Molecular identification of Pichia guillermondii isolated from mine water acidic of Peru and its resistance to heavy metals

Molecular identification of Pichia guillermondii isolated from mine water acidic of Peru and its resistance to heavy metals

Microbe eukaryotes like fungi and protista are common in the drainage of mines. Few information is known on microbial diversity of acid drainages of Peruvian mines. In this work we isolated and characterized the 1M9 yeast. We utilized LSU D1/D2 of the 26S rRNA gene sequence phylogenetic analyses to characterize the diversity the yeast 1MA9 isolated. The strain 1MA9 was closely related to the Pichia guilliermondii. The yeast showed high resistance to Mn2+ (>400 mM), intermediate to ions Zn2+ and Co2+ (400—600 mM), but sensible to ions Cu2+. This work provides the first data on yeasts from an aquatic acid environment of Peruvian mines.

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Main Authors: Orbegozo, Jeanette, Abanto, Michel, García, Ruth, Ramírez, Pablo
Format: Digital revista
Language:spa
Published: Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas 2008
Online Access:https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/1681
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spelling oai:ojs.csi.unmsm:article16812020-05-22T16:55:15Z Molecular identification of Pichia guillermondii isolated from mine water acidic of Peru and its resistance to heavy metals Identificación molecular de Pichia guillermondii aislada de aguas ácidas de minas en el Perú y su resistencia a metales pesados Orbegozo, Jeanette Abanto, Michel García, Ruth Ramírez, Pablo Pichia guilliermondii LSU D1/D2 agua ácida de mina (AMD) resistencia a metales levaduras Pichia guilliermondii LSU D1/D2 acid mine drainage Heavy metals resistance yeast Microbe eukaryotes like fungi and protista are common in the drainage of mines. Few information is known on microbial diversity of acid drainages of Peruvian mines. In this work we isolated and characterized the 1M9 yeast. We utilized LSU D1/D2 of the 26S rRNA gene sequence phylogenetic analyses to characterize the diversity the yeast 1MA9 isolated. The strain 1MA9 was closely related to the Pichia guilliermondii. The yeast showed high resistance to Mn2+ (>400 mM), intermediate to ions Zn2+ and Co2+ (400—600 mM), but sensible to ions Cu2+. This work provides the first data on yeasts from an aquatic acid environment of Peruvian mines. Las aguas ácidas de minas tienen una considerable diversidad de microorganismos eucariontes, entre ellos hongos y protistas; en particular, poco se conoce sobre la diversidad de levaduras en drenajes ácidos de minas peruanas. En el presente estudio se aisló y caracterizó la levadura 1MA9, mediante la amplificación y secuenciación del LSU D1/D2 del gen rRNA 26S. La cepa 1MA9 se identificó molecularmente como Pichia guillermondi, ésta presentó una resistencia alta a Mn2+ (>400 mM), intermedia a iones Zn2+ y Co2+ (400—600 mM), pero fue sensible a iones Cu2+. Este trabajo representa el primer reporte de levaduras en ambientes acuáticos ácidos proveniente de minas peruanas. Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas 2008-04-14 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article info:eu-repo/semantics/article application/pdf https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/1681 10.15381/rpb.v15i1.1681 Revista Peruana de Biología; Vol. 15 No. 1 (2008); 91-96 Revista Peruana de Biología; Vol. 15 Núm. 1 (2008); 91-96 1727-9933 1561-0837 spa https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/1681/1456 Derechos de autor 2008 Jeanette Orbegozo, Michel Abanto, Ruth García, Pablo Ramírez https://creativecommons.org/licenses/by-nc-sa/4.0
institution UNMSM
collection OJS
country Perú
countrycode PE
component Revista
access En linea
databasecode rev-rpb-pe
tag revista
region America del Sur
libraryname Biblioteca de la Facultad de Ciencias Biológicas
language spa
format Digital
author Orbegozo, Jeanette
Abanto, Michel
García, Ruth
Ramírez, Pablo
spellingShingle Orbegozo, Jeanette
Abanto, Michel
García, Ruth
Ramírez, Pablo
Molecular identification of Pichia guillermondii isolated from mine water acidic of Peru and its resistance to heavy metals
author_facet Orbegozo, Jeanette
Abanto, Michel
García, Ruth
Ramírez, Pablo
author_sort Orbegozo, Jeanette
title Molecular identification of Pichia guillermondii isolated from mine water acidic of Peru and its resistance to heavy metals
title_short Molecular identification of Pichia guillermondii isolated from mine water acidic of Peru and its resistance to heavy metals
title_full Molecular identification of Pichia guillermondii isolated from mine water acidic of Peru and its resistance to heavy metals
title_fullStr Molecular identification of Pichia guillermondii isolated from mine water acidic of Peru and its resistance to heavy metals
title_full_unstemmed Molecular identification of Pichia guillermondii isolated from mine water acidic of Peru and its resistance to heavy metals
title_sort molecular identification of pichia guillermondii isolated from mine water acidic of peru and its resistance to heavy metals
description Microbe eukaryotes like fungi and protista are common in the drainage of mines. Few information is known on microbial diversity of acid drainages of Peruvian mines. In this work we isolated and characterized the 1M9 yeast. We utilized LSU D1/D2 of the 26S rRNA gene sequence phylogenetic analyses to characterize the diversity the yeast 1MA9 isolated. The strain 1MA9 was closely related to the Pichia guilliermondii. The yeast showed high resistance to Mn2+ (>400 mM), intermediate to ions Zn2+ and Co2+ (400—600 mM), but sensible to ions Cu2+. This work provides the first data on yeasts from an aquatic acid environment of Peruvian mines.
publisher Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
publishDate 2008
url https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/1681
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