Expression of pro-inflammatory citokines of alpaca (Vicugna pacos) leukocytes induced by extract of macrocyst of Sarcocystis aucheniae

Expression of pro-inflammatory citokines of alpaca (Vicugna pacos) leukocytes induced by extract of macrocyst of Sarcocystis aucheniae

The aim of this research was to determine the expression of proinflammatory cytokines from alpaca leukocytes through the antigenic challenge with macrocyst extract of Sarcocystis aucheniae at various doses and exposure times. Leukocytes in a concentration of 500 000 cells/ml were exposed to concentrations of 0.5, 1, 50, 500 and 1000 ng of macrocyst extract ofS. aucheniae and incubated for 1, 12 and 24 h. The total messenger RNA (mRNA) was extracted for each treatment using Trizol and used to perform real-time RT-PCR with specific primers for cytokine TNF-α and interleukins IL-1α, IL-1β and IL-6. The generated mRNA levels of IL-1α and TNF-α at 1 h were detectable and higher in comparison to the calibrator (leukocytes not exposed to the extract). IL-1β increased at the 1 ng/ml concentration at 24 h showing a negative kinetic expression when compared with the untreated control group. IL-6 was not evidenced by RT-PCR real time. In addition, the leukocytes viability at 1, 12 and 24 h corroborated the toxic effect of the macrocyst extract ofS. aucheniae at high concentrations.

