Sanitary evaluation of swine herds in peri-urban farming of Uriburu, La Pampa
The objective of this study was to determine the presence antibodies of Brucella suis and of Aujeszky’s Disease Virus in swine herds located in peri-urban limits of Uriburu (La Pampa). A total of 290 breeding animals from 31 swine farmers were serologically sampled. For the detection of agents against Brucellosis, the serum lot was processed by the agglutination technique with Buffered Plate Antigen (BPA) and Fluorescent Polarization (FPA) according to Nielsen et al.,1999. For Aujeszky’s Disease Virus, a commercial blocking ELISA kit was used for the detection of specific antibodies from Aujeszky’s Disease Virus glycoprotein E (CIVTEST — Laboratorios HIPRA S. A, Girona, Spain). Only 1.03% of sampled animals resulted positive for Brucella suis distributed in 3 farms (9.7%). On the other hand, 16 positive cases for Aujeszky’s Disease Virus (5.52%) were detected in 8 farms (25.8%). Only 2 farms share positive cases for Brucella suis and Aujeszky’s Disease Virus, which reduces to 9 farms (29%) that have been affected by either of these two diseases. Due to the relevant information obtained, the need to continue with the research is highlighted, expanding the sampling to other towns within La Pampa province as well as to determine the presence of other etiological agents.
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Murcia, Vanina Nerea Beneitez, Horacio Adrián Lorda, Hector Oscar Gimenez, Hugo Fort, Marcelo |
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Murcia, Vanina Nerea Beneitez, Horacio Adrián Lorda, Hector Oscar Gimenez, Hugo Fort, Marcelo Sanitary evaluation of swine herds in peri-urban farming of Uriburu, La Pampa |
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Murcia, Vanina Nerea Beneitez, Horacio Adrián Lorda, Hector Oscar Gimenez, Hugo Fort, Marcelo |
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Murcia, Vanina Nerea |
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Sanitary evaluation of swine herds in peri-urban farming of Uriburu, La Pampa |
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Sanitary evaluation of swine herds in peri-urban farming of Uriburu, La Pampa |
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Sanitary evaluation of swine herds in peri-urban farming of Uriburu, La Pampa |
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Sanitary evaluation of swine herds in peri-urban farming of Uriburu, La Pampa |
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Sanitary evaluation of swine herds in peri-urban farming of Uriburu, La Pampa |
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sanitary evaluation of swine herds in peri-urban farming of uriburu, la pampa |
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The objective of this study was to determine the presence antibodies of Brucella suis and of Aujeszky’s Disease Virus in swine herds located in peri-urban limits of Uriburu (La Pampa). A total of 290 breeding animals from 31 swine farmers were serologically sampled. For the detection of agents against Brucellosis, the serum lot was processed by the agglutination technique with Buffered Plate Antigen (BPA) and Fluorescent Polarization (FPA) according to Nielsen et al.,1999. For Aujeszky’s Disease Virus, a commercial blocking ELISA kit was used for the detection of specific antibodies from Aujeszky’s Disease Virus glycoprotein E (CIVTEST — Laboratorios HIPRA S. A, Girona, Spain). Only 1.03% of sampled animals resulted positive for Brucella suis distributed in 3 farms (9.7%). On the other hand, 16 positive cases for Aujeszky’s Disease Virus (5.52%) were detected in 8 farms (25.8%). Only 2 farms share positive cases for Brucella suis and Aujeszky’s Disease Virus, which reduces to 9 farms (29%) that have been affected by either of these two diseases. Due to the relevant information obtained, the need to continue with the research is highlighted, expanding the sampling to other towns within La Pampa province as well as to determine the presence of other etiological agents. |
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oai:ojs.cerac.unlpam.edu.ar:article-65722022-09-05T16:22:11Z Sanitary evaluation of swine herds in peri-urban farming of Uriburu, La Pampa Evaluación sanitaria en porcinos de establecimientos periurbanos de la localidad de Uriburu, La Pampa Avaliação sanitária de rebanhos suínos na pecuária periurbana de Uriburu, La Pampa Murcia, Vanina Nerea Beneitez, Horacio Adrián Lorda, Hector Oscar Gimenez, Hugo Fort, Marcelo Brucella suis Aujeszky´s disease virus swine health Brucella suis virus de la Enfermedad de Aujeszky sanidad porcina Brucella suis virus da doença de Aujeszky saúde suína The objective of this study was to determine the presence antibodies of Brucella suis and of Aujeszky’s Disease Virus in swine herds located in peri-urban limits of Uriburu (La Pampa). A total of 290 breeding animals from 31 swine farmers were