In vitro Organogenesis of Stevia rebaudiana Bert. with Different Explants and Growth Regulators
Objective: To evaluate various explants and growth regulators to improve the in vitro propagation of Stevia rebaudiana via organogenesis. Design / methodology / approach: Explants and growth regulators were evaluated at two concentrations. The explants were, nodal segment, axillary bud and apical meristem and the growth regulators Benzylaminopurine (BAP) 1,125 mgL-1 and 3.0 mgL-1, Naphthaleneacetic Acid (ANA) 1.5 mgL-1 and 3.0 mgL-1, and a Brassinosteroid CIDEF -4 (Br) 1.0 mgL-1 and 1.5 mgL-1. In total 18 treatments with 7 repetitions. In the induction, contamination, oxidation and survival were registered and in multiplication, number of leaves, height of regrowth and presence of roots. Results: Differential response between explants was presented according to ontogenetic age in the induction and multiplication stage, the morphological components presented differences between the concentrations of growth regulators and explants with greater effectiveness when adding BAP in apical meristem. Study limitations / implications: The origin and age of the explants can induce differential growth in interaction with growth regulators. Findings / conclusions: The apical meristem explant has better advantages for the in vitro reproduction of Stevia rebaudiana by expressing less contamination and greater survival in the induction stage, even when it presented the highest oxidation of the explants, it did not influence the decrease in the survival of the same explants. Likewise, in the multiplication stage with apical meristem, the height, the number of leaves and the presence of roots increased. The values ??were more outstanding in interaction with BAP
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Colegio de Postgraduados
2021
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Aguirre-Medina, Juan Francisco Gálvez-López, Ana Laura Aguirre-Cadena, Juan Francisco |
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Aguirre-Medina, Juan Francisco Gálvez-López, Ana Laura Aguirre-Cadena, Juan Francisco In vitro Organogenesis of Stevia rebaudiana Bert. with Different Explants and Growth Regulators |
| author_facet |
Aguirre-Medina, Juan Francisco Gálvez-López, Ana Laura Aguirre-Cadena, Juan Francisco |
| author_sort |
Aguirre-Medina, Juan Francisco |
| title |
In vitro Organogenesis of Stevia rebaudiana Bert. with Different Explants and Growth Regulators |
| title_short |
In vitro Organogenesis of Stevia rebaudiana Bert. with Different Explants and Growth Regulators |
| title_full |
In vitro Organogenesis of Stevia rebaudiana Bert. with Different Explants and Growth Regulators |
| title_fullStr |
In vitro Organogenesis of Stevia rebaudiana Bert. with Different Explants and Growth Regulators |
| title_full_unstemmed |
In vitro Organogenesis of Stevia rebaudiana Bert. with Different Explants and Growth Regulators |
| title_sort |
in vitro organogenesis of stevia rebaudiana bert. with different explants and growth regulators |
| description |
Objective: To evaluate various explants and growth regulators to improve the in vitro propagation of Stevia rebaudiana via organogenesis.
Design / methodology / approach: Explants and growth regulators were evaluated at two concentrations. The explants were, nodal segment, axillary bud and apical meristem and the growth regulators Benzylaminopurine (BAP) 1,125 mgL-1 and 3.0 mgL-1, Naphthaleneacetic Acid (ANA) 1.5 mgL-1 and 3.0 mgL-1, and a Brassinosteroid CIDEF -4 (Br) 1.0 mgL-1 and 1.5 mgL-1. In total 18 treatments with 7 repetitions. In the induction, contamination, oxidation and survival were registered and in multiplication, number of leaves, height of regrowth and presence of roots.
Results: Differential response between explants was presented according to ontogenetic age in the induction and multiplication stage, the morphological components presented differences between the concentrations of growth regulators and explants with greater effectiveness when adding BAP in apical meristem.
Study limitations / implications: The origin and age of the explants can induce differential growth in interaction with growth regulators.
