Neonatal enteric complex in andean alpacas
Advances in research on the major causative agents of morbidity and mortality in newborn alpacas associated with enteric processes in southern Peru were reviewed. Microbiology and molecular analyses performed on intestinal samples from enterotoxemia fatalities confirmed the predominance of C. perfringens type A carrying only the gene coding for the major α exotoxin and identifiying for the first time the presence of the novel β2 toxin gene. In vitro studies have yielded three profiles for phospholipase activity (high, medium and low) with biological activity when high and medium strains were inoculated intraintestinally in mice and rabbits, but did not induce intestinal pathology in an alpaca cria. A detailed histopathological investigation has reveled that within necrotizing hemorrhagic enteritis Clostridium coexist with massive presence of Eimeria macusaniensis suggesting that primary parasite tissue destruction may well predispose overgrowth of clostridium and toxin production, triggering enteric fatalities. Additionally, studies on diarrheas intestinal swabs and/or intestinal contents identified Escherichia coli pathogenic strains (enteropathogenic and enterohemorrhagic). The immunofluorescent direct test and PCR revealed the presence of Giardia intestinalis coexisting with mostly enteropathogenic and enterohemorrhagic E. coli strains, as well as with a virus similar to bovine coronavirus. These microbes are potentially diarrheagenic pathogens and a possible infection source for Andean people.
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Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
2012
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Rosadio A., Raúl Maturrano H., Lenin Pérez J., David Luna E., Luis |
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Rosadio A., Raúl Maturrano H., Lenin Pérez J., David Luna E., Luis Neonatal enteric complex in andean alpacas |
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Rosadio A., Raúl Maturrano H., Lenin Pérez J., David Luna E., Luis |
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Rosadio A., Raúl |
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Neonatal enteric complex in andean alpacas |
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Neonatal enteric complex in andean alpacas |
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Neonatal enteric complex in andean alpacas |
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Neonatal enteric complex in andean alpacas |
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Neonatal enteric complex in andean alpacas |
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neonatal enteric complex in andean alpacas |
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Advances in research on the major causative agents of morbidity and mortality in newborn alpacas associated with enteric processes in southern Peru were reviewed. Microbiology and molecular analyses performed on intestinal samples from enterotoxemia fatalities confirmed the predominance of C. perfringens type A carrying only the gene coding for the major α exotoxin and identifiying for the first time the presence of the novel β2 toxin gene. In vitro studies have yielded three profiles for phospholipase activity (high, medium and low) with biological activity when high and medium strains were inoculated intraintestinally in mice and rabbits, but did not induce intestinal pathology in an alpaca cria. A detailed histopathological investigation has reveled that within necrotizing hemorrhagic enteritis Clostridium coexist with massive presence of Eimeria macusaniensis suggesting that primary parasite tissue destruction may well predispose overgrowth of clostridium and toxin production, triggering enteric fatalities. Additionally, studies on diarrheas intestinal swabs and/or intestinal contents identified Escherichia coli pathogenic strains (enteropathogenic and enterohemorrhagic). The immunofluorescent direct test and PCR revealed the presence of Giardia intestinalis coexisting with mostly enteropathogenic and enterohemorrhagic E. coli strains, as well as with a virus similar to bovine coronavirus. These microbes are potentially diarrheagenic pathogens and a possible infection source for Andean people. |
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Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
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2012 |
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https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/908 |
