Bioactives Actinomycetes of marine sediment from the central coast of Peru

Bioactives Actinomycetes of marine sediment from the central coast of Peru

In the present research we evaluated the antibacterial and antifungical activity of marine actinomycetes over pathogen of clinical origin. Likewise, it was evaluated the capacity to produce extracellular enzymes like carbohidrases, lipases and proteases. The Actinomycetes were isolated from sediments collected between September to December 2005 of Ancón (Lima) and Independencia (Ica) Bays at depths of 34 and 100 m. The isolation was performed in Casein - Starch Agar (CSA) and Marine Agar (AM) with addition of Cicloheximide (10 μg/mL). The antimicrobial evaluations were done comparing them with pathogenic antibiotic-multiresistant bacteria and fungi from clinical origin; in as much, to evaluate their multienzimatic activity several polimeric substrates were used. A total of 62 actinomycetes were isolated, 31 of there (50%) showed antibacterial activity in opposite to Staphylococcus aureus, 36 (59%) in opposite to Pseudomonas aeruginosa and 23 (37%) to both pathogens. Strains of actinomycetes I-400A and M10-77 identified in each case like Streptomyces and Thermoactinomyces exhibited higher inhibitory activity against S. aureus and P. aeruginosa respectively. Also, 13 actinomycetes (20,97%) showed to antifungical activity against cultures of Candida albicans strain 1511 and 17 (27,42%) with Candida albicans strain 1511MIC; nevertheless, no actinomycete displayed inhibitory activity to the growth of Aspergillus niger, Aspergillus fumigatus and Trichophyton mentagrophytes. Most Actinomycetes showed to have multienzymic activity able to hydrolysis polymerics compounds like the tween-80 (96%), gelatin (95%), starch (93%), lecitine (88%) and casein (74%). Extracts of the active compound obtained from the strain M10-77 with ethyl acetate produced a notable inhibitory activity against S. aureus. We conclude that the marine sediment is source of Actinomycetes with great capacity to produce bioactives substances of medical-pharmacological and industrial relevance.

