LECTINHISTOCHEMISTRY STUDY OF THE UTERUS OF ALPACAS (Vicugna pacos)UNDER SUPEROVULATION TREATMENT

LECTINHISTOCHEMISTRY STUDY OF THE UTERUS OF ALPACAS (Vicugna pacos)UNDER SUPEROVULATION TREATMENT

The aim of this study was to determine the glycosylation pattern in the uterine endometrium of alpacas with and without superovulation treatment. Uterine tissues were obtained from five non-treated alpacas with ovarian follicles less than 4 mm and five superovulated alpacas where FSH, GnRH and hCG was used. Samples from theuterus and uterine horns were analyzed by histochemistry (PAS and alcian blue) and lectinhistochemistry techniques. Fourteen lectins were used and PNA, RCA-1, ConA, DBA, WGA, Swga, GS, LCA, SJA and PHA-L showed different glycosilation patterns in the endometrium between both groups, indicating that the hormonal treatment could affect the expression of glycoconjugates that have affinity for these lectins. Lectins PNA, SJA, SBA, ConA, LCA and PSA showed different glycosilation patterns between the two uterine horns, indicating that the glycoconjugates that have affinity for these lectins can be implicated in the uterine environment during the implantation, which in most cases in the alpaca take place on the left horn. In conclusion, the glycosylation pattern in the alpaca can change due to superovulation hormone therapy.

