
| publishDate |
2022 |
| url |
https://revistas.ucr.ac.cr/index.php/agromeso/article/view/46938 |
| work_keys_str_mv |
AT cuchohernan morphometricsubpopulationsstudyofwhitetaileddeerodocoileusvirginianusperuvianusepididymalspermatozoa AT ninagregorio morphometricsubpopulationsstudyofwhitetaileddeerodocoileusvirginianusperuvianusepididymalspermatozoa AT mezaaydee morphometricsubpopulationsstudyofwhitetaileddeerodocoileusvirginianusperuvianusepididymalspermatozoa AT ccaltaruth morphometricsubpopulationsstudyofwhitetaileddeerodocoileusvirginianusperuvianusepididymalspermatozoa AT ordonezcesar morphometricsubpopulationsstudyofwhitetaileddeerodocoileusvirginianusperuvianusepididymalspermatozoa AT valverdeabarcaanthony morphometricsubpopulationsstudyofwhitetaileddeerodocoileusvirginianusperuvianusepididymalspermatozoa AT cuchohernan subpoblacionesmorfometricasdeespermatozoidesepididimariosdelvenadodecolablancaodocoileusvirginianusperuvianus AT ninagregorio subpoblacionesmorfometricasdeespermatozoidesepididimariosdelvenadodecolablancaodocoileusvirginianusperuvianus AT mezaaydee subpoblacionesmorfometricasdeespermatozoidesepididimariosdelvenadodecolablancaodocoileusvirginianusperuvianus AT ccaltaruth subpoblacionesmorfometricasdeespermatozoidesepididimariosdelvenadodecolablancaodocoileusvirginianusperuvianus AT ordonezcesar subpoblacionesmorfometricasdeespermatozoidesepididimariosdelvenadodecolablancaodocoileusvirginianusperuvianus AT valverdeabarcaanthony subpoblacionesmorfometricasdeespermatozoidesepididimariosdelvenadodecolablancaodocoileusvirginianusperuvianus |
| _version_ |
1769606468056645632 |
| spelling |
oai:portal.ucr.ac.cr:article469382023-06-16T13:43:39Z Morphometric subpopulations study of white-tailed deer (Odocoileus virginianus peruvianus) epididymal spermatozoa Subpoblaciones morfométricas de espermatozoides epididimarios del venado de cola blanca (Odocoileus virginianus peruvianus) Cucho, Hernán Nina, Gregorio Meza, Aydee Ccalta, Ruth Ordóñez, César Valverde Abarca, Anthony deer semen CASA morph animal reproduction wildanimal genetic resources semen de venado CASA morph reproducción aniaml recursos genéticos silvestres de animales Introduction. The study of the sperm morphometry of the white-tailed deer (Odocoileus virginianus peruvianus) allows to characterize the sperm cells of this species for conservation purposes. Objective. To determine the sperm subpopulations according to the morphometry of the epididymal spermatozoa of the white-tailed deer, using a CASA-Morph system. Materials and methods. The study was carried out in the second semester 2016, at the Universidad Nacional San Antonio Abad del Cusco, Peru. In semen samples obtained from the caudal end of the epididymis of two deer (two and four teeth), it was determined based on the CASA-Morph system, Integrated Semen Analysis System (ISAS®v1): the length, width, area, perimeter, ellipticity, elongation, regularity, and roughness of the sperm head were determined, also, the width, area, distance, and midpiece insertion angle of the sperm. Principal components analysis (PCA) was performed and the non-hierarchical k-means model was applied to determine the optimal number of clusters. Results. The morphometric variables were distributed in five PCAs: ellipticity, size, circularity, midpiece insertion angle, which explained 81.24 % of the total variance. The Cluster analysis determined four subpopulations (SP), SP1 grouped large, elongated cells with high elongation and ellipticity (21.76 %), SP2 composed of small and thin spermatozoa both in the head and in the midpiece (37.7 %). The SP3 was related to the presence of elongated cells with high values of midpiece width, ellipticity, and elongation (17.7 %), and SP4 grouped spermatozoa with intermediate size of the head and the midpiece (22.84 %). Conclusion. The determination of four sperm subpopulations in white-tailed deer semen can be the basis of assisted reproduction programs for this species. Introducción. El estudio de la morfometría espermática del venado cola blanca (Odocoileus virginianus peruvianus) permite caracterizar las células espermáticas de esta especie con propósitos de conservación. Objetivo. Determinar las subpoblaciones espermáticas según la morfometría de los espermatozoides epididimarios del venado de cola blanca, con un sistema CASA-Morph. Materiales y métodos. El estudio se desarrolló en el segundo semestre 2016, en la Universidad Nacional de San Antonio Abad del Cusco, Perú. En muestras de semen obtenidas del extremo caudal del epidídimo de dos venados (dos y cuatro dientes), se determinó con base en el sistema CASA-Morph, Integrated Semen Analysis System (ISAS®v1): la longitud, anchura, área, perímetro, elipticidad, elongación, regularidad y rugosidad de la cabeza del espermatozoide, también, la anchura, área, distancia y ángulo de inserción de la pieza intermedia. Se realizó un análisis de componentes principales (PCA) y se aplicó el modelo no jerárquico k-medias para determinar el número óptimo de clústeres. Resultados. Las variables morfométricas se distribuyeron en cinco PCA: elipticidad, tamaño, circularidad, ángulo de inserción de la pieza intermedia y anchura de la pieza intermedia, que explicaron un 81,24 % de la varianza total. El análisis de clústeres determinó cuatro subpoblaciones (SP), la SP1 agrupó células grandes, alargadas con alta elongación y elipticidad (21,76 %), la SP2 compuesta por espermatozoides de tamaño pequeño y delgados tanto en la cabeza como en la pieza intermedia (37,7 %), la SP3 se relacionó con la presencia de células alargadas con valores altos de anchura de la pieza intermedia, elipticidad y elongación (17,7 %), y la SP4 agrupó espermatozoides de tamaño intermedio de la cabeza y de la pieza intermedia (22,84 %). Conclusiones. La determinación de cuatro subpoblaciones espermáticas en el semen de venado cola blanca puede ser la base de programas de reproducción asistida de esta especie. Universidad de Costa Rica 2022-01-21 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Article text/xml application/pdf application/epub+zip text/html audio/mpeg audio/mpeg https://revistas.ucr.ac.cr/index.php/agromeso/article/view/46938 10.15517/am.v33i2.46938 Agronomía Mesoamericana; 2022: Agronomía Mesoamericana: Vol. 33, Issue 2 (May-August).; 46938 Agronomía Mesoamericana; 2022: Agronomía Mesoamericana: Vol. 33, Nº 2 (mayo-agosto).; 46938 Agronomía Mesoamericana; 2022: Agronomía Mesoamericana: Vol. 33, Issue 2 (May-August).; 46938 2215-3608 1021-7444 spa eng https://revistas.ucr.ac.cr/index.php/agromeso/article/view/46938/49835 https://revistas.ucr.ac.cr/index.php/agromeso/article/view/46938/49836 https://revistas.ucr.ac.cr/index.php/agromeso/article/view/46938/49837 https://revistas.ucr.ac.cr/index.php/agromeso/article/view/46938/49838 https://revistas.ucr.ac.cr/index.php/agromeso/article/view/46938/49839 https://revistas.ucr.ac.cr/index.php/agromeso/article/view/46938/49840 Copyright (c) 2022 Hernán Cucho, Gregorio Nina, Aydee Meza, Ruth Ccalta, César Ordóñez, Anthony Valverde Abarca |