Molecular sexing from stools in spectacled bear (Tremarctos ornatus)

Molecular sexing from stools in spectacled bear (Tremarctos ornatus)

The spectacled bear (Tremarctos ornatus) is a vulnerable species because of hunting and destruction of its environment. Also, it is difficult to monitor in the wild hampering the management and research efforts and therefore, the use of non-invasive samples such as faeces becomes a useful tool. The aim of this study was to develop a technique for sexing by the Polymerase Chain Reaction (PCR) using DNA extracted from exfoliated colon cells present in faecal samples. Seventeen samples (from 10 females and 7 males) were collected from bears of known sex reared in captivity in four institutions in Lima Peru. DNA extraction was done by using a specific kit for faecal samples. The concentration of extracted DNA varied between 20 to 30 ng. Using PCR, two gene sequences were evaluated: amelogenin with primers SE47-SE48 and sex-determining region in the Y chromosome (SRY) with primers SRYB5-SRYB3. Sex determination by the amelogenin gene was achieved in all bears (100%), whereas failed in all samples when using the SRY gene primers. It is concluded that there is 100% match with the amelogenin gene between the known and detected sex using the PCR of DNA extracted from faeces.

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Main Authors: Caselli S., Caselli S., Maturrano H., Lenin
Format: Digital revista
Language:spa
Published: Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2016
Online Access:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11641
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spelling oai:ojs.csi.unmsm:article-116412017-02-20T21:35:40Z Molecular sexing from stools in spectacled bear (Tremarctos ornatus) Sexaje Molecular a Partir de Heces en Osos de Anteojos (Tremarctos ornatus) Caselli S., Caselli S. Maturrano H., Lenin spectacled bear sexing amelogenin gene SRY PCR faeces oso de anteojos sexado gen de amelogenina SRY PCR heces The spectacled bear (Tremarctos ornatus) is a vulnerable species because of hunting and destruction of its environment. Also, it is difficult to monitor in the wild hampering the management and research efforts and therefore, the use of non-invasive samples such as faeces becomes a useful tool. The aim of this study was to develop a technique for sexing by the Polymerase Chain Reaction (PCR) using DNA extracted from exfoliated colon cells present in faecal samples. Seventeen samples (from 10 females and 7 males) were collected from bears of known sex reared in captivity in four institutions in Lima Peru. DNA extraction was done by using a specific kit for faecal samples. The concentration of extracted DNA varied between 20 to 30 ng. Using PCR, two gene sequences were evaluated: amelogenin with primers SE47-SE48 and sex-determining region in the Y chromosome (SRY) with primers SRYB5-SRYB3. Sex determination by the amelogenin gene was achieved in all bears (100%), whereas failed in all samples when using the SRY gene primers. It is concluded that there is 100% match with the amelogenin gene between the known and detected sex using the PCR of DNA extracted from faeces. El oso de anteojos (Tremarctos ornatus) se encuentra vulnerable debido a la caza y destrucción de su medio ambiente y es de difícil observación en vida libre, lo que obstaculiza su manejo e investigación, por lo que el uso de muestras no invasivas como heces se convierte en una gran herramienta. El objetivo del presente estudio fue adaptar una técnica de sexado mediante la reacción en cadena de la polimerasa (PCR) de ADN extraído de células exfoliadas del colon presentes en muestras de heces. Se utilizaron 17 muestras (10 de hembras y 7 de machos) de osos de sexo conocido y criados en cautiverio en cuatro instituciones de la zona de Lima, Perú. Para la extracción de ADN se utilizó un kit comercial específico para heces. Se logró extraer de 20 a 30 ng de ADN de cada muestra. Mediante PCR se evaluaron dos secuencias de genes: amelogenina con los cebadores SE47-SE48 y la región determinante del sexo en el cromosoma Y (SRY) con los cebadores SRYB3- SRYB5. Con el gen de amelogenina se logró sexar a los 17 osos (100%), mientras que con el gen SRY no se logró el sexado. Se concluye que con el gen amelogenina hay una coincidencia de 100% entre el sexo conocido y el sexo hallado mediante PCR de ADN extraído de heces. Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2016-06-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article application/pdf https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11641 10.15381/rivep.v27i2.11641 Revista de Investigaciones Veterinarias del Perú; Vol. 27 No. 2 (2016); 252-258 Revista de Investigaciones Veterinarias del Perú; Vol. 27 Núm. 2 (2016); 252-258 1682-3419 1609-9117 spa https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11641/10644 Derechos de autor 2016 Caselli S. Caselli S., Lenin Maturrano H. https://creativecommons.org/licenses/by-nc-sa/4.0
institution UNMSM
collection OJS
country Perú
countrycode PE
component Revista
access En linea
databasecode rev-rivep
tag revista
region America del Sur
libraryname Biblioteca de la Facultad de Medicina Veterinaria
language spa
format Digital
author Caselli S., Caselli S.
Maturrano H., Lenin
spellingShingle Caselli S., Caselli S.
Maturrano H., Lenin
Molecular sexing from stools in spectacled bear (Tremarctos ornatus)
author_facet Caselli S., Caselli S.
Maturrano H., Lenin
author_sort Caselli S., Caselli S.
title Molecular sexing from stools in spectacled bear (Tremarctos ornatus)
title_short Molecular sexing from stools in spectacled bear (Tremarctos ornatus)
title_full Molecular sexing from stools in spectacled bear (Tremarctos ornatus)
title_fullStr Molecular sexing from stools in spectacled bear (Tremarctos ornatus)
title_full_unstemmed Molecular sexing from stools in spectacled bear (Tremarctos ornatus)
title_sort molecular sexing from stools in spectacled bear (tremarctos ornatus)
description The spectacled bear (Tremarctos ornatus) is a vulnerable species because of hunting and destruction of its environment. Also, it is difficult to monitor in the wild hampering the management and research efforts and therefore, the use of non-invasive samples such as faeces becomes a useful tool. The aim of this study was to develop a technique for sexing by the Polymerase Chain Reaction (PCR) using DNA extracted from exfoliated colon cells present in faecal samples. Seventeen samples (from 10 females and 7 males) were collected from bears of known sex reared in captivity in four institutions in Lima Peru. DNA extraction was done by using a specific kit for faecal samples. The concentration of extracted DNA varied between 20 to 30 ng. Using PCR, two gene sequences were evaluated: amelogenin with primers SE47-SE48 and sex-determining region in the Y chromosome (SRY) with primers SRYB5-SRYB3. Sex determination by the amelogenin gene was achieved in all bears (100%), whereas failed in all samples when using the SRY gene primers. It is concluded that there is 100% match with the amelogenin gene between the known and detected sex using the PCR of DNA extracted from faeces.
publisher Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publishDate 2016
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11641
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AT maturranohlenin sexajemolecularapartirdehecesenososdeanteojostremarctosornatus
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