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Main Authors: Hinostroza S., Rocío, Manchego S., Alberto, Sandoval C., Nieves, Chiok C., Kim-Lam, More B., Juan
Format: Digital revista
Language:spa
Published: Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2015
Online Access:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11007
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spelling oai:ojs.csi.unmsm:article-110072020-03-29T23:54:48Z Expression of pro-inflammatory citokines of alpaca (Vicugna pacos) leukocytes induced by extract of macrocyst of Sarcocystis aucheniae Expresión de Citocinas Pro-Inflamatorias de Leucocitos de Alpaca (Vicugna pacos) Inducidos por el Extracto de Macroquistes de Sarcocystis aucheniae Hinostroza S., Rocío Manchego S., Alberto Sandoval C., Nieves Chiok C., Kim-Lam More B., Juan Sarcocystis aucheniae interleukins pro-inflammatory alpaca Sarcocystis aucheniae interleucinas pro-inflamatorias alpaca The aim of this research was to determine the expression of proinflammatory cytokines from alpaca leukocytes through the antigenic challenge with macrocyst extract of Sarcocystis aucheniae at various doses and exposure times. Leukocytes in a concentration of 500 000 cells/ml were exposed to concentrations of 0.5, 1, 50, 500 and 1000 ng of macrocyst extract ofS. aucheniae and incubated for 1, 12 and 24 h. The total messenger RNA (mRNA) was extracted for each treatment using Trizol and used to perform real-time RT-PCR with specific primers for cytokine TNF-α and interleukins IL-1α, IL-1β and IL-6. The generated mRNA levels of IL-1α and TNF-α at 1 h were detectable and higher in comparison to the calibrator (leukocytes not exposed to the extract). IL-1β increased at the 1 ng/ml concentration at 24 h showing a negative kinetic expression when compared with the untreated control group. IL-6 was not evidenced by RT-PCR real time. In addition, the leukocytes viability at 1, 12 and 24 h corroborated the toxic effect of the macrocyst extract ofS. aucheniae at high concentrations. El objetivo del trabajo fue determinar la expresión de citocinas pro-inflamatorias de leucocitos de alpaca mediante el enfrentamiento antigénico de extracto de macroquistes de Sarcocystis aucheniae a diversas dosis y tiempos de exposición. Se empleó una concentración de leucocitos de 500 000/ml expuestos a 0.5, 1, 50, 500 y 1000 ng de extracto de macroquistes de S. aucheniae e incubados por 1, 12 y 24 h. Se extrajo los ARN mensajeros (ARNm) totales de cada tratamiento con Trizol y se utilizaron en una RT-PCR tiempo real con cebadores específicos de la citosina TNF-α e interleucinas IL-1α, IL-1β e IL-6. Se observó que los niveles de ARNm de IL-1α y TNF-α generados a 1 h de incubación eran detectables y comparativamente elevados con respecto al calibrador (leucocitos no expuestos a extracto). La IL-1β se encontró incrementada en la concentración de 1 ng/ml a las 24 h, mostrando una cinética de expresión negativa en comparación con el control no tratado. La IL-6 no se evidenció mediante RT-PCR tiempo real. Asimismo, se realizó la observación de viabilidad leucocitaria a los tiempos de 1, 12 y 24 h permitiendo corroborar el efecto tóxico del extracto de macroquistes de S. aucheniae a altas concentraciones. Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2015-06-03 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article application/pdf https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11007 10.15381/rivep.v26i2.11007 Revista de Investigaciones Veterinarias del Perú; Vol. 26 No. 2 (2015); 328-341 Revista de Investigaciones Veterinarias del Perú; Vol. 26 Núm. 2 (2015); 328-341 1682-3419 1609-9117 spa https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11007/10063 Derechos de autor 2015 Rocío Hinostroza S., Alberto Manchego S., Nieves Sandoval C., Kim-Lam Chiok C., Juan More B. https://creativecommons.org/licenses/by-nc-sa/4.0
institution UNMSM
collection OJS
country Perú
countrycode PE
component Revista
access En linea
databasecode rev-rivep
tag revista
region America del Sur
libraryname Biblioteca de la Facultad de Medicina Veterinaria
language spa
format Digital
author Hinostroza S., Rocío
Manchego S., Alberto
Sandoval C., Nieves
Chiok C., Kim-Lam
More B., Juan
spellingShingle Hinostroza S., Rocío
Manchego S., Alberto
Sandoval C., Nieves
Chiok C., Kim-Lam
More B., Juan
Expression of pro-inflammatory citokines of alpaca (Vicugna pacos) leukocytes induced by extract of macrocyst of Sarcocystis aucheniae
author_facet Hinostroza S., Rocío
Manchego S., Alberto
Sandoval C., Nieves
Chiok C., Kim-Lam
More B., Juan
author_sort Hinostroza S., Rocío
title Expression of pro-inflammatory citokines of alpaca (Vicugna pacos) leukocytes induced by extract of macrocyst of Sarcocystis aucheniae
title_short Expression of pro-inflammatory citokines of alpaca (Vicugna pacos) leukocytes induced by extract of macrocyst of Sarcocystis aucheniae
title_full Expression of pro-inflammatory citokines of alpaca (Vicugna pacos) leukocytes induced by extract of macrocyst of Sarcocystis aucheniae
title_fullStr Expression of pro-inflammatory citokines of alpaca (Vicugna pacos) leukocytes induced by extract of macrocyst of Sarcocystis aucheniae
title_full_unstemmed Expression of pro-inflammatory citokines of alpaca (Vicugna pacos) leukocytes induced by extract of macrocyst of Sarcocystis aucheniae
title_sort expression of pro-inflammatory citokines of alpaca (vicugna pacos) leukocytes induced by extract of macrocyst of sarcocystis aucheniae
description The aim of this research was to determine the expression of proinflammatory cytokines from alpaca leukocytes through the antigenic challenge with macrocyst extract of Sarcocystis aucheniae at various doses and exposure times. Leukocytes in a concentration of 500 000 cells/ml were exposed to concentrations of 0.5, 1, 50, 500 and 1000 ng of macrocyst extract ofS. aucheniae and incubated for 1, 12 and 24 h. The total messenger RNA (mRNA) was extracted for each treatment using Trizol and used to perform real-time RT-PCR with specific primers for cytokine TNF-α and interleukins IL-1α, IL-1β and IL-6. The generated mRNA levels of IL-1α and TNF-α at 1 h were detectable and higher in comparison to the calibrator (leukocytes not exposed to the extract). IL-1β increased at the 1 ng/ml concentration at 24 h showing a negative kinetic expression when compared with the untreated control group. IL-6 was not evidenced by RT-PCR real time. In addition, the leukocytes viability at 1, 12 and 24 h corroborated the toxic effect of the macrocyst extract ofS. aucheniae at high concentrations.
publisher Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publishDate 2015
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11007
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