Isolation and characterization of a myotoxin from Bothrops brazili Hoge, 1953 Hoge, 1953 snake venom (Ophidia: Viperidae).
A myotoxin from the venom of the snake Bothrops brazili has been purified by ion-exchange chromatography on CM-Sephadex C-50 with 0,05 M ammonium acetate buffer pH 7. The homogeneity was evaluated by PAGE with and without SDS, immunodiffusion and immunoelectrophoresis. The myotoxin is a basic protein with 15,6% of Lys+Arg; it is not a glicoprotein, has not enzymatic activity, and corresponds to 25% of the whole venom protein. The molecular weight of the myotoxin was determined by PAGE-SDS and gel filtration chromatography. The myotoxin has 30 KDa of molecular weight and two polypeptide chains of 15 KDa each. Myotoxin produces a severe necrosis on the gastrocnemius muscle of white mice. The myotoxin does not have hemolytic nor anticoagulant activity. However, produces edema with a DEM of 32,6 mg of protein.
Main Authors: | Pantigoso, Carmen, Escobar, Enrique, Yarlequé, Armando |
---|---|
Format: | Digital revista |
Language: | spa |
Published: |
Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
2001
|
Online Access: | https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/6716 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Action of the myotoxin from Bothrops brazili Hoge, 1953 snake venom (Ophidia: Viperidae)
by: Pantigoso, Carmen, et al.
Published: (2002) -
Isolation and partial characterization of a myotoxin from Bothrops atrox snake venom (Ophidia: Viperidae)
by: Huatuco, Sergio, et al.
Published: (2004) -
Bothrops sanctaecrucis Hoge 1966 (Squamata: Viperidae)
by: Miranda Calle, A.B., et al. -
Venomics and antivenomics of the poorly studied Brazil’s lancehead, Bothrops brazili (Hoge, 1954), from the Brazilian State of Pará
by: Sanz,Libia, et al.
Published: (2020) -
The occurrence of Crepidobothrium sp. (Cestoda, Proteocephalidae) in Bothrops moojeni (Hoge) (Serpentes, Viperidae)
by: Silva,Reinaldo José da, et al.
Published: (2001)