Genetic characterization and antimicrobial resistance patterns of Salmonella enterica subsp. enterica serovar Typhimurium in guinea pigs under intensive breeding

Genetic characterization and antimicrobial resistance patterns of Salmonella enterica subsp. enterica serovar Typhimurium in guinea pigs under intensive breeding

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The aim of this study was to conduct a phenotypic and genetic characterization of 35 Salmonella Typhimurium isolates from guinea pig production systems in the Lima, Peru in relation to antimicrobial resistance. The profile of 11 antibiotics (florfenicol, sulfamethoxazole, doxycycline, oxytetracycline, amoxicillin, enrofloxacin, norfloxacin, levofloxacin, ciprofloxacin, colistin and fosfomycin), genotyping by ERIC-PCR techniques and quinolone resistance gene profile (qnrB, qnRD, qnRD, qnrD, qnrD , aa6), tetracyclines (tetA, tetB, tetC, tetD), phenols (cat1, cat2, cmlA, cmlB) and sulfamethoxazole (sul1, sul2). The results indicate the presence of multidrug resistance in 80% (n=28) of the strains, being the most common resistance to the antibiotic colistin (91.4%), followed by sulfamethoxazole (68.6%) and enrofloxacin (62.9%), and with a moderate resistance to amoxicillin (20%), ciprofloxacin (20%) and norfloxacin (11.43%). Nine of 14 resistance genes were detected, with a higher frequency of the tetB genes (71.4%), sulI (57.1%) and cat2 (48.6%). The presence of seven differentiated genetic profiles (A-G) distributed in two genetic clusters was observed, the most frequent was the D profile (54.3%) followed by the C profile (28.6%). The results suggest the presence of Salmonella Typhimurium strains with moderate variability and multidrug resistance profiles with the presence of resistance genes, mainly for tetracyclines and sulfonamides.

