Survival of the sclerotia of Phymatotrichopsis omnivora as a function of pH in vitro

Survival of the sclerotia of Phymatotrichopsis omnivora as a function of pH in vitro

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Phymatotrichopsis omnivora is a phytopathogenic fungus that attacks thousands of species of plants, mainly in alkaline soils. In this study, we assessed the capacity of sclerotia of P. omnivora to change the pH and survive in acidic and buffer solutions. The sclerotia were placed in acetic (0.4, 0.8 and 1.7 mmol L-1) and sulfuric acid solutions (0.25, 0.5 and 1 mmol L-1) for two weeks; these solutions were not replaced or were remade one, two or three times. The sclerotia were also put in buffer solutions for seven days (pH 4, 5, 8 and 9), undiluted or diluted at 1:2, 1:4 and 1:10 v/v. Other sclerotia were placed in selected solutions of 0.5 mmol L-1 sulfuric acid and in buffer solutions with pH 5 diluted 1:10; they were then extracted and subjected to stress treatments. These treatments consisted in placing the sclerotia in: NaOCl solution, sand saturated with glucose, f looded sand, and sand at field capacity. The sclerotia changed the pH of the acetic acid solutions without replacement, and the Ph of buffer solutions with pH 8-9 diluted 1:10. However, the change of pH of the solutions was marginal when the acids were not replaced and when the buffer solutions were not diluted. The survival of the sclerotia was null with three replacements of sulfuric acid at 1 mmol L-1 and in buffer solutions with pH 4 and 5 diluted 1:4 or less, and with pH 9 undiluted; some sclerotia survived in the rest of the solutions. Stress treatments had a synergistic effect of reducing the survival of sclerotia that had been previously placed in the selected solutions (0.5 mmol L-1 sulfuric acid and buffer with pH 5 diluted 1:10).

