Evaluation of two cryprotectans and three curves of programable freezing in semen cryopreservation of Brycon henni (Pisces: Characidae)

Evaluation of two cryprotectans and three curves of programable freezing in semen cryopreservation of Brycon henni (Pisces: Characidae)

The aim of this study was to evaluate the cryopreservation of Brycon henni semen using three programmable freezing curves and two permeable cryoprotectants. Semen of 80 males were diluted in medium supplemented with ethylene glycol (EG) or dimethylsulfoxide (DMSO), and cryopreserved in semen straws using three programmable freezing curves: slow (66 min, -0.42 °C/min), medium (43.3 min, -0.6 °C/min) and ultra-rapid (7.7 min, -5.19 °C/min). After one month of storage, semen was thawed and motility was evaluated through a class analysis system (SCA®) and sperm vitality (EV) by fluorescence microscopy with SYBR14/IP probes. For statistical analysis, generalized linear models (GLM) were adjusted and means compared by the Tukey test. The medium and ultrarapid velocity curves showed higher and equivalent values for total motility, progressive motility, and linear velocity of sperm (p<0.05). Sperm vitality was better in the medium velocity curve (52.4 ± 8.6%) in comparison with the ultra-rapid (43.0 ± 19.4%) and slow (29.0 ± 11.8%) curves (p<0.05). EG showed greater sperm vitality (p<0.05). It is concluded that freezing of sabaleta semen (B. henni) by a medium velocity programmable freezing curve and ethylene glycol as cryoprotectant allows better results of post-thaw semen quality.

