Detection of Salmonella Javiana in taricaya turtles (Podocnemis unifilis) in two zoos in Peru

Detection of Salmonella Javiana in taricaya turtles (Podocnemis unifilis) in two zoos in Peru

The aim of this study was to serotype and determine the antibacterial sensitivity profile of Salmonella spp strains isolated from taricaya turtles (Podocnemis unifilis) from the Huachipa Zoological Park (n = 30). Lima, and the Natural Park of Pucallpa (n = 43), Ucayali, located in the coastal and jungle region of Peru, respectively. A total of 73 samples of cloacal swabs were obtained that were inoculated in Stuart bacterial transport media, enrichment media (tetrathionate broth) and selective media (XLD agar and Salmonella-Shigella), finding typical lactose-negative colonies of Salmonella, which were verified by biochemical tests standard (4.11%, 3/73). The results of the serological typing using the Kauffmann-White scheme showed that two of the three strains corresponded to Salmonella Javiana (O9,12: I, Z28: H5) and the other as not determined. The three positive samples were from juvenile turtles, of indefinite sex and from one of the two centers under study. Antimicrobial sensitivity tests determined that the strains of S. Javiana were sensitive to streptomycin, trimetopim sulfamethoxazole, fosfomycin, furazolidone, gentamicin and chloramphenicol and resistant to enrofloxacin, and tetracycline.

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Main Authors: Campos N., Andrea, Morales-Cauti, Siever, Navarro O., Armando, Eslava C., Carlos
Format: Digital revista
Language:spa
Published: Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2020
Online Access:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17554
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spelling oai:ojs.csi.unmsm:article-175542020-03-30T05:45:29Z Detection of Salmonella Javiana in taricaya turtles (Podocnemis unifilis) in two zoos in Peru Detección de Salmonella Javiana en tortugas taricaya (Podocnemis unifilis) en dos parques zoológicos del Perú Campos N., Andrea Morales-Cauti, Siever Navarro O., Armando Eslava C., Carlos taricaya cloacal Salmonella spp S. javiana serotyping taricaya cloaca Salmonella spp Salmonella Javiana serotipificación The aim of this study was to serotype and determine the antibacterial sensitivity profile of Salmonella spp strains isolated from taricaya turtles (Podocnemis unifilis) from the Huachipa Zoological Park (n = 30). Lima, and the Natural Park of Pucallpa (n = 43), Ucayali, located in the coastal and jungle region of Peru, respectively. A total of 73 samples of cloacal swabs were obtained that were inoculated in Stuart bacterial transport media, enrichment media (tetrathionate broth) and selective media (XLD agar and Salmonella-Shigella), finding typical lactose-negative colonies of Salmonella, which were verified by biochemical tests standard (4.11%, 3/73). The results of the serological typing using the Kauffmann-White scheme showed that two of the three strains corresponded to Salmonella Javiana (O9,12: I, Z28: H5) and the other as not determined. The three positive samples were from juvenile turtles, of indefinite sex and from one of the two centers under study. Antimicrobial sensitivity tests determined that the strains of S. Javiana were sensitive to streptomycin, trimetopim sulfamethoxazole, fosfomycin, furazolidone, gentamicin and chloramphenicol and resistant to enrofloxacin, and tetracycline. El presente estudio tuvo por objetivo serotipificar y determinar el perfil de sensibilidad a antibacterianos de cepas de Salmonella spp aisladas de tortugas taricaya (Podocnemis unifilis) del Parque Zoológico Huachipa (n = 30), Lima, y del Parque Natural de Pucallpa (n = 43), Ucayali, ubicados en la región costa y selva del Perú, respectivamente. Se obtuvieron 73 muestras de hisopados cloacales que fueron inoculados en medios de transporte bacteriano Stuart, medios de enriquecimiento (caldo de tetrationato) y medios selectivos (agar XLD y Salmonella-Shigella), encontrando colonias lactosa negativas típicas de Salmonella, que fueron comprobadas mediante pruebas bioquímicas estándar (4.11%, 3/73). Los resultados de la tipificación serológica utilizando el esquema de Kauffmann-White mostraron que dos de las tres cepas correspondieron a Salmonella Javiana (O9,12:I,Z28:H5) y la otra como no determinada. Las tres muestras positivas fueron de tortugas juveniles, de sexo indefinido y de uno de los dos centros en estudio. Las pruebas de sensibilidad frente a antimicrobianos determinaron que las cepas de S. Javiana fueron sensibles a estreptomicina, trimetopim sulfametoxazol, fosfomicina, furazolidona, gentamicina y cloranfenicol y resistentes a enrofloxacina, y tetraciclina. Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2020-03-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article application/pdf https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17554 10.15381/rivep.v31i1.17554 Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 1 (2020); e17554 Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 1 (2020); e17554 1682-3419 1609-9117 spa https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17554/14822 Derechos de autor 2020 Andrea Campos N., Siever Morales-Cauti, Armando Navarro O., Carlos Eslava C. https://creativecommons.org/licenses/by-nc-sa/4.0
institution UNMSM
collection OJS
country Perú
countrycode PE
component Revista
access En linea
databasecode rev-rivep
tag revista
region America del Sur
libraryname Biblioteca de la Facultad de Medicina Veterinaria
language spa
format Digital
author Campos N., Andrea
Morales-Cauti, Siever
Navarro O., Armando
Eslava C., Carlos
spellingShingle Campos N., Andrea
Morales-Cauti, Siever
Navarro O., Armando
Eslava C., Carlos
Detection of Salmonella Javiana in taricaya turtles (Podocnemis unifilis) in two zoos in Peru
author_facet Campos N., Andrea
Morales-Cauti, Siever
Navarro O., Armando
Eslava C., Carlos
author_sort Campos N., Andrea
title Detection of Salmonella Javiana in taricaya turtles (Podocnemis unifilis) in two zoos in Peru
title_short Detection of Salmonella Javiana in taricaya turtles (Podocnemis unifilis) in two zoos in Peru
title_full Detection of Salmonella Javiana in taricaya turtles (Podocnemis unifilis) in two zoos in Peru
title_fullStr Detection of Salmonella Javiana in taricaya turtles (Podocnemis unifilis) in two zoos in Peru
title_full_unstemmed Detection of Salmonella Javiana in taricaya turtles (Podocnemis unifilis) in two zoos in Peru
title_sort detection of salmonella javiana in taricaya turtles (podocnemis unifilis) in two zoos in peru
description The aim of this study was to serotype and determine the antibacterial sensitivity profile of Salmonella spp strains isolated from taricaya turtles (Podocnemis unifilis) from the Huachipa Zoological Park (n = 30). Lima, and the Natural Park of Pucallpa (n = 43), Ucayali, located in the coastal and jungle region of Peru, respectively. A total of 73 samples of cloacal swabs were obtained that were inoculated in Stuart bacterial transport media, enrichment media (tetrathionate broth) and selective media (XLD agar and Salmonella-Shigella), finding typical lactose-negative colonies of Salmonella, which were verified by biochemical tests standard (4.11%, 3/73). The results of the serological typing using the Kauffmann-White scheme showed that two of the three strains corresponded to Salmonella Javiana (O9,12: I, Z28: H5) and the other as not determined. The three positive samples were from juvenile turtles, of indefinite sex and from one of the two centers under study. Antimicrobial sensitivity tests determined that the strains of S. Javiana were sensitive to streptomycin, trimetopim sulfamethoxazole, fosfomycin, furazolidone, gentamicin and chloramphenicol and resistant to enrofloxacin, and tetracycline.
publisher Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publishDate 2020
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17554
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