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Main Authors: López C., Verónica, Vásquez C., Maria, Huanca L., Wilfredo, Santiani A., Alexei, Barbeito., Claudio, Canuzzi A., Carolina, Lira M., Boris, Rodríguez G., Jose
Format: Digital revista
Language:spa
Published: Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2014
Online Access:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/8463
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spelling oai:ojs.csi.unmsm:article-84632020-03-29T17:09:35Z LECTINHISTOCHEMISTRY STUDY OF THE UTERUS OF ALPACAS (Vicugna pacos)UNDER SUPEROVULATION TREATMENT ESTUDIO LECTINHISTOQUÍMICO DEL ÚTERO DE ALPACAS (Vicugna pacos) BAJO TRATAMIENTO SUPEROVULATORIO. López C., Verónica Vásquez C., Maria Huanca L., Wilfredo Santiani A., Alexei Barbeito., Claudio Canuzzi A., Carolina Lira M., Boris Rodríguez G., Jose Alpaca lectinas patrón de glicosilación endometrio uterino superovulación. alpaca lectins glycosylation pattern endometrium superovulation The aim of this study was to determine the glycosylation pattern in the uterine endometrium of alpacas with and without superovulation treatment. Uterine tissues were obtained from five non-treated alpacas with ovarian follicles less than 4 mm and five superovulated alpacas where FSH, GnRH and hCG was used. Samples from theuterus and uterine horns were analyzed by histochemistry (PAS and alcian blue) and lectinhistochemistry techniques. Fourteen lectins were used and PNA, RCA-1, ConA, DBA, WGA, Swga, GS, LCA, SJA and PHA-L showed different glycosilation patterns in the endometrium between both groups, indicating that the hormonal treatment could affect the expression of glycoconjugates that have affinity for these lectins. Lectins PNA, SJA, SBA, ConA, LCA and PSA showed different glycosilation patterns between the two uterine horns, indicating that the glycoconjugates that have affinity for these lectins can be implicated in the uterine environment during the implantation, which in most cases in the alpaca take place on the left horn. In conclusion, the glycosylation pattern in the alpaca can change due to superovulation hormone therapy. El objetivo del presente estudio fue determinar el patrón de glicosilación del endometrio uterino en alpacas con y sin tratamiento de superovulación. Se utilizaron cinco alpacas no superovuladas con folículos ováricos menores de 4 mm y cinco alpacas que fueron sometidas a un tratamiento de superovulación en base a FSH, GnRH y hCG. Se tomaron muestras del cuerpo del útero y de ambos cuernos uterinos y se trabajaron con las técnicas histoquímicas PAS y Azul Alcián y con la técnica de lectinhistoquímica. Para esta última se utilizaron 14 lectinas, de las cuales PNA, RCA-I, ConA, DBA, WGA,sWGA, GS, LCA, SJA y PHA-L presentaron un patrón de glicosilación diferente entre ambos grupos, sugiriendo que el tratamiento hormonal influenció en la expresión de los glicoconjugados que se unen a estas lectinas. Asimismo, las lectinas PNA, SJA, SBA,ConA, LCA y PSA presentaron diferencias entre el cuerno derecho y el cuerno izquierdo, lo que sugiere que los glicoconjugados de unión a estas lectinas podrían participar en generar un ambiente propicio para la implantación, que por lo general se da en el cuerno izquierdo en la alpaca. Se concluye que la alpaca tiene un patrón de glicosilación que puede variar con tratamientos hormonales de superovulación. Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2014-03-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article application/pdf https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/8463 10.15381/rivep.v25i1.8463 Revista de Investigaciones Veterinarias del Perú; Vol. 25 No. 1 (2014); 01-15 Revista de Investigaciones Veterinarias del Perú; Vol. 25 Núm. 1 (2014); 01-15 1682-3419 1609-9117 spa https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/8463/7346 Derechos de autor 2014 Verónica López C., Maria Vásquez C., Wilfredo Huanca L., Alexei Santiani A., Claudio Barbeito., Carolina Canuzzi A., Boris Lira M., Jose Rodríguez G. https://creativecommons.org/licenses/by-nc-sa/4.0
institution UNMSM
collection OJS
country Perú
countrycode PE
component Revista
access En linea
databasecode rev-rivep
tag revista
region America del Sur
libraryname Biblioteca de la Facultad de Medicina Veterinaria
language spa
format Digital
author López C., Verónica
Vásquez C., Maria
Huanca L., Wilfredo
Santiani A., Alexei
Barbeito., Claudio
Canuzzi A., Carolina
Lira M., Boris
Rodríguez G., Jose
spellingShingle López C., Verónica
Vásquez C., Maria
Huanca L., Wilfredo
Santiani A., Alexei
Barbeito., Claudio
Canuzzi A., Carolina
Lira M., Boris
Rodríguez G., Jose
LECTINHISTOCHEMISTRY STUDY OF THE UTERUS OF ALPACAS (Vicugna pacos)UNDER SUPEROVULATION TREATMENT
author_facet López C., Verónica
Vásquez C., Maria
Huanca L., Wilfredo
Santiani A., Alexei
Barbeito., Claudio
Canuzzi A., Carolina
Lira M., Boris
Rodríguez G., Jose
author_sort López C., Verónica
title LECTINHISTOCHEMISTRY STUDY OF THE UTERUS OF ALPACAS (Vicugna pacos)UNDER SUPEROVULATION TREATMENT
title_short LECTINHISTOCHEMISTRY STUDY OF THE UTERUS OF ALPACAS (Vicugna pacos)UNDER SUPEROVULATION TREATMENT
title_full LECTINHISTOCHEMISTRY STUDY OF THE UTERUS OF ALPACAS (Vicugna pacos)UNDER SUPEROVULATION TREATMENT
title_fullStr LECTINHISTOCHEMISTRY STUDY OF THE UTERUS OF ALPACAS (Vicugna pacos)UNDER SUPEROVULATION TREATMENT
title_full_unstemmed LECTINHISTOCHEMISTRY STUDY OF THE UTERUS OF ALPACAS (Vicugna pacos)UNDER SUPEROVULATION TREATMENT
title_sort lectinhistochemistry study of the uterus of alpacas (vicugna pacos)under superovulation treatment
description The aim of this study was to determine the glycosylation pattern in the uterine endometrium of alpacas with and without superovulation treatment. Uterine tissues were obtained from five non-treated alpacas with ovarian follicles less than 4 mm and five superovulated alpacas where FSH, GnRH and hCG was used. Samples from theuterus and uterine horns were analyzed by histochemistry (PAS and alcian blue) and lectinhistochemistry techniques. Fourteen lectins were used and PNA, RCA-1, ConA, DBA, WGA, Swga, GS, LCA, SJA and PHA-L showed different glycosilation patterns in the endometrium between both groups, indicating that the hormonal treatment could affect the expression of glycoconjugates that have affinity for these lectins. Lectins PNA, SJA, SBA, ConA, LCA and PSA showed different glycosilation patterns between the two uterine horns, indicating that the glycoconjugates that have affinity for these lectins can be implicated in the uterine environment during the implantation, which in most cases in the alpaca take place on the left horn. In conclusion, the glycosylation pattern in the alpaca can change due to superovulation hormone therapy.
publisher Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publishDate 2014
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/8463
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