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Main Authors: León, Jorge, Liza, Libia, Soto, Isela, Cuadra, D´Lourdes, Patiño, Lilian, Zerpa, Rito
Format: Digital revista
Language:spa
Published: Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas 2007
Online Access:https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/1796
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record_format ojs
institution UNMSM
collection OJS
country Perú
countrycode PE
component Revista
access En linea
databasecode rev-rpb-pe
tag revista
region America del Sur
libraryname Biblioteca de la Facultad de Ciencias Biológicas
language spa
format Digital
author León, Jorge
Liza, Libia
Soto, Isela
Cuadra, D´Lourdes
Patiño, Lilian
Zerpa, Rito
spellingShingle León, Jorge
Liza, Libia
Soto, Isela
Cuadra, D´Lourdes
Patiño, Lilian
Zerpa, Rito
Bioactives Actinomycetes of marine sediment from the central coast of Peru
author_facet León, Jorge
Liza, Libia
Soto, Isela
Cuadra, D´Lourdes
Patiño, Lilian
Zerpa, Rito
author_sort León, Jorge
title Bioactives Actinomycetes of marine sediment from the central coast of Peru
title_short Bioactives Actinomycetes of marine sediment from the central coast of Peru
title_full Bioactives Actinomycetes of marine sediment from the central coast of Peru
title_fullStr Bioactives Actinomycetes of marine sediment from the central coast of Peru
title_full_unstemmed Bioactives Actinomycetes of marine sediment from the central coast of Peru
title_sort bioactives actinomycetes of marine sediment from the central coast of peru
description In the present research we evaluated the antibacterial and antifungical activity of marine actinomycetes over pathogen of clinical origin. Likewise, it was evaluated the capacity to produce extracellular enzymes like carbohidrases, lipases and proteases. The Actinomycetes were isolated from sediments collected between September to December 2005 of Ancón (Lima) and Independencia (Ica) Bays at depths of 34 and 100 m. The isolation was performed in Casein - Starch Agar (CSA) and Marine Agar (AM) with addition of Cicloheximide (10 μg/mL). The antimicrobial evaluations were done comparing them with pathogenic antibiotic-multiresistant bacteria and fungi from clinical origin; in as much, to evaluate their multienzimatic activity several polimeric substrates were used. A total of 62 actinomycetes were isolated, 31 of there (50%) showed antibacterial activity in opposite to Staphylococcus aureus, 36 (59%) in opposite to Pseudomonas aeruginosa and 23 (37%) to both pathogens. Strains of actinomycetes I-400A and M10-77 identified in each case like Streptomyces and Thermoactinomyces exhibited higher inhibitory activity against S. aureus and P. aeruginosa respectively. Also, 13 actinomycetes (20,97%) showed to antifungical activity against cultures of Candida albicans strain 1511 and 17 (27,42%) with Candida albicans strain 1511MIC; nevertheless, no actinomycete displayed inhibitory activity to the growth of Aspergillus niger, Aspergillus fumigatus and Trichophyton mentagrophytes. Most Actinomycetes showed to have multienzymic activity able to hydrolysis polymerics compounds like the tween-80 (96%), gelatin (95%), starch (93%), lecitine (88%) and casein (74%). Extracts of the active compound obtained from the strain M10-77 with ethyl acetate produced a notable inhibitory activity against S. aureus. We conclude that the marine sediment is source of Actinomycetes with great capacity to produce bioactives substances of medical-pharmacological and industrial relevance.
publisher Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
publishDate 2007
url https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/1796
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spelling oai:ojs.csi.unmsm:article17962020-05-22T17:56:29Z Bioactives Actinomycetes of marine sediment from the central coast of Peru Actinomycetes bioactivos de sedimento marino de la costa central del Perú León, Jorge Liza, Libia Soto, Isela Cuadra, D´Lourdes Patiño, Lilian Zerpa, Rito Sedimento marino actinomycetes patógenos multirresistentes antibiosis. Marine sediment actinomycetes multiresistant pathogens antibiosis. In the present research we evaluated the antibacterial and antifungical activity of marine actinomycetes over pathogen of clinical origin. Likewise, it was evaluated the capacity to produce extracellular enzymes like carbohidrases, lipases and proteases. The Actinomycetes were isolated from sediments collected between September to December 2005 of Ancón (Lima) and Independencia (Ica) Bays at depths of 34 and 100 m. The isolation was performed in Casein - Starch Agar (CSA) and Marine Agar (AM) with addition of Cicloheximide (10 μg/mL). The antimicrobial evaluations were done comparing them with pathogenic antibiotic-multiresistant bacteria and fungi from clinical origin; in as much, to evaluate their multienzimatic activity several polimeric substrates were used. A total of 62 actinomycetes were isolated, 31 of there (50%) showed antibacterial activity in opposite to Staphylococcus aureus, 36 (59%) in opposite to Pseudomonas aeruginosa and 23 (37%) to both pathogens. Strains of actinomycetes I-400A and M10-77 identified in each case like Streptomyces and Thermoactinomyces exhibited higher inhibitory activity against S. aureus and P. aeruginosa respectively. Also, 13 actinomycetes (20,97%) showed to antifungical activity against cultures of Candida albicans strain 1511 and 17 (27,42%) with Candida albicans strain 1511MIC; nevertheless, no actinomycete displayed inhibitory activity to the growth of Aspergillus niger, Aspergillus fumigatus and Trichophyton mentagrophytes. Most Actinomycetes showed to have multienzymic activity able to hydrolysis polymerics compounds like the tween-80 (96%), gelatin (95%), starch (93%), lecitine (88%) and casein (74%). Extracts of the active compound obtained from the strain M10-77 with ethyl acetate produced a notable inhibitory activity against S. aureus. We conclude that the marine sediment is source of Actinomycetes with great capacity to produce bioactives substances of medical-pharmacological and industrial relevance. En el presente trabajo evaluamos la actividad antibacteriana y antifúngica de actinomycetes marinos sobre patógenos de origen clínico. Asimismo, fueron evaluadas la capacidad de producir enzimas extracelulares como carbohidrasas, lipasas y proteasas. Los Actinomycetes fueron aislados de sedimentos colectados entre setiembre a diciembre del 2005 de las Bahías de Ancón (Lima) e Independencia (Ica) de 34 y 100 m de profundidad. El aislamiento se realizó en Agar Caseína - Almidón (ACA) y Agar Marino (AM) con adición de Cicloheximide (10 μg/mL). Las evaluaciones antimicrobianas fueron realizadas frente a bacterias patógenas antibiótico-multirresistentes y hongos de origen clínico; en tanto, para evaluar su actividad multienzimática se utilizaron sustratos poliméricos diversos. Se aislaron un total de 62 actinomycetes, de los cuales 31 (50%) mostraron actividad antibacteriana frente a Staphylococcus aureus, 36 (59%) frente a Pseudomonas aeruginosa y 23 (37%) a ambos patógenos. Las cepas de actinomycetes I-400A y M10-77 identificadas en cada caso como Streptomyces y Thermoactinomyces fueron las que exhibieron mayor actividad inhibitoria frente a P. aeruginosa y S. aureus respectivamente. Asimismo, 13 actinomycetes (20,97%) mostraron actividad antifúngica frente a cultivos de Candida albicans cepa 1511 y 17 (27,42%) frente a Candida albicans cepa 1511MIC; sin embargo, ningún actinomycete presentó actividad inhibitoria frente a Aspergillus niger, Aspergillus fumigatus y Trichophyton mentagrophytes. La mayoría de los actinomycetes mostraron tener actividad multienzimática capaz de hidrolizar compuestos poliméricos como el tween-80 (96%), la gelatina (95%), almidón (93%), lecitina (88%) y la caseína (74%). Extractos del compuesto activo obtenidos de la cepa M10-77 con etil acetato rindieron notable actividad inhibitoria contra S. aureus. Se concluye que el sedimento marino es fuente de Actinomycetes con gran capacidad de producir sustancias bioactivas de importancia médica-farmacológica e industrial. Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas 2007-12-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article info:eu-repo/semantics/article application/pdf https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/1796 10.15381/rpb.v14i2.1796 Revista Peruana de Biología; Vol. 14 No. 2 (2007); 259-270 Revista Peruana de Biología; Vol. 14 Núm. 2 (2007); 259-270 1727-9933 1561-0837 spa https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/1796/1568 Derechos de autor 2007 Jorge León, Libia Liza, Isela Soto, D´Lourdes Cuadra, Lilian Patiño, Rito Zerpa https://creativecommons.org/licenses/by-nc-sa/4.0

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