Evaluation of dimethylacetamide and dimethylformamide as cryoprotectants for epididymal alpaca sperm (Vicugna pacos)
The aim of this study was to determine the effect of the type (dimethylacetamide [DMA] and dimethylformamide [DMF]) and concentrations (1, 3.5, 7%) of cryoprotective agents on three sperm parameters during the cryopreservation process of epididymal alpaca sperm. A 2x3 factorial design consisting of the two cryoprotectants and the three concentrations was used. The parameters of sperm viability (SYBR-14/PI) and mitochondrial membrane potential (MitoTracker Deep Red FM) of sperm of 40 epididymis were evaluated by flow cytometry and motility by microscopy after thawing. 111/5000. A higher percentage of motility after thawing was found with AMD (14%) than with DMF (10%) (p=0.001). In addition, there was a significant effect of the cryoprotectant concentration in all the parameters evaluated (p<0.001), being better at concentrations of 1 and 3.5% than with 7%. No significant effect of the cryoprotectant-concentration interaction was found.
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Digital revista |
| Language: | spa |
| Published: |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
2020
|
| Online Access: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17548 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
oai:ojs.csi.unmsm:article-17548 |
|---|---|
| record_format |
ojs |
| spelling |
oai:ojs.csi.unmsm:article-175482020-03-30T05:45:29Z Evaluation of dimethylacetamide and dimethylformamide as cryoprotectants for epididymal alpaca sperm (Vicugna pacos) Evaluación de dimetilacetamida y dimetilformamida como agentes crioprotectores para espermatozoides epididimarios de alpaca (Vicugna pacos) Contreras, Winnie Ruiz García, Luis Santiani, Alexei Vicugna pacos cryopreservation spermatozoa epididymis dimethylacetamide dimethylformamide cytometry Vicugna pacos criopreservación espermatozoides epidídimo dimetilacetamida dimetilformamida citometría The aim of this study was to determine the effect of the type (dimethylacetamide [DMA] and dimethylformamide [DMF]) and concentrations (1, 3.5, 7%) of cryoprotective agents on three sperm parameters during the cryopreservation process of epididymal alpaca sperm. A 2x3 factorial design consisting of the two cryoprotectants and the three concentrations was used. The parameters of sperm viability (SYBR-14/PI) and mitochondrial membrane potential (MitoTracker Deep Red FM) of sperm of 40 epididymis were evaluated by flow cytometry and motility by microscopy after thawing. 111/5000. A higher percentage of motility after thawing was found with AMD (14%) than with DMF (10%) (p=0.001). In addition, there was a significant effect of the cryoprotectant concentration in all the parameters evaluated (p<0.001), being better at concentrations of 1 and 3.5% than with 7%. No significant effect of the cryoprotectant-concentration interaction was found. El objetivo del presente estudio fue determinar el efecto de dos tipos (dimetilacetamida [DMA] y dimetilformamida [DMF]) y tres concentraciones (1, 3.5, 7%) de agentes crioprotectores sobre parámetros espermáticos durante el proceso de criopreservación de espermatozoides epididimarios de alpaca. Se empleó un diseño factorial 2 x 3 formado por los dos crioprotectores y las tres concentraciones. Se evaluaron los parámetros de viabilidad espermática (SYBR-14/PI) y potencial de membrana mitocondrial (MitoTracker Deep Red FM) mediante citometría de flujo y motilidad pos-descongelación por microscopía de muestras de material espermático de 40 epidídimos. Se encontró un mayor porcentaje de motilidad pos-descongelamiento con DMA (14%) que con DMF (10%) (p=0.001). Además, hubo efecto significativo de la concentración del crioprotector en todos los parámetros evaluados (p<0.001), siendo mejor en las concentraciones de 1 y 3.5% que con 7%. No se halló efecto significativo de la interacción crioprotector-concentración. Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2020-03-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article application/pdf https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17548 10.15381/rivep.v31i1.17548 Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 1 (2020); e17548 Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 1 (2020); e17548 1682-3419 1609-9117 spa https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17548/14813 Derechos de autor 2020 Winnie Contreras, Luis Ruiz García, Alexei Santiani https://creativecommons.org/licenses/by-nc-sa/4.0 |
