Repeatability of the superovulatory response in embryo production in vivo with a modified pFSH protocol in Australian Merino sheep
The study evaluated the repeatability of the superovulatory response in 12 Australian Merino ewes during the breeding season in one (G1), three (G2), and four (G3) consecutive years. A modified protocol was used based on 200 mg pFSH in eight decreasing doses (12-h intervals) and supportive strategies such as changing the CIDR at day 7, spraying the horns with heparinized saline during the procedure, and use of Flunixin Meglumine. No significant differences were found in the total number of structures recovered during the years of application of the superovulation protocol (G1=8±2, G2=18±3, G3=19±4; p>0.05). High repeatability was observed, both for the total number of embryos and for the number of transferable embryos in G2 and G3. Superovulatory treatment for 3 and 4 successive years did not affect the efficacy of embryo retrieval.
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Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
2023
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oai:ojs.csi.unmsm:article-246042023-12-18T05:17:52Z Repeatability of the superovulatory response in embryo production in vivo with a modified pFSH protocol in Australian Merino sheep Repetibilidad de la respuesta superovulatoria en la producción de embriones in vivo con un protocolo modificado de pFSH en ovejas Merino Australiano Gómez-Ceruti, Marcela Serrano, Carlos Mariano Catenacci A., Giordano Correa E., Lina Maria Letelier-Velasquez, Claudia sheep superovulation FSH embryos surgical recovery Oveja Superovulación FSH Embriones Recuperación quirúrgica The study evaluated the repeatability of the superovulatory response in 12 Australian Merino ewes during the breeding season in one (G1), three (G2), and four (G3) consecutive years. A modified protocol was used based on 200 mg pFSH in eight decreasing doses (12-h intervals) and supportive strategies such as changing the CIDR at day 7, spraying the horns with heparinized saline during the procedure, and use of Flunixin Meglumine. No significant differences were found in the total number of structures recovered during the years of application of the superovulation protocol (G1=8±2, G2=18±3, G3=19±4; p>0.05). High repeatability was observed, both for the total number of embryos and for the number of transferable embryos in G2 and G3. Superovulatory treatment for 3 and 4 successive years did not affect the efficacy of embryo retrieval. El estudio evaluó la repetibilidad de la respuesta superovulatoria en 12 ovejas Merino Australiano durante la estación reproductiva en uno (G1), tres (G2) y cuatro (G3) años consecutivos. Se utilizó un protocolo modificado basado en 200 mg de pFSH en ocho dosis decrecientes (intervalos de 12 h) y estrategias de apoyo tales como el cambio del CIDR al día 7, el rociado de los cuernos durante el procedimiento con solución salina con heparina y el uso de Flunixin Meglumine. No se encontraron diferencias significativas en el número total de estructuras recuperadas durante los años de aplicación del protocolo de superovulación (G1=8±2, G2=18±3, G3=19±4; p>0.05). Se observó una alta repetibilidad, tanto para el número total de embriones como para el número de embriones transferibles en G2 y G3. El tratamiento superovulatorio durante 3 y 4 años sucesivos no afectó la eficacia de la recuperación de embriones. Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2023-12-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article application/pdf https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/24604 10.15381/rivep.v34i6.24604 Revista de Investigaciones Veterinarias del Perú; Vol. 34 No. 6 (2023); e24604 Revista de Investigaciones Veterinarias del Perú; Vol. 34 Núm. 6 (2023); e24604 1682-3419 1609-9117 spa https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/24604/20383 Derechos de autor 2023 Marcela Gómez-Ceruti, Carlos Mariano Serrano, Giordano Catenacci A., Lina Maria Correa E., Claudia Letelier-Velasquez http://creativecommons.org/licenses/by/4.0 |
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Gómez-Ceruti, Marcela Serrano, Carlos Mariano Catenacci A., Giordano Correa E., Lina Maria Letelier-Velasquez, Claudia |
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Gómez-Ceruti, Marcela Serrano, Carlos Mariano Catenacci A., Giordano Correa E., Lina Maria Letelier-Velasquez, Claudia Repeatability of the superovulatory response in embryo production in vivo with a modified pFSH protocol in Australian Merino sheep |
author_facet |
Gómez-Ceruti, Marcela Serrano, Carlos Mariano Catenacci A., Giordano Correa E., Lina Maria Letelier-Velasquez, Claudia |
author_sort |
Gómez-Ceruti, Marcela |
title |
Repeatability of the superovulatory response in embryo production in vivo with a modified pFSH protocol in Australian Merino sheep |
title_short |
Repeatability of the superovulatory response in embryo production in vivo with a modified pFSH protocol in Australian Merino sheep |
title_full |
Repeatability of the superovulatory response in embryo production in vivo with a modified pFSH protocol in Australian Merino sheep |
title_fullStr |
Repeatability of the superovulatory response in embryo production in vivo with a modified pFSH protocol in Australian Merino sheep |
title_full_unstemmed |
Repeatability of the superovulatory response in embryo production in vivo with a modified pFSH protocol in Australian Merino sheep |
title_sort |
repeatability of the superovulatory response in embryo production in vivo with a modified pfsh protocol in australian merino sheep |
description |
The study evaluated the repeatability of the superovulatory response in 12 Australian Merino ewes during the breeding season in one (G1), three (G2), and four (G3) consecutive years. A modified protocol was used based on 200 mg pFSH in eight decreasing doses (12-h intervals) and supportive strategies such as changing the CIDR at day 7, spraying the horns with heparinized saline during the procedure, and use of Flunixin Meglumine. No significant differences were found in the total number of structures recovered during the years of application of the superovulation protocol (G1=8±2, G2=18±3, G3=19±4; p>0.05). High repeatability was observed, both for the total number of embryos and for the number of transferable embryos in G2 and G3. Superovulatory treatment for 3 and 4 successive years did not affect the efficacy of embryo retrieval. |
publisher |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
publishDate |
2023 |
url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/24604 |
work_keys_str_mv |
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