Findings / conclusions: The apical meristem explant has better advantages for the in vitro reproduction of Stevia rebaudiana by expressing less contamination and greater survival in the induction stage, even when it presented the highest oxidation of the explants, it did not influence the decrease in the survival of the same explants. Likewise, in the multiplication stage with apical meristem, the height, the number of leaves and the presence of roots increased. The values ??were more outstanding in interaction with BAP |
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Colegio de Postgraduados |
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2021 |
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https://revista-agroproductividad.org/index.php/agroproductividad/article/view/1934 |
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AT aguirremedinajuanfrancisco invitroorganogenesisofsteviarebaudianabertwithdifferentexplantsandgrowthregulators AT galvezlopezanalaura invitroorganogenesisofsteviarebaudianabertwithdifferentexplantsandgrowthregulators AT aguirrecadenajuanfrancisco invitroorganogenesisofsteviarebaudianabertwithdifferentexplantsandgrowthregulators |
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oai:ojs.pkp.sfu.ca:article19342021-04-19T22:15:43Z In vitro Organogenesis of Stevia rebaudiana Bert. with Different Explants and Growth Regulators Aguirre-Medina, Juan Francisco Gálvez-López, Ana Laura Aguirre-Cadena, Juan Francisco Estevia, Micropropagation, Brassinosteroid stevia, micropropagation, brassinosteroids. Objective: To evaluate various explants and growth regulators to improve the in vitro propagation of Stevia rebaudiana via organogenesis. Design / methodology / approach: Explants and growth regulators were evaluated at two concentrations. The explants were, nodal segment, axillary bud and apical meristem and the growth regulators Benzylaminopurine (BAP) 1,125 mgL-1 and 3.0 mgL-1, Naphthaleneacetic Acid (ANA) 1.5 mgL-1 and 3.0 mgL-1, and a Brassinosteroid CIDEF -4 (Br) 1.0 mgL-1 and 1.5 mgL-1. In total 18 treatments with 7 repetitions. In the induction, contamination, oxidation and survival were registered and in multiplication, number of leaves, height of regrowth and presence of roots. Results: Differential response between explants was presented according to ontogenetic age in the induction and multiplication stage, the morphological components presented differences between the concentrations of growth regulators and explants with greater effectiveness when adding BAP in apical meristem. Study limitations / implications: The origin and age of the explants can induce differential growth in interaction with growth regulators. Findings / conclusions: The apical meristem explant has better advantages for the in vitro reproduction of Stevia rebaudiana by expressing less contamination and greater survival in the induction stage, even when it presented the highest oxidation of the explants, it did not influence the decrease in the survival of the same explants. Likewise, in the multiplication stage with apical meristem, the height, the number of leaves and the presence of roots increased. The values ??were more outstanding in interaction with BAP Objective: To evaluate various explants and growth regulators in order to improve invitro propagation of Stevia rebaudiana through organogenesis.Design/Methodology/Approach: Explants and growth regulators in two differentconcentrations were evaluated. The explants were nodal segment, axillary bud, andapical meristem; while the growth regulators were benzylaminopurine (BAP) at 1.125 mgL -1 and 3.0 mg L -1 , naphthaleneacetic acid (NAA) at 1.5 mg L -1 and 3.0 mg L -1 , andCIDEF-4 brassinosteroids (BRs) at 1.0 mg L -1 and 1.5 mg L -1 . In total 18 treatments withseven repetitions. Contamination, oxidation, and survival were recorded duringinduction; while leaf number, regrowth height, and root presence were recorded duringmultiplication.Results: At the induction stage there was a differential response between explantsaccording to their ontogenetic age; during multiplication, the morphological componentsshowed differences between concentrations of growth regulators and explants, withhigher effectiveness when adding BAP to apical meristems.Study Limitations/Implications: Both the origin and the age of explants can inducedifferential growth while interacting with growth regulators. Findings/Conclusions: Apical meristem explants showed better advantages for in vitroreproduction of S. rebaudiana since they present less contamination and higher survivalat the induction stage, even when exhibiting the highest oxidation among explants,which did not influence the decrease in their survival. At the multiplication stage withapical meristem, height, leaf number, and root presence were increased. Values werehigh when interacting with BAP. Colegio de Postgraduados 2021-04-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revista-agroproductividad.org/index.php/agroproductividad/article/view/1934 10.32854/agrop.v14i3.1934 AgroP; Vol. 14 No. 3 (2021): MARZO Agro Productividad; Vol. 14 Núm. 3 (2021): MARZO 2594-0252 2448-7546 spa https://revista-agroproductividad.org/index.php/agroproductividad/article/view/1934/1502 Derechos de autor 2021 Agro Productividad |