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AT rosadioaraul neonatalentericcomplexinandeanalpacas AT maturranohlenin neonatalentericcomplexinandeanalpacas AT perezjdavid neonatalentericcomplexinandeanalpacas AT lunaeluis neonatalentericcomplexinandeanalpacas AT rosadioaraul elcomplejoentericoneonatalenalpacasandinas AT maturranohlenin elcomplejoentericoneonatalenalpacasandinas AT perezjdavid elcomplejoentericoneonatalenalpacasandinas AT lunaeluis elcomplejoentericoneonatalenalpacasandinas |
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oai:ojs.csi.unmsm:article-9082020-03-20T00:23:45Z Neonatal enteric complex in andean alpacas EL COMPLEJO ENTÉRICO NEONATAL EN ALPACAS ANDINAS. Rosadio A., Raúl Maturrano H., Lenin Pérez J., David Luna E., Luis neonatal enteritis Clostridium perfringens E. coli Eimeria macusaniensis Giardia intestinalis alpacas Enteritis neonatal Clostridium perfringens E. coli Eimeria macusaniensis Giardia intestinalis alpacas. Advances in research on the major causative agents of morbidity and mortality in newborn alpacas associated with enteric processes in southern Peru were reviewed. Microbiology and molecular analyses performed on intestinal samples from enterotoxemia fatalities confirmed the predominance of C. perfringens type A carrying only the gene coding for the major α exotoxin and identifiying for the first time the presence of the novel β2 toxin gene. In vitro studies have yielded three profiles for phospholipase activity (high, medium and low) with biological activity when high and medium strains were inoculated intraintestinally in mice and rabbits, but did not induce intestinal pathology in an alpaca cria. A detailed histopathological investigation has reveled that within necrotizing hemorrhagic enteritis Clostridium coexist with massive presence of Eimeria macusaniensis suggesting that primary parasite tissue destruction may well predispose overgrowth of clostridium and toxin production, triggering enteric fatalities. Additionally, studies on diarrheas intestinal swabs and/or intestinal contents identified Escherichia coli pathogenic strains (enteropathogenic and enterohemorrhagic). The immunofluorescent direct test and PCR revealed the presence of Giardia intestinalis coexisting with mostly enteropathogenic and enterohemorrhagic E. coli strains, as well as with a virus similar to bovine coronavirus. These microbes are potentially diarrheagenic pathogens and a possible infection source for Andean people. Se revisan avances de investigaciones sobre los principales agentes causales de morbilidad y mortalidad en alpacas neonatales asociadas a procesos entéricos en el sur peruano. Análisis microbiológicos confirman al Clostridium perfringens tipo A como el agente prevalente en fatalidades asociadas con enterotoxemia e identifican por primera vez la presencia del gen secundario β2. El análisis in vitro argumenta la existencia de cepas con tres perfiles en la actividad fosfolipídica (alta, mediana y baja), mostrando que las cepas de medianas y altas producciones fueron capaces de producir lesiones intestinales en conejos pero incapaces de producir similares lesiones en crías de alpacas. Estudios histopatológicos revelan la coexistencia de Clostridium y E. macusaniensis en lesiones de enteritis hemorrágica en muestras intestinales de crías muertas por enterotoxemia, sugiriendo a las infecciones coccidiales como posibles agentes desencadenantes de fatalidades conocidas como enterotoxemia. Por otro lado, estudios realizados en hisopados clínicos o contenidos intestinales han identificado molecularmente cepas de E. coli patogénicas (enteropatogénicas y enterohemorrágicas). Adicionalmente, en casos clínicos de diarreas neonatales, mediante inmunofluorescencia directa y PCR, se ha detectado Giardia intestinalis coexistiendo con cepas de E. coli enteropatogénicas, así como un coronavirus similar al virus bovino. Estos patógenos son potenciales causantes de procesos diarreicos en animales incluyendo a poblaciones humanas. Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2012-09-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article application/pdf https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/908 10.15381/rivep.v23i3.908 Revista de Investigaciones Veterinarias del Perú; Vol. 23 No. 3 (2012); 261-271 Revista de Investigaciones Veterinarias del Perú; Vol. 23 Núm. 3 (2012); 261-271 1682-3419 1609-9117 spa https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/908/733 Derechos de autor 2012 Raúl Rosadio A., Lenin Maturrano H., David Pérez J., Luis Luna E. https://creativecommons.org/licenses/by-nc-sa/4.0 |