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Main Authors: Huamán, Meylin, Pérez, Crhistian, Rodríguez, Jorge, Killerby, Marjorie, Lovón, Stephane, Chauca, Lilia
Format: Digital revista
Language:spa
Published: Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2020
Online Access:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17542
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id oai:ojs.csi.unmsm:article-17542
record_format ojs
institution UNMSM
collection OJS
country Perú
countrycode PE
component Revista
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databasecode rev-rivep
tag revista
region America del Sur
libraryname Biblioteca de la Facultad de Medicina Veterinaria
language spa
format Digital
author Huamán, Meylin
Pérez, Crhistian
Rodríguez, Jorge
Killerby, Marjorie
Lovón, Stephane
Chauca, Lilia
spellingShingle Huamán, Meylin
Pérez, Crhistian
Rodríguez, Jorge
Killerby, Marjorie
Lovón, Stephane
Chauca, Lilia
Genetic characterization and antimicrobial resistance patterns of Salmonella enterica subsp. enterica serovar Typhimurium in guinea pigs under intensive breeding
author_facet Huamán, Meylin
Pérez, Crhistian
Rodríguez, Jorge
Killerby, Marjorie
Lovón, Stephane
Chauca, Lilia
author_sort Huamán, Meylin
title Genetic characterization and antimicrobial resistance patterns of Salmonella enterica subsp. enterica serovar Typhimurium in guinea pigs under intensive breeding
title_short Genetic characterization and antimicrobial resistance patterns of Salmonella enterica subsp. enterica serovar Typhimurium in guinea pigs under intensive breeding
title_full Genetic characterization and antimicrobial resistance patterns of Salmonella enterica subsp. enterica serovar Typhimurium in guinea pigs under intensive breeding
title_fullStr Genetic characterization and antimicrobial resistance patterns of Salmonella enterica subsp. enterica serovar Typhimurium in guinea pigs under intensive breeding
title_full_unstemmed Genetic characterization and antimicrobial resistance patterns of Salmonella enterica subsp. enterica serovar Typhimurium in guinea pigs under intensive breeding
title_sort genetic characterization and antimicrobial resistance patterns of salmonella enterica subsp. enterica serovar typhimurium in guinea pigs under intensive breeding
description The aim of this study was to conduct a phenotypic and genetic characterization of 35 Salmonella Typhimurium isolates from guinea pig production systems in the Lima, Peru in relation to antimicrobial resistance. The profile of 11 antibiotics (florfenicol, sulfamethoxazole, doxycycline, oxytetracycline, amoxicillin, enrofloxacin, norfloxacin, levofloxacin, ciprofloxacin, colistin and fosfomycin), genotyping by ERIC-PCR techniques and quinolone resistance gene profile (qnrB, qnRD, qnRD, qnrD, qnrD , aa6), tetracyclines (tetA, tetB, tetC, tetD), phenols (cat1, cat2, cmlA, cmlB) and sulfamethoxazole (sul1, sul2). The results indicate the presence of multidrug resistance in 80% (n=28) of the strains, being the most common resistance to the antibiotic colistin (91.4%), followed by sulfamethoxazole (68.6%) and enrofloxacin (62.9%), and with a moderate resistance to amoxicillin (20%), ciprofloxacin (20%) and norfloxacin (11.43%). Nine of 14 resistance genes were detected, with a higher frequency of the tetB genes (71.4%), sulI (57.1%) and cat2 (48.6%). The presence of seven differentiated genetic profiles (A-G) distributed in two genetic clusters was observed, the most frequent was the D profile (54.3%) followed by the C profile (28.6%). The results suggest the presence of Salmonella Typhimurium strains with moderate variability and multidrug resistance profiles with the presence of resistance genes, mainly for tetracyclines and sulfonamides.
publisher Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publishDate 2020
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17542
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spelling oai:ojs.csi.unmsm:article-175422020-03-30T05:45:29Z Genetic characterization and antimicrobial resistance patterns of Salmonella enterica subsp. enterica serovar Typhimurium in guinea pigs under intensive breeding Caracterización genética y patrones de resistencia antimicrobiana en cepas de Salmonella enterica subsp. enterica serovar Typhimurium en cuyes de crianza intensiva Huamán, Meylin Pérez, Crhistian Rodríguez, Jorge Killerby, Marjorie Lovón, Stephane Chauca, Lilia Salmonella typhimurium antibiotic resistance genotyping ERIC-PCR Salmonella typhimurium resistencia a antibióticos genotipificación ERIC-PCR The aim of this study was to conduct a phenotypic and genetic characterization of 35 Salmonella Typhimurium isolates from guinea pig production systems in the Lima, Peru in relation to antimicrobial resistance. The profile of 11 antibiotics (florfenicol, sulfamethoxazole, doxycycline, oxytetracycline, amoxicillin, enrofloxacin, norfloxacin, levofloxacin, ciprofloxacin, colistin and fosfomycin), genotyping by ERIC-PCR techniques and quinolone resistance gene profile (qnrB, qnRD, qnRD, qnrD, qnrD , aa6), tetracyclines (tetA, tetB, tetC, tetD), phenols (cat1, cat2, cmlA, cmlB) and sulfamethoxazole (sul1, sul2). The results indicate the presence of multidrug resistance in 80% (n=28) of the strains, being the most common resistance to the antibiotic colistin (91.4%), followed by sulfamethoxazole (68.6%) and enrofloxacin (62.9%), and with a moderate resistance to amoxicillin (20%), ciprofloxacin (20%) and norfloxacin (11.43%). Nine of 14 resistance genes were detected, with a higher frequency of the tetB genes (71.4%), sulI (57.1%) and cat2 (48.6%). The presence of seven differentiated genetic profiles (A-G) distributed in two genetic clusters was observed, the most frequent was the D profile (54.3%) followed by the C profile (28.6%). The results suggest the presence of Salmonella Typhimurium strains with moderate variability and multidrug resistance profiles with the presence of resistance genes, mainly for tetracyclines and sulfonamides. El objetivo del estudio fue realizar la caracterización fenotípica y genética de 35 aislados de Salmonella Typhimurium provenientes de sistemas de producción de cuyes en la región Lima, Perú, con relación a la resistencia a antimicrobianos. Se determinó el perfil de 11 antibióticos (florfenicol, sulfametoxazol, doxiciclina, oxitetraciclina, amoxicilina, enrofloxacina, norfloxacina, levofloxacina, ciprofloxacina, colistina y fosfomicina), genotipificación por técnicas de ERIC-PCR y perfil de genes de resistencia a quinolonas (qnrB, qnrD, qnrR, aa6), tetraciclinas (tetA, tetB, tetC, tetD), fenicoles (cat1, cat2, cmlA, cmlB) y sulfametoxazol (sul1, sul2). Los resultados indican la presencia de multidrogo resistencia en un 80% (n=28) de las cepas, siendo la resistencia más común al antibiótico colistina (91.4%), seguido del sulfametoxazol (68.6%) y enrofloxacina (62.9%), y con una moderada resistencia a amoxicilina (20%), ciprofloxacina (20%) y norfloxacina (11.43%). Nueve de 14 genes de resistencia fueron detectados, con una mayor frecuencia de los genes tetB (71.4%), sulI (57.1%) y cat2 (48.6%). Se observó la presencia de siete perfiles genéticos diferenciados (A-G) distribuidos en dos clústeres genéticos, siendo el perfil D más frecuente (54.3%) seguido del perfil C (28.6%) de frecuencia. Los resultados sugieren la presencia de cepas de Salmonella Typhimurium con moderada variabilidad y perfiles de multidrogo resistencia con la presencia de genes de resistencia, principalmente para tetraciclinas y sulfonamidas. Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2020-03-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article application/pdf https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17542 10.15381/rivep.v31i1.17542 Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 1 (2020); e17542 Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 1 (2020); e17542 1682-3419 1609-9117 spa https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17542/14818 Derechos de autor 2020 Meylin Huamán, Crhistian Pérez, Jorge Rodríguez, Marjorie Killerby, Stephane Lovón, Lilia Chauca https://creativecommons.org/licenses/by-nc-sa/4.0

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