Molecular evaluation of the presence of Perkinsus spp in samples of scallops (Argopecten purpuratus) from northern Peru

Molecular evaluation of the presence of Perkinsus spp in samples of scallops (Argopecten purpuratus) from northern Peru

The aim of this study was to evaluate the presence of Perkinsus spp, a parasite that is mandatory to be report to the OIE, in samples of calico scallop (Argopecten purpuratus) from Ancash, Peru, using the PCR technique. In total, 56 samples were selected from those collected for a previous study in 2015. The selected samples were gill tissues with histological lesions suggestive of infection with the protozoan (hemocytic infiltration and necrosis). DNA was extracted from the samples and specific primers were used to detect Perkinsus spp by PCR. In addition, the detection limit of the technique was evaluated. All samples were negative. It was determined that 1.63 ng was the least detectable template DNA in one of the positive controls.

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Main Authors: Vivanco S., Manuela, Valera A., Adhemir, Llanco A., Luis, Serrano-Martínez, Enrique
Format: Digital revista
Language:spa
Published: Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2020
Online Access:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/19044
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spelling oai:ojs.csi.unmsm:article-190442020-12-21T22:00:34Z Molecular evaluation of the presence of Perkinsus spp in samples of scallops (Argopecten purpuratus) from northern Peru Evaluación molecular de la presencia de Perkinsus spp en muestras de concha de abanico (Argopecten purpuratus) del norte del Perú Vivanco S., Manuela Valera A., Adhemir Llanco A., Luis Serrano-Martínez, Enrique Perkinsus Argopecten purpuratus PCR Perkinsus Argopecten purpuratus PCR The aim of this study was to evaluate the presence of Perkinsus spp, a parasite that is mandatory to be report to the OIE, in samples of calico scallop (Argopecten purpuratus) from Ancash, Peru, using the PCR technique. In total, 56 samples were selected from those collected for a previous study in 2015. The selected samples were gill tissues with histological lesions suggestive of infection with the protozoan (hemocytic infiltration and necrosis). DNA was extracted from the samples and specific primers were used to detect Perkinsus spp by PCR. In addition, the detection limit of the technique was evaluated. All samples were negative. It was determined that 1.63 ng was the least detectable template DNA in one of the positive controls. El objetivo de este estudio fue evaluar la presencia de Perkinsus spp, parásito de reporte obligatorio ante la OIE, en muestras de concha de abanico (Argopecten purpuratus) de Ancash, Perú, mediante la técnica de PCR. Se seleccionaron 56 muestras de las colectadas para un estudio previo en 2015. Las muestras seleccionadas fueron tejidos de branquia con lesiones histológicas sugestivas de infección con el protozoo (infiltración hemocítica y necrosis). Se extrajo el ADN de las muestras y se utilizaron cebadores específicos para detectar Perkinsus spp por PCR. Además, se evaluó el límite de detección de la técnica. Todas las muestras resultaron negativas. Se determinó que 1.63 ng fue la menor cantidad de DNA molde detectable en uno de los controles positivos. Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2020-12-21 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article application/pdf https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/19044 10.15381/rivep.v31i4.19044 Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 4 (2020); e19044 Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 4 (2020); e19044 1682-3419 1609-9117 spa https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/19044/16141 Derechos de autor 2020 Manuela Vivanco S., Adhemir Valera A., Luis Llanco A., Enrique Serrano-Martínez https://creativecommons.org/licenses/by-nc-sa/4.0
institution UNMSM
collection OJS
country Perú
countrycode PE
component Revista
access En linea
databasecode rev-rivep
tag revista
region America del Sur
libraryname Biblioteca de la Facultad de Medicina Veterinaria
language spa
format Digital
author Vivanco S., Manuela
Valera A., Adhemir
Llanco A., Luis
Serrano-Martínez, Enrique
spellingShingle Vivanco S., Manuela
Valera A., Adhemir
Llanco A., Luis
Serrano-Martínez, Enrique
Molecular evaluation of the presence of Perkinsus spp in samples of scallops (Argopecten purpuratus) from northern Peru
author_facet Vivanco S., Manuela
Valera A., Adhemir
Llanco A., Luis
Serrano-Martínez, Enrique
author_sort Vivanco S., Manuela
title Molecular evaluation of the presence of Perkinsus spp in samples of scallops (Argopecten purpuratus) from northern Peru
title_short Molecular evaluation of the presence of Perkinsus spp in samples of scallops (Argopecten purpuratus) from northern Peru
title_full Molecular evaluation of the presence of Perkinsus spp in samples of scallops (Argopecten purpuratus) from northern Peru
title_fullStr Molecular evaluation of the presence of Perkinsus spp in samples of scallops (Argopecten purpuratus) from northern Peru
title_full_unstemmed Molecular evaluation of the presence of Perkinsus spp in samples of scallops (Argopecten purpuratus) from northern Peru
title_sort molecular evaluation of the presence of perkinsus spp in samples of scallops (argopecten purpuratus) from northern peru
description The aim of this study was to evaluate the presence of Perkinsus spp, a parasite that is mandatory to be report to the OIE, in samples of calico scallop (Argopecten purpuratus) from Ancash, Peru, using the PCR technique. In total, 56 samples were selected from those collected for a previous study in 2015. The selected samples were gill tissues with histological lesions suggestive of infection with the protozoan (hemocytic infiltration and necrosis). DNA was extracted from the samples and specific primers were used to detect Perkinsus spp by PCR. In addition, the detection limit of the technique was evaluated. All samples were negative. It was determined that 1.63 ng was the least detectable template DNA in one of the positive controls.
publisher Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publishDate 2020
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/19044
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