FR 32. In vitro heterologous fertilization of buffalo oocytes
This study was designed to evaluate both a homologous and heterologous system utilizing frozen semen Bos taurus, Bos indicus y Bos bubalis. Bovine and buffaloes ovaries were obtained from a slaughterhouse and transported to the laboratory in saline solution. A total of 120 bovine and 30 buffalo oocytes were collected from follicles of different sizes. They were cultured in a 35 mm petri dish containing TCM-199 for 24 h. There was a significant (P<.05) effect of type of oocyte on fertilization rate. There were more cow oocytes fertilized between 24 and 48 h (68 %) than buffalo oocytes (47%). However, there were not significant effects (P>.05) due to type of semen. In conclusion, this study indicates that it is feasible to incorporate the bovine oocyte system as a laboratory tool to evaluate the fertility potential of semen from Bos indicus, Bos taurus and Bos bubalis breeds.
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Digital revista |
| Language: | spa |
| Published: |
Asociacion Latinoamericana de Produccion Animal
2005
|
| Online Access: | https://ojs.alpa.uy/index.php/ojs_files/article/view/297 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
oai:ojs.ojs.alpa.uy:article-297 |
|---|---|
| record_format |
ojs |
| spelling |
oai:ojs.ojs.alpa.uy:article-2972022-11-01T17:45:36Z FR 32. In vitro heterologous fertilization of buffalo oocytes Bastidas, Pedro Fernandez, Adriana Troconiz, Juan In vitro fertilization heterologous buffalo This study was designed to evaluate both a homologous and heterologous system utilizing frozen semen Bos taurus, Bos indicus y Bos bubalis. Bovine and buffaloes ovaries were obtained from a slaughterhouse and transported to the laboratory in saline solution. A total of 120 bovine and 30 buffalo oocytes were collected from follicles of different sizes. They were cultured in a 35 mm petri dish containing TCM-199 for 24 h. There was a significant (P<.05) effect of type of oocyte on fertilization rate. There were more cow oocytes fertilized between 24 and 48 h (68 %) than buffalo oocytes (47%). However, there were not significant effects (P>.05) due to type of semen. In conclusion, this study indicates that it is feasible to incorporate the bovine oocyte system as a laboratory tool to evaluate the fertility potential of semen from Bos indicus, Bos taurus and Bos bubalis breeds. Asociacion Latinoamericana de Produccion Animal 2005-10-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo cientifico original Texto application/pdf https://ojs.alpa.uy/index.php/ojs_files/article/view/297 Latin American Archives of Animal Production; Vol. 5 No. 3 (1997): Proceedings XV ALPA meeting 2 Archivos Latinoamericanos de Producción Animal; Vol. 5 Núm. 3 (1997): Proceedings XV ALPA meeting 2 2075-8359 1022-1301 spa https://ojs.alpa.uy/index.php/ojs_files/article/view/297/274 |
| institution |
ALPA |
| collection |
OJS |
| country |
Uruguay |
| countrycode |
UY |
| component |
Revista |
| access |
En linea |
| databasecode |
rev-alpa |
| tag |
revista |
| region |
America del Sur |
| libraryname |
Biblioteca ALPA |
| language |
spa |
| format |
Digital |
| author |
Bastidas, Pedro Fernandez, Adriana Troconiz, Juan |
| spellingShingle |
Bastidas, Pedro Fernandez, Adriana Troconiz, Juan FR 32. In vitro heterologous fertilization of buffalo oocytes |
| author_facet |
Bastidas, Pedro Fernandez, Adriana Troconiz, Juan |
| author_sort |
Bastidas, Pedro |
| title |
FR 32. In vitro heterologous fertilization of buffalo oocytes |
| title_short |
FR 32. In vitro heterologous fertilization of buffalo oocytes |
| title_full |
FR 32. In vitro heterologous fertilization of buffalo oocytes |
| title_fullStr |
FR 32. In vitro heterologous fertilization of buffalo oocytes |
| title_full_unstemmed |
FR 32. In vitro heterologous fertilization of buffalo oocytes |
| title_sort |
fr 32. in vitro heterologous fertilization of buffalo oocytes |
| description |
This study was designed to evaluate both a homologous and heterologous system utilizing frozen semen Bos taurus, Bos indicus y Bos bubalis. Bovine and buffaloes ovaries were obtained from a slaughterhouse and transported to the laboratory in saline solution. A total of 120 bovine and 30 buffalo oocytes were collected from follicles of different sizes. They were cultured in a 35 mm petri dish containing TCM-199 for 24 h. There was a significant (P<.05) effect of type of oocyte on fertilization rate. There were more cow oocytes fertilized between 24 and 48 h (68 %) than buffalo oocytes (47%). However, there were not significant effects (P>.05) due to type of semen. In conclusion, this study indicates that it is feasible to incorporate the bovine oocyte system as a laboratory tool to evaluate the fertility potential of semen from Bos indicus, Bos taurus and Bos bubalis breeds. |
| publisher |
Asociacion Latinoamericana de Produccion Animal |
| publishDate |
2005 |
| url |
https://ojs.alpa.uy/index.php/ojs_files/article/view/297 |
| work_keys_str_mv |
AT bastidaspedro fr32invitroheterologousfertilizationofbuffalooocytes AT fernandezadriana fr32invitroheterologousfertilizationofbuffalooocytes AT troconizjuan fr32invitroheterologousfertilizationofbuffalooocytes |
| _version_ |
1755914887886798848 |