Identification and assessment of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pigs (cavia porcellus)

Identification and assessment of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pigs (cavia porcellus)

The aim of this study was to identify and assess the presence of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pig (Cavia porcellus). Five animals of 90 days old were used. Samples from the duodenum, jejunum and ileum were collected after slaughtering to carry out immunohistochemistry assays. Expression of SGLT1, GLUT2 and GLP-1 was observed in all three portions of the small intestine, where SGLT1 showed the highest expression, with a strong staining intensity in the apical and medium section of the crypt-villus axis. GLUT2 showed a strong staining intensity throughout the crypt-villus axis in the jejunum. GLP-1 expression was indirectly analyzed by calculating the number of L cells per mm2 of intestinal mucosa, resulting that the jejunum had the largest number (8.46 ±1.16 cells/mm2) (p<0.05). The results suggest that SGLT1 glucose transporter would be the most important in the guinea pig, and unlike other species, GLP-1 may be mostly secreted by the jejunum.

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Main Authors: Anglas P., Juan, Cueva M., Sergio, Vásquez C., María, Lira M., Boris, Espinoza B., Juan, Lucas L., Juan, Rodríguez G., José
Format: Digital revista
Language:spa
Published: Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2012
Online Access:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/932
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spelling oai:ojs.csi.unmsm:article-9322020-03-20T01:57:56Z Identification and assessment of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pigs (cavia porcellus) IDENTIFICACIÓN Y EVALUACIÓN DE TRANSPORTADORES DE GLUCOSA SGLT1 Y GLUT2 Y LA INCRETINA GLP-1 EN INTESTINO DELGADO DE CUYES (Cavia porcellus). Anglas P., Juan Cueva M., Sergio Vásquez C., María Lira M., Boris Espinoza B., Juan Lucas L., Juan Rodríguez G., José SGLT1 GLUT2 GLP-1 intestino delgado cuyes SGLT1 GLUT2 GLP-1 small intestine guinea pigs The aim of this study was to identify and assess the presence of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pig (Cavia porcellus). Five animals of 90 days old were used. Samples from the duodenum, jejunum and ileum were collected after slaughtering to carry out immunohistochemistry assays. Expression of SGLT1, GLUT2 and GLP-1 was observed in all three portions of the small intestine, where SGLT1 showed the highest expression, with a strong staining intensity in the apical and medium section of the crypt-villus axis. GLUT2 showed a strong staining intensity throughout the crypt-villus axis in the jejunum. GLP-1 expression was indirectly analyzed by calculating the number of L cells per mm2 of intestinal mucosa, resulting that the jejunum had the largest number (8.46 ±1.16 cells/mm2) (p<0.05). The results suggest that SGLT1 glucose transporter would be the most important in the guinea pig, and unlike other species, GLP-1 may be mostly secreted by the jejunum. El presente trabajo tuvo por objetivo identificar y evaluar la presencia de los transportadores de glucosa SGLT1 y GLUT2 y la incretina GLP-1 en intestino delgado de cuyes. Se usaron cinco cuyes (Cavia porcellus) reproductores de 90 días de edad. Luego del sacrificio, se tomaron muestras de duodeno, yeyuno e íleon para el procesamiento inmunohistoquímico. Se pudo identificar la presencia de SGLT1, GLUT2 y GLP-1 en las tres porciones intestinales, siendo SGLT1 la proteína de mayor presencia, con un nivel de marcación fuerte en la parte apical y media del eje cripta-vellosidad. El GLUT2 mostró un nivel de marcación fuerte en todo el eje cripta-vellosidad en el yeyuno. Asimismo, la presencia del GLP-1 fue analizada indirectamente por el número de células L/mm2 de mucosa intestinal, encontrándose el mayor número en el yeyuno (8.46 ± 1.16 células L/mm2) (p<0.05). Los resultados indican que SGLT1 sería el transportador de glucosa más importante en el cuy y, que a diferencia de otras especies, la mayor secreción de GLP-1 sería en yeyuno. Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2012-12-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article application/pdf https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/932 10.15381/rivep.v23i4.932 Revista de Investigaciones Veterinarias del Perú; Vol. 23 No. 4 (2012); 399-405 Revista de Investigaciones Veterinarias del Perú; Vol. 23 Núm. 4 (2012); 399-405 1682-3419 1609-9117 spa https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/932/757 Derechos de autor 2012 Juan Anglas P., Sergio Cueva M., María Vásquez C., Boris Lira M., Juan Espinoza B., Juan Lucas L., José Rodríguez G. https://creativecommons.org/licenses/by-nc-sa/4.0
institution UNMSM
collection OJS
country Perú
countrycode PE
component Revista
access En linea
databasecode rev-rivep
tag revista
region America del Sur
libraryname Biblioteca de la Facultad de Medicina Veterinaria
language spa
format Digital
author Anglas P., Juan
Cueva M., Sergio
Vásquez C., María
Lira M., Boris
Espinoza B., Juan
Lucas L., Juan
Rodríguez G., José
spellingShingle Anglas P., Juan
Cueva M., Sergio
Vásquez C., María
Lira M., Boris
Espinoza B., Juan
Lucas L., Juan
Rodríguez G., José
Identification and assessment of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pigs (cavia porcellus)
author_facet Anglas P., Juan
Cueva M., Sergio
Vásquez C., María
Lira M., Boris
Espinoza B., Juan
Lucas L., Juan
Rodríguez G., José
author_sort Anglas P., Juan
title Identification and assessment of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pigs (cavia porcellus)
title_short Identification and assessment of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pigs (cavia porcellus)
title_full Identification and assessment of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pigs (cavia porcellus)
title_fullStr Identification and assessment of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pigs (cavia porcellus)
title_full_unstemmed Identification and assessment of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pigs (cavia porcellus)
title_sort identification and assessment of glucose transporters sglt1 and glut2 and incretin glp-1 in the small intestine of guinea pigs (cavia porcellus)
description The aim of this study was to identify and assess the presence of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pig (Cavia porcellus). Five animals of 90 days old were used. Samples from the duodenum, jejunum and ileum were collected after slaughtering to carry out immunohistochemistry assays. Expression of SGLT1, GLUT2 and GLP-1 was observed in all three portions of the small intestine, where SGLT1 showed the highest expression, with a strong staining intensity in the apical and medium section of the crypt-villus axis. GLUT2 showed a strong staining intensity throughout the crypt-villus axis in the jejunum. GLP-1 expression was indirectly analyzed by calculating the number of L cells per mm2 of intestinal mucosa, resulting that the jejunum had the largest number (8.46 ±1.16 cells/mm2) (p<0.05). The results suggest that SGLT1 glucose transporter would be the most important in the guinea pig, and unlike other species, GLP-1 may be mostly secreted by the jejunum.
publisher Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publishDate 2012
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/932
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