| institution |
UNMSM |
| collection |
OJS |
| country |
Perú |
| countrycode |
PE |
| component |
Revista |
| access |
En linea |
| databasecode |
rev-rivep |
| tag |
revista |
| region |
America del Sur |
| libraryname |
Biblioteca de la Facultad de Medicina Veterinaria |
| language |
spa |
| format |
Digital |
| author |
Contreras, Winnie Ruiz García, Luis Santiani, Alexei |
| spellingShingle |
Contreras, Winnie Ruiz García, Luis Santiani, Alexei Evaluation of dimethylacetamide and dimethylformamide as cryoprotectants for epididymal alpaca sperm (Vicugna pacos) |
| author_facet |
Contreras, Winnie Ruiz García, Luis Santiani, Alexei |
| author_sort |
Contreras, Winnie |
| title |
Evaluation of dimethylacetamide and dimethylformamide as cryoprotectants for epididymal alpaca sperm (Vicugna pacos) |
| title_short |
Evaluation of dimethylacetamide and dimethylformamide as cryoprotectants for epididymal alpaca sperm (Vicugna pacos) |
| title_full |
Evaluation of dimethylacetamide and dimethylformamide as cryoprotectants for epididymal alpaca sperm (Vicugna pacos) |
| title_fullStr |
Evaluation of dimethylacetamide and dimethylformamide as cryoprotectants for epididymal alpaca sperm (Vicugna pacos) |
| title_full_unstemmed |
Evaluation of dimethylacetamide and dimethylformamide as cryoprotectants for epididymal alpaca sperm (Vicugna pacos) |
| title_sort |
evaluation of dimethylacetamide and dimethylformamide as cryoprotectants for epididymal alpaca sperm (vicugna pacos) |
| description |
The aim of this study was to determine the effect of the type (dimethylacetamide [DMA] and dimethylformamide [DMF]) and concentrations (1, 3.5, 7%) of cryoprotective agents on three sperm parameters during the cryopreservation process of epididymal alpaca sperm. A 2x3 factorial design consisting of the two cryoprotectants and the three concentrations was used. The parameters of sperm viability (SYBR-14/PI) and mitochondrial membrane potential (MitoTracker Deep Red FM) of sperm of 40 epididymis were evaluated by flow cytometry and motility by microscopy after thawing. 111/5000. A higher percentage of motility after thawing was found with AMD (14%) than with DMF (10%) (p=0.001). In addition, there was a significant effect of the cryoprotectant concentration in all the parameters evaluated (p<0.001), being better at concentrations of 1 and 3.5% than with 7%. No significant effect of the cryoprotectant-concentration interaction was found. |
| publisher |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| publishDate |
2020 |
| url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17548 |
| work_keys_str_mv |
AT contreraswinnie evaluationofdimethylacetamideanddimethylformamideascryoprotectantsforepididymalalpacaspermvicugnapacos AT ruizgarcialuis evaluationofdimethylacetamideanddimethylformamideascryoprotectantsforepididymalalpacaspermvicugnapacos AT santianialexei evaluationofdimethylacetamideanddimethylformamideascryoprotectantsforepididymalalpacaspermvicugnapacos AT contreraswinnie evaluaciondedimetilacetamidaydimetilformamidacomoagentescrioprotectoresparaespermatozoidesepididimariosdealpacavicugnapacos AT ruizgarcialuis evaluaciondedimetilacetamidaydimetilformamidacomoagentescrioprotectoresparaespermatozoidesepididimariosdealpacavicugnapacos AT santianialexei evaluaciondedimetilacetamidaydimetilformamidacomoagentescrioprotectoresparaespermatozoidesepididimariosdealpacavicugnapacos |
| _version_ |
1763178954168991744 |