Real time PCR for detection of Hepatozoon canis in dogs from the city of Cúcuta, Colombia

Real time PCR for detection of Hepatozoon canis in dogs from the city of Cúcuta, Colombia

Hepatozoon canis are protozoa transmitted to dogs mainly by ticks of the Rhipicephalus sanguineus species. In Colombia, H. canis has been detected; however, only one study using the PCR technique is available detecting this hemoparasite. This study reports a frequency of infection of 8.6% (30/350 samples). H. canis was detected in blood samples from domestic dogs in the city of Cucuta (Colombia), by real-time PCR using hybridization probes with duplex detection and internal control of GADPH amplification, using primers and probes designed in this study.

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Main Authors: Chinchilla, Danny, Thomas, Richard, Castro, Lyda Raquel
Format: Digital revista
Language:spa
Published: Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2020
Online Access:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/16429
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spelling oai:ojs.csi.unmsm:article-164292020-09-29T21:58:01Z Real time PCR for detection of Hepatozoon canis in dogs from the city of Cúcuta, Colombia PCR tiempo real para detección de Hepatozoon canis en perros de la ciudad de Cúcuta, Colombia Chinchilla, Danny Thomas, Richard Castro, Lyda Raquel Apicomplexa canines molecular detection hemoparasites Apicomplexa caninos detección molecular hemoparásito Hepatozoon canis are protozoa transmitted to dogs mainly by ticks of the Rhipicephalus sanguineus species. In Colombia, H. canis has been detected; however, only one study using the PCR technique is available detecting this hemoparasite. This study reports a frequency of infection of 8.6% (30/350 samples). H. canis was detected in blood samples from domestic dogs in the city of Cucuta (Colombia), by real-time PCR using hybridization probes with duplex detection and internal control of GADPH amplification, using primers and probes designed in this study. Hepatozoon canis son protozoarios transmitidos a perros principalmente por garrapatas de la especie Rhipicephalus sanguineus. En Colombia se ha detectado H. canis; sin embargo, solo se dispone de un estudio empleando la técnica de PCR para la detección de este hemoparásito. En este trabajo se reporta una frecuencia de infección de 8.6% (30/350 muestras). Se detectó la presencia de de H. canis en muestras de sangre de caninos domésticos de la ciudad de Cúcuta (Colombia), mediante PCR en tiempo real, empleando la tecnología de sondas de hibridación con detección dúplex y control interno de amplificación GADPH, utilizando cebadores y sondas diseñados en el presente estudio.   Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2020-09-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article application/pdf https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/16429 10.15381/rivep.v31i3.16429 Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 3 (2020); e16429 Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 3 (2020); e16429 1682-3419 1609-9117 spa https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/16429/15835 Derechos de autor 2020 Danny Chinchilla, Richard Thomas, Lyda Raquel Castro https://creativecommons.org/licenses/by-nc-sa/4.0
institution UNMSM
collection OJS
country Perú
countrycode PE
component Revista
access En linea
databasecode rev-rivep
tag revista
region America del Sur
libraryname Biblioteca de la Facultad de Medicina Veterinaria
language spa
format Digital
author Chinchilla, Danny
Thomas, Richard
Castro, Lyda Raquel
spellingShingle Chinchilla, Danny
Thomas, Richard
Castro, Lyda Raquel
Real time PCR for detection of Hepatozoon canis in dogs from the city of Cúcuta, Colombia
author_facet Chinchilla, Danny
Thomas, Richard
Castro, Lyda Raquel
author_sort Chinchilla, Danny
title Real time PCR for detection of Hepatozoon canis in dogs from the city of Cúcuta, Colombia
title_short Real time PCR for detection of Hepatozoon canis in dogs from the city of Cúcuta, Colombia
title_full Real time PCR for detection of Hepatozoon canis in dogs from the city of Cúcuta, Colombia
title_fullStr Real time PCR for detection of Hepatozoon canis in dogs from the city of Cúcuta, Colombia
title_full_unstemmed Real time PCR for detection of Hepatozoon canis in dogs from the city of Cúcuta, Colombia
title_sort real time pcr for detection of hepatozoon canis in dogs from the city of cúcuta, colombia
description Hepatozoon canis are protozoa transmitted to dogs mainly by ticks of the Rhipicephalus sanguineus species. In Colombia, H. canis has been detected; however, only one study using the PCR technique is available detecting this hemoparasite. This study reports a frequency of infection of 8.6% (30/350 samples). H. canis was detected in blood samples from domestic dogs in the city of Cucuta (Colombia), by real-time PCR using hybridization probes with duplex detection and internal control of GADPH amplification, using primers and probes designed in this study.
publisher Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publishDate 2020
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/16429
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