Male germinal epithelium recovery in mice treated with only dose of Busulfan
The DNA replication is disrupted by Busulfan which is a bifunctional alquilant as an ester agent. High dose of this compound are applied before transplants of bone marrow and germ cells. However quantification of the damage to the reproductive system and its recovery have not been established. The aim of this study was to quantify both spermatogenesis and spermiogenesis recovery in mice from damage caused by an injection of Busulfan. We used Balb C mice which were evaluated 30, 54 and 72 days after the intraperitoneal injection of 40 mg/kg body weight. We took the following variables: testicular weight, spermatic concentration in epididimus line and histological cuts to measure the diameter of the seminiferous tubules. Results were analyzed with the test U Mann-Whitney p>0.05. By day 30, testicular weight, spermatic concentration and diameter of the seminiferous tubules were reduced to 77,8%, 97,9% and 34,3% respectively compared to the controls. At day 54, testicular weight and diameter of the seminiferous tubules showed a recovery of 70%. Recovery was complete by day 72. Therefore Busulfan applied in a sigle dose, affects significantly spermatogenesis and the completed recovery takes two spermatic cycles in mice.
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Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
2005
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oai:ojs.csi.unmsm:article23682020-05-25T13:41:52Z Male germinal epithelium recovery in mice treated with only dose of Busulfan Recuperación del epitelio germinal masculino de ratones tratados con dosis única de Busulfán Guzmán, Luis López, Rosmary Llerena, Guillermo Pino, José Retuerto, Fernando Busulfán ratón espermatogénesis recuperación Busulfan mouse spermatogenesis recovery The DNA replication is disrupted by Busulfan which is a bifunctional alquilant as an ester agent. High dose of this compound are applied before transplants of bone marrow and germ cells. However quantification of the damage to the reproductive system and its recovery have not been established. The aim of this study was to quantify both spermatogenesis and spermiogenesis recovery in mice from damage caused by an injection of Busulfan. We used Balb C mice which were evaluated 30, 54 and 72 days after the intraperitoneal injection of 40 mg/kg body weight. We took the following variables: testicular weight, spermatic concentration in epididimus line and histological cuts to measure the diameter of the seminiferous tubules. Results were analyzed with the test U Mann-Whitney p>0.05. By day 30, testicular weight, spermatic concentration and diameter of the seminiferous tubules were reduced to 77,8%, 97,9% and 34,3% respectively compared to the controls. At day 54, testicular weight and diameter of the seminiferous tubules showed a recovery of 70%. Recovery was complete by day 72. Therefore Busulfan applied in a sigle dose, affects significantly spermatogenesis and the completed recovery takes two spermatic cycles in mice. Busulfán es un agente alquilante bifuncional tipo-éster, que interfiere con la replicación del ADN. Dosis elevadas de este compuesto son aplicadas antes de los transplantes de médula ósea y de células germinales, sin embargo no han sido cuantificados el daño ni la recuperación del sistema reproductor. Para cuantificar la recuperación del efecto en la espermatogénesis, se usaron ratones de la cepa Balb C, a los que se les inyectó Busulfán intraperitonealmente, 40 mg/kg de peso corporal, y se evaluó el peso testicular y la concentración espermática en cola de epidídimo, a los 30, 54 y 72 días y se realizaron cortes histológicos de testículo. Los resultados fueron analizados con la prueba U Mann-Whitney p>0.05. A los 30 días el peso testicular, la concentración espermática y el diámetro de los túbulos seminíferos presentaron una drástica reducción del 77,8%, 97,9% y 34,3% respectivamente, comparados con el grupo control. A los 54 días, el peso testicular y el diámetro testicular muestran una recuperación del 70%. A los 72 días, la recuperación es completa en los parámetros evaluados. Por lo tanto, Busulfán aplicado en una sola dosis afecta significativamente la espermatogénesis en el ratón y se logra la recuperación completa en dos ciclos espermáticos. Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas 2005-07-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article info:eu-repo/semantics/article application/pdf https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2368 10.15381/rpb.v12i1.2368 Revista Peruana de Biología; Vol. 12 No. 1 (2005); 141-144 Revista Peruana de Biología; Vol. 12 Núm. 1 (2005); 141-144 1727-9933 1561-0837 spa https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2368/2067 Derechos de autor 2005 Luis Guzmán, Rosmary López, Guillermo Llerena, José Pino, Fernando Retuerto https://creativecommons.org/licenses/by-nc-sa/4.0 |
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| author |
Guzmán, Luis López, Rosmary Llerena, Guillermo Pino, José Retuerto, Fernando |
| spellingShingle |
Guzmán, Luis López, Rosmary Llerena, Guillermo Pino, José Retuerto, Fernando Male germinal epithelium recovery in mice treated with only dose of Busulfan |
| author_facet |
Guzmán, Luis López, Rosmary Llerena, Guillermo Pino, José Retuerto, Fernando |
| author_sort |
Guzmán, Luis |
| title |
Male germinal epithelium recovery in mice treated with only dose of Busulfan |
| title_short |
Male germinal epithelium recovery in mice treated with only dose of Busulfan |
| title_full |
Male germinal epithelium recovery in mice treated with only dose of Busulfan |
| title_fullStr |
Male germinal epithelium recovery in mice treated with only dose of Busulfan |
| title_full_unstemmed |
Male germinal epithelium recovery in mice treated with only dose of Busulfan |
| title_sort |
male germinal epithelium recovery in mice treated with only dose of busulfan |
| description |
The DNA replication is disrupted by Busulfan which is a bifunctional alquilant as an ester agent. High dose of this compound are applied before transplants of bone marrow and germ cells. However quantification of the damage to the reproductive system and its recovery have not been established. The aim of this study was to quantify both spermatogenesis and spermiogenesis recovery in mice from damage caused by an injection of Busulfan. We used Balb C mice which were evaluated 30, 54 and 72 days after the intraperitoneal injection of 40 mg/kg body weight. We took the following variables: testicular weight, spermatic concentration in epididimus line and histological cuts to measure the diameter of the seminiferous tubules. Results were analyzed with the test U Mann-Whitney p>0.05. By day 30, testicular weight, spermatic concentration and diameter of the seminiferous tubules were reduced to 77,8%, 97,9% and 34,3% respectively compared to the controls. At day 54, testicular weight and diameter of the seminiferous tubules showed a recovery of 70%. Recovery was complete by day 72. Therefore Busulfan applied in a sigle dose, affects significantly spermatogenesis and the completed recovery takes two spermatic cycles in mice. |
| publisher |
Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas |
| publishDate |
2005 |
| url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2368 |
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