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Main Author: Samaniego Gaxiola, José Alfredo
Format: Digital revista
Language:spa
Published: Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias 2018
Online Access:https://cienciasagricolas.inifap.gob.mx/index.php/agricolas/article/view/1197
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id oai:ojs.pkp.sfu.ca:article1197
record_format ojs
institution INIFAP
collection OJS
country México
countrycode MX
component Revista
access En linea
databasecode rev-remexca
tag revista
region America del Norte
libraryname Biblioteca INIFAP
language spa
format Digital
author Samaniego Gaxiola, José Alfredo
spellingShingle Samaniego Gaxiola, José Alfredo
Survival of the sclerotia of Phymatotrichopsis omnivora as a function of pH in vitro
author_facet Samaniego Gaxiola, José Alfredo
author_sort Samaniego Gaxiola, José Alfredo
title Survival of the sclerotia of Phymatotrichopsis omnivora as a function of pH in vitro
title_short Survival of the sclerotia of Phymatotrichopsis omnivora as a function of pH in vitro
title_full Survival of the sclerotia of Phymatotrichopsis omnivora as a function of pH in vitro
title_fullStr Survival of the sclerotia of Phymatotrichopsis omnivora as a function of pH in vitro
title_full_unstemmed Survival of the sclerotia of Phymatotrichopsis omnivora as a function of pH in vitro
title_sort survival of the sclerotia of phymatotrichopsis omnivora as a function of ph in vitro
description Phymatotrichopsis omnivora is a phytopathogenic fungus that attacks thousands of species of plants, mainly in alkaline soils. In this study, we assessed the capacity of sclerotia of P. omnivora to change the pH and survive in acidic and buffer solutions. The sclerotia were placed in acetic (0.4, 0.8 and 1.7 mmol L-1) and sulfuric acid solutions (0.25, 0.5 and 1 mmol L-1) for two weeks; these solutions were not replaced or were remade one, two or three times. The sclerotia were also put in buffer solutions for seven days (pH 4, 5, 8 and 9), undiluted or diluted at 1:2, 1:4 and 1:10 v/v. Other sclerotia were placed in selected solutions of 0.5 mmol L-1 sulfuric acid and in buffer solutions with pH 5 diluted 1:10; they were then extracted and subjected to stress treatments. These treatments consisted in placing the sclerotia in: NaOCl solution, sand saturated with glucose, f looded sand, and sand at field capacity. The sclerotia changed the pH of the acetic acid solutions without replacement, and the Ph of buffer solutions with pH 8-9 diluted 1:10. However, the change of pH of the solutions was marginal when the acids were not replaced and when the buffer solutions were not diluted. The survival of the sclerotia was null with three replacements of sulfuric acid at 1 mmol L-1 and in buffer solutions with pH 4 and 5 diluted 1:4 or less, and with pH 9 undiluted; some sclerotia survived in the rest of the solutions. Stress treatments had a synergistic effect of reducing the survival of sclerotia that had been previously placed in the selected solutions (0.5 mmol L-1 sulfuric acid and buffer with pH 5 diluted 1:10).
publisher Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias
publishDate 2018
url https://cienciasagricolas.inifap.gob.mx/index.php/agricolas/article/view/1197
work_keys_str_mv AT samaniegogaxiolajosealfredo survivalofthesclerotiaofphymatotrichopsisomnivoraasafunctionofphinvitro
AT samaniegogaxiolajosealfredo supervivenciadelosesclerociosdephymatotrichopsisomnivoraenfunciondelphinvitro
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spelling oai:ojs.pkp.sfu.ca:article11972018-05-28T16:15:15Z Survival of the sclerotia of Phymatotrichopsis omnivora as a function of pH in vitro Supervivencia de los esclerocios de Phymatotrichopsis omnivora en función del pH in vitro Samaniego Gaxiola, José Alfredo ácidos grasos volátiles hongos fitopatógenos en suelo materia orgánica organic matter phytopathogenic fungi in soil volatile fatty acids Phymatotrichopsis omnivora is a phytopathogenic fungus that attacks thousands of species of plants, mainly in alkaline soils. In this study, we assessed the capacity of sclerotia of P. omnivora to change the pH and survive in acidic and buffer solutions. The sclerotia were placed in acetic (0.4, 0.8 and 1.7 mmol L-1) and sulfuric acid solutions (0.25, 0.5 and 1 mmol L-1) for two weeks; these solutions were not replaced or were remade one, two or three times. The sclerotia were also put in buffer solutions for seven days (pH 4, 5, 8 and 9), undiluted or diluted at 1:2, 1:4 and 1:10 v/v. Other sclerotia were placed in selected solutions of 0.5 mmol L-1 sulfuric acid and in buffer solutions with pH 5 diluted 1:10; they were then extracted and subjected to stress treatments. These treatments consisted in placing the sclerotia in: NaOCl solution, sand saturated with glucose, f looded sand, and sand at field capacity. The sclerotia changed the pH of the acetic acid solutions without replacement, and the Ph of buffer solutions with pH 8-9 diluted 1:10. However, the change of pH of the solutions was marginal when the acids were not replaced and when the buffer solutions were not diluted. The survival of the sclerotia was null with three replacements of sulfuric acid at 1 mmol L-1 and in buffer solutions with pH 4 and 5 diluted 1:4 or less, and with pH 9 undiluted; some sclerotia survived in the rest of the solutions. Stress treatments had a synergistic effect of reducing the survival of sclerotia that had been previously placed in the selected solutions (0.5 mmol L-1 sulfuric acid and buffer with pH 5 diluted 1:10). Phymatotrichopsisomnivoraesunhongofitopatógenosque ataca a miles de especies de plantas, principalmente en suelos alcalinos. En el presente trabajo, se evaluó la capacidad de los esclerocios de P. omnivora para cambiar el pH y sobrevivir en soluciones ácidas y amortiguadoras. Los esclerocios fueron colocados dos semanas en soluciones de ácidos acético (0.4, 0.8 y 1.7 mmol L-1) y sulfúrico (0.25, 0.5 y 1 mmol L-1), éstas soluciones no se recambiaron o se hicieron una, dos o tres veces. Los esclerocios también se pusieron siete días en soluciones amortiguadoras (pH 4, 5, 8 y 9) sin diluir o diluidas a razón de 1:2, 1:4 y 1:10 v/v. Otros esclerocios se colocaron en soluciones selectas de ácido sulfúrico 0.5 mmol L-1 y amortiguadora a pH 5 diluida 1:10, luego se extrajeron y fueron sometieron a tratamientos de estrés. Estos tratamientos consintieron en colocar los esclerocios en: solución de NaOCl, arena inundada con glucosa, arena inundada, y arena a capacidad de campo. Los esclerocios cambiaron el pH de las soluciones de ácido acético sin recambio, y amortiguadoras de pH 8 y 9 diluidas 1:10. Pero, el cambio de pH de las soluciones fue marginal cuando los ácidos no se recambiaron y las soluciones amortiguadoras no se diluyeron. La supervivencia de los esclerocios fue nula con tres recambios de de ácido sulfúrico a l mmol L-1 y en soluciones amortiguadora de pH 4, pH 5 diluida 1:4 o menos, y pH 9 sin diluir; en el resto de las soluciones, algunos esclerocios lograron sobrevivir. Los tratamientos de estés tuvieron un efecto sinérgicos en disminuir la supervivencia de los esclerocios que previamente fueron colocados en las soluciones selectas (ácido sulfúrico 0.5 mmol L-1 y amortiguadora a pH 5 diluida 1:10). Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias 2018-05-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://cienciasagricolas.inifap.gob.mx/index.php/agricolas/article/view/1197 10.29312/remexca.v4i3.1197 Revista Mexicana de Ciencias Agrícolas; Vol. 4 No. 3 (2013); 337-351 Revista Mexicana de Ciencias Agrícolas; Vol. 4 Núm. 3 (2013); 337-351 2007-9230 2007-0934 spa https://cienciasagricolas.inifap.gob.mx/index.php/agricolas/article/view/1197/1045 Derechos de autor 2013 Revista Mexicana de Ciencias Agrícolas

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