Macroscopic description of the fetal ossification pattern in alpacas (Vicugna pacos)

Knowledge of embryonic and foetal development is extremely important in order to establish normal development parameters, the health of the foetus, and to identify critical periods in the growth curve. One of these processes is the pattern of ossification. The present study describes the ossification pattern in alpaca embryos and foetuses using radiology and diaphanization techniques. Seventeen pregnant female alpacas were used for the collection of embryos and foetuses of various ages (32, 45, 60, 90, 118, 150, 165, 190, 220 and 280 days). The specimens were weighed, measured and radiographed, and later fixed in 10% buffered formalin to be subjected to the diaphanization process in potassium hydroxide and finally stained with Alizarin Red. The description of the specimens indicates that in embryos of 32 and 45 days there are no mineralized structures; however, it can be seen radiopaque structures and signs of mineralization at 60 days, indicating that ossification would be starting between 45 and 60 days of gestation. The bones of the skull and the rib cage are the first to ossify. Ossification occurs consecutively in the vertebral column, thoracic and pelvic limbs, reaching an advanced state of ossification in the last third of gestation.

Saved in:
Bibliographic Details
Main Authors: Iván Pacheco, Joel, Manuel Velez, Víctor, Garcia, Wilber, Vasquez-Cachay, María, Zapata, Celso, Lombardo, Daniel
Format: Digital revista
Language:spa
Published: Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2023
Online Access:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/25101
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:ojs.csi.unmsm:article-25101
record_format ojs
spelling oai:ojs.csi.unmsm:article-251012023-05-02T17:02:49Z Macroscopic description of the fetal ossification pattern in alpacas (Vicugna pacos) Descripción macroscópica del patrón de osificación fetal en alpacas (Vicugna pacos) Iván Pacheco, Joel Manuel Velez, Víctor Garcia, Wilber Vasquez-Cachay, María Zapata, Celso Lombardo, Daniel ossification gestation alpacas diaphanization osificación gestación alpacas diafanización Knowledge of embryonic and foetal development is extremely important in order to establish normal development parameters, the health of the foetus, and to identify critical periods in the growth curve. One of these processes is the pattern of ossification. The present study describes the ossification pattern in alpaca embryos and foetuses using radiology and diaphanization techniques. Seventeen pregnant female alpacas were used for the collection of embryos and foetuses of various ages (32, 45, 60, 90, 118, 150, 165, 190, 220 and 280 days). The specimens were weighed, measured and radiographed, and later fixed in 10% buffered formalin to be subjected to the diaphanization process in potassium hydroxide and finally stained with Alizarin Red. The description of the specimens indicates that in embryos of 32 and 45 days there are no mineralized structures; however, it can be seen radiopaque structures and signs of mineralization at 60 days, indicating that ossification would be starting between 45 and 60 days of gestation. The bones of the skull and the rib cage are the first to ossify. Ossification occurs consecutively in the vertebral column, thoracic and pelvic limbs, reaching an advanced state of ossification in the last third of gestation. El conocimiento del desarrollo embrionario y fetal es de suma importancia para poder establecer parámetros de desarrollo normal, la salud del feto y para identificar periodos críticos en la curva de crecimiento. Uno de estos procesos es el patrón de osificación. El presente estudio describe el patrón de osificación en embriones y fetos de alpaca utilizando las técnicas de radiología y diafanización. Se utilizaron 17 alpacas hembra preñadas para la colección de embriones y fetos de variada edad (32, 45, 60, 90, 118, 150, 165, 190, 220 y 280 días). Los especímenes fueron pesados, medidos y radiografiados, y posteriormente fijados en formol tamponado al 10% para ser sometidos al proceso de diafanización en hidróxido de potasio y finalmente teñidos con Rojo de Alizarina. La descripción de los especímenes indica que en embriones de 32 y 45 días no existen estructuras mineralizadas; sin embargo, se pueden ver estructuras radiopacas e indicios de mineralización a los 60 días, indicando que la osificación se estaría iniciando entre los 45 y 60 días de gestación. Los huesos del cráneo y de la caja torácica son los primeros en osificarse. La osificación se presenta de manera consecutiva en la columna vertebral, miembros torácicos y pélvicos, llegando a un estado avanzado de osificación en el último tercio de gestación. Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2023-04-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article application/pdf https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/25101 10.15381/rivep.v34i2.25101 Revista de Investigaciones Veterinarias del Perú; Vol. 34 No. 2 (2023); e25101 Revista de Investigaciones Veterinarias del Perú; Vol. 34 Núm. 2 (2023); e25101 1682-3419 1609-9117 spa https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/25101/19465 Derechos de autor 2023 Joel Iván Pacheco, Víctor Manuel Velez, Wilber Garcia, María Vasquez-Cachay, Celso Zapata, Daniel Lombardo http://creativecommons.org/licenses/by/4.0
institution UNMSM
collection OJS
country Perú
countrycode PE
component Revista
access En linea
databasecode rev-rivep
tag revista
region America del Sur
libraryname Biblioteca de la Facultad de Medicina Veterinaria
language spa
format Digital
author Iván Pacheco, Joel
Manuel Velez, Víctor
Garcia, Wilber
Vasquez-Cachay, María
Zapata, Celso
Lombardo, Daniel
spellingShingle Iván Pacheco, Joel
Manuel Velez, Víctor
Garcia, Wilber
Vasquez-Cachay, María
Zapata, Celso
Lombardo, Daniel
Macroscopic description of the fetal ossification pattern in alpacas (Vicugna pacos)
author_facet Iván Pacheco, Joel
Manuel Velez, Víctor
Garcia, Wilber
Vasquez-Cachay, María
Zapata, Celso
Lombardo, Daniel
author_sort Iván Pacheco, Joel
title Macroscopic description of the fetal ossification pattern in alpacas (Vicugna pacos)
title_short Macroscopic description of the fetal ossification pattern in alpacas (Vicugna pacos)
title_full Macroscopic description of the fetal ossification pattern in alpacas (Vicugna pacos)
title_fullStr Macroscopic description of the fetal ossification pattern in alpacas (Vicugna pacos)
title_full_unstemmed Macroscopic description of the fetal ossification pattern in alpacas (Vicugna pacos)
title_sort macroscopic description of the fetal ossification pattern in alpacas (vicugna pacos)
description Knowledge of embryonic and foetal development is extremely important in order to establish normal development parameters, the health of the foetus, and to identify critical periods in the growth curve. One of these processes is the pattern of ossification. The present study describes the ossification pattern in alpaca embryos and foetuses using radiology and diaphanization techniques. Seventeen pregnant female alpacas were used for the collection of embryos and foetuses of various ages (32, 45, 60, 90, 118, 150, 165, 190, 220 and 280 days). The specimens were weighed, measured and radiographed, and later fixed in 10% buffered formalin to be subjected to the diaphanization process in potassium hydroxide and finally stained with Alizarin Red. The description of the specimens indicates that in embryos of 32 and 45 days there are no mineralized structures; however, it can be seen radiopaque structures and signs of mineralization at 60 days, indicating that ossification would be starting between 45 and 60 days of gestation. The bones of the skull and the rib cage are the first to ossify. Ossification occurs consecutively in the vertebral column, thoracic and pelvic limbs, reaching an advanced state of ossification in the last third of gestation.
publisher Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publishDate 2023
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/25101
work_keys_str_mv AT ivanpachecojoel macroscopicdescriptionofthefetalossificationpatterninalpacasvicugnapacos
AT manuelvelezvictor macroscopicdescriptionofthefetalossificationpatterninalpacasvicugnapacos
AT garciawilber macroscopicdescriptionofthefetalossificationpatterninalpacasvicugnapacos
AT vasquezcachaymaria macroscopicdescriptionofthefetalossificationpatterninalpacasvicugnapacos
AT zapatacelso macroscopicdescriptionofthefetalossificationpatterninalpacasvicugnapacos
AT lombardodaniel macroscopicdescriptionofthefetalossificationpatterninalpacasvicugnapacos
AT ivanpachecojoel descripcionmacroscopicadelpatrondeosificacionfetalenalpacasvicugnapacos
AT manuelvelezvictor descripcionmacroscopicadelpatrondeosificacionfetalenalpacasvicugnapacos
AT garciawilber descripcionmacroscopicadelpatrondeosificacionfetalenalpacasvicugnapacos
AT vasquezcachaymaria descripcionmacroscopicadelpatrondeosificacionfetalenalpacasvicugnapacos
AT zapatacelso descripcionmacroscopicadelpatrondeosificacionfetalenalpacasvicugnapacos
AT lombardodaniel descripcionmacroscopicadelpatrondeosificacionfetalenalpacasvicugnapacos
_version_ 1770194614935879680