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Main Authors: Restrepo-Betancur, Giovanni, Montoya Páez, Juan David, Arboleda Chacón, Lucy
Format: Digital revista
Language:spa
Published: Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2017
Online Access:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13349
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id oai:ojs.csi.unmsm:article-13349
record_format ojs
institution UNMSM
collection OJS
country Perú
countrycode PE
component Revista
access En linea
databasecode rev-rivep
tag revista
region America del Sur
libraryname Biblioteca de la Facultad de Medicina Veterinaria
language spa
format Digital
author Restrepo-Betancur, Giovanni
Montoya Páez, Juan David
Arboleda Chacón, Lucy
spellingShingle Restrepo-Betancur, Giovanni
Montoya Páez, Juan David
Arboleda Chacón, Lucy
Evaluation of two cryprotectans and three curves of programable freezing in semen cryopreservation of Brycon henni (Pisces: Characidae)
author_facet Restrepo-Betancur, Giovanni
Montoya Páez, Juan David
Arboleda Chacón, Lucy
author_sort Restrepo-Betancur, Giovanni
title Evaluation of two cryprotectans and three curves of programable freezing in semen cryopreservation of Brycon henni (Pisces: Characidae)
title_short Evaluation of two cryprotectans and three curves of programable freezing in semen cryopreservation of Brycon henni (Pisces: Characidae)
title_full Evaluation of two cryprotectans and three curves of programable freezing in semen cryopreservation of Brycon henni (Pisces: Characidae)
title_fullStr Evaluation of two cryprotectans and three curves of programable freezing in semen cryopreservation of Brycon henni (Pisces: Characidae)
title_full_unstemmed Evaluation of two cryprotectans and three curves of programable freezing in semen cryopreservation of Brycon henni (Pisces: Characidae)
title_sort evaluation of two cryprotectans and three curves of programable freezing in semen cryopreservation of brycon henni (pisces: characidae)
description The aim of this study was to evaluate the cryopreservation of Brycon henni semen using three programmable freezing curves and two permeable cryoprotectants. Semen of 80 males were diluted in medium supplemented with ethylene glycol (EG) or dimethylsulfoxide (DMSO), and cryopreserved in semen straws using three programmable freezing curves: slow (66 min, -0.42 °C/min), medium (43.3 min, -0.6 °C/min) and ultra-rapid (7.7 min, -5.19 °C/min). After one month of storage, semen was thawed and motility was evaluated through a class analysis system (SCA®) and sperm vitality (EV) by fluorescence microscopy with SYBR14/IP probes. For statistical analysis, generalized linear models (GLM) were adjusted and means compared by the Tukey test. The medium and ultrarapid velocity curves showed higher and equivalent values for total motility, progressive motility, and linear velocity of sperm (p<0.05). Sperm vitality was better in the medium velocity curve (52.4 ± 8.6%) in comparison with the ultra-rapid (43.0 ± 19.4%) and slow (29.0 ± 11.8%) curves (p<0.05). EG showed greater sperm vitality (p<0.05). It is concluded that freezing of sabaleta semen (B. henni) by a medium velocity programmable freezing curve and ethylene glycol as cryoprotectant allows better results of post-thaw semen quality.
publisher Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publishDate 2017
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13349
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spelling oai:ojs.csi.unmsm:article-133492017-12-19T21:57:14Z Evaluation of two cryprotectans and three curves of programable freezing in semen cryopreservation of Brycon henni (Pisces: Characidae) Evaluación de Dos Crioprotectores y Tres Curvas de Congelación Programable en la Criopreservación de Semen de Brycon henni (Pisces: Characidae) Restrepo-Betancur, Giovanni Montoya Páez, Juan David Arboleda Chacón, Lucy cryopreservation programmable freezing fish spermatozoa sabaleta criopreservación congelación programable peces espermatozoide sabaleta The aim of this study was to evaluate the cryopreservation of Brycon henni semen using three programmable freezing curves and two permeable cryoprotectants. Semen of 80 males were diluted in medium supplemented with ethylene glycol (EG) or dimethylsulfoxide (DMSO), and cryopreserved in semen straws using three programmable freezing curves: slow (66 min, -0.42 °C/min), medium (43.3 min, -0.6 °C/min) and ultra-rapid (7.7 min, -5.19 °C/min). After one month of storage, semen was thawed and motility was evaluated through a class analysis system (SCA®) and sperm vitality (EV) by fluorescence microscopy with SYBR14/IP probes. For statistical analysis, generalized linear models (GLM) were adjusted and means compared by the Tukey test. The medium and ultrarapid velocity curves showed higher and equivalent values for total motility, progressive motility, and linear velocity of sperm (p<0.05). Sperm vitality was better in the medium velocity curve (52.4 ± 8.6%) in comparison with the ultra-rapid (43.0 ± 19.4%) and slow (29.0 ± 11.8%) curves (p<0.05). EG showed greater sperm vitality (p<0.05). It is concluded that freezing of sabaleta semen (B. henni) by a medium velocity programmable freezing curve and ethylene glycol as cryoprotectant allows better results of post-thaw semen quality. El objetivo del presente estudio fue evaluar la criopreservación de semen de Brycon henni, mediante tres curvas de congelación programable y con el empleo de dos crioprotectores permeables. El semen de 30 machos se diluyó en un medio suplementado con etilenglicol (EG) o dimetilsulfóxido (DMSO) y se crioconservó en pajillas de semen, utilizando tres curvas de congelación programable: lenta (66 min, -0.42 ºC/min), media (43.3 min, -0.6 ºC/min) y ultra-rápida (7.7 min, -5.19 ºC/min). Después de un mes de almacenamiento, el semen fue descongelado y se evaluó la movilidad, mediante el sistema de análisis de clase (SCA®) y la vitalidad espermática (VE) mediante microscopía de fluorescencia con las sondas SYBR14/IP. Para el análisis estadístico se ajustaron modelos lineales generalizados (GLM) y las medias se compararon por la prueba de Tukey. Las curvas de velocidad media y ultra-rápida presentaron valores superiores y equivalentes para la movilidad total, la movilidad progresiva y la velocidad lineal de los espermatozoides (p<0.05). La vitalidad espermática fue superior empleando la curva de velocidad media (52.4 ± 8.6%) en relación a las curvas ultra-rápida (43.0 ± 19.4%) y lenta (29.0 ± 11.8%) (p<0.05). El EG presentó una mejor vitalidad espermática (p<0.05). Se concluye que la congelación de semen de sabaleta (B. henni) usando una curva de congelación programable de velocidad media y etilenglicol como crioprotector permite resultados superiores de calidad seminal posdescongelación Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2017-10-11 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article application/pdf https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13349 10.15381/rivep.v28i3.13349 Revista de Investigaciones Veterinarias del Perú; Vol. 28 No. 3 (2017); 597-605 Revista de Investigaciones Veterinarias del Perú; Vol. 28 Núm. 3 (2017); 597-605 1682-3419 1609-9117 spa https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13349/12267 Derechos de autor 2017 Giovanni Restrepo-Betancur, Juan David Montoya Páez, Lucy Arboleda Chacón https://creativecommons.org/licenses/by-nc-sa/4.0

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