serologically sampled. For the detection of agents against Brucellosis, the serum lot was processed by the agglutination technique with Buffered Plate Antigen (BPA) and Fluorescent Polarization (FPA) according to Nielsen et al.,1999. For Aujeszky’s Disease Virus, a commercial blocking ELISA kit was used for the detection of specific antibodies from Aujeszky’s Disease Virus glycoprotein E (CIVTEST — Laboratorios HIPRA S. A, Girona, Spain). Only 1.03% of sampled animals resulted positive for Brucella suis distributed in 3 farms (9.7%). On the other hand, 16 positive cases for Aujeszky’s Disease Virus (5.52%) were detected in 8 farms (25.8%). Only 2 farms share positive cases for Brucella suis and Aujeszky’s Disease Virus, which reduces to 9 farms (29%) that have been affected by either of these two diseases. Due to the relevant information obtained, the need to continue with the research is highlighted, expanding the sampling to other towns within La Pampa province as well as to determine the presence of other etiological agents. El objetivo de este estudio fue determinar la presencia de anticuerpos de Brucella suis y del Virus de la Enfermedad de Aujeszky en piaras de la localidad de Uriburu (La Pampa). Se tomaron muestras de sangre en 290 animales reproductores, de 31 productores porcinos. Para la detección de agentes contra Brucelosis los sueros fueron procesados por la técnica de aglutinación con antígeno tamponado en placa (BPA) y polarización fluorescente (FPA) según Nielsen et al.1999. Para Virus de la Enfermedad de Aujeszky se utilizó un kit comercial de ELISA de bloqueo, para la detección de anticuerpos específicos para la glicoproteína E del Virus de la Enfermedad de Aujeszky (CIVTEST— Laboratorios HIPRA S.A Girona, España). El 1,03 % de los animales muestreados resultaron positivos a Brucella suis distribuidos en 3 establecimientos (9,7%). Por otro lado, se detectaron 16 casos positivos para Virus de la Enfermedad de Aujeszky (5,52 %) en 8 establecimientos (25,8%). Solo 2 establecimientos comparten casos positivos para Brucella suis y Virus de la Enfermedad de Aujeszky, lo que reduce a 9 los establecimientos (29%) que han visto afectados por alguna de estas dos enfermedades. Debida a la información relevante obtenida, se remarca la necesidad de continuar con la investigación, ampliando el muestreo a otras localidades pampeanas como así también determinar la presencia de otros agentes etiológicos. O objetivo deste estudo foi determinar a presença de anticorpos de Brucella suis e de virus da doença de Aujeszky em rebanhos do município dos agentes contra a Brucelose, os soros foram processados pela técnica de aglutinação com antígeno de placa tamponada (BPA) e polarização fluorescente (FPA) de acordo com Nielsen et al., (1999). Para o virus da doença de Aujeszky, um kit comercial de bloqueio de ELISA foi usado para detectar anticorpos específicos para a glicoproteína E VA (CIVTEST - Laboratorios HIPRA S.A Girona, Espanha). 1,03% dos animais amostrados foram positivos para Brucella suis distribuídos em 3 estabelecimentos (9,7%). Por outro lado, foram detectados 16 casos positivos para o vírus da doença de Aujeszky (5,52%) em 8 estabelecimentos (25,8%). Apenas 2 estabelecimentos compartilham casos positivos para Brucella suis e vírus da doença de Aujeszky, o que reduz para 9 estabelecimentos (29%) que foram afetados por qualquer uma das duas doenças. Pelas informações relevantes obtidas, destaca-se a necessidade de prosseguir com a investigação, ampliando a amostragem para outras localidades pampeanas, bem como determinando a presença de outros agentes etiológicos. de Uriburu (La Pampa). Amostras de sangue foram coletadas de 290 animais reprodutores de 31 produtores de suínos. Para a detecção dos agentes contra a Brucelose, os soros foram processados pela técnicade aglutinação com antígeno de placa tamponada (BPA) e polarização fluorescente (FPA) de acordo com Nielsen et al., (1999). Para o virus da doença de Aujeszky, um kit comercial de bloqueio de ELISA foi usado para detectar anticorpos específicos para a glicoproteína E VA (CIVTEST - Laboratorios HIPRA S.A Girona, Espanha). 1,03% dos animais amostrados foram positivos para Brucella suis distribuídos em 3 estabelecimentos (9,7%). Por outro lado, foram detectados 16 casos positivos para o vírus da doença de Aujeszky (5,52%) em 8 estabelecimentos (25,8%). Apenas 2 estabelecimentos compartilham casos positivos para Brucella suis e vírus da doença de Aujeszky, o que reduz para 9 estabelecimentos (29%) que foram afetados por qualquer uma das duas doenças. Pelas informações relevantes obtidas, destaca-se a necessidade de prosseguir com a investigação, ampliando a amostragem para outras localidades pampeanas, bem como determinando a presença de outros agentes etiológicos.290 animais reprodutores de 31 produtores de suínos. Para a detecção EdUNLPam 2022-03-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://cerac.unlpam.edu.ar/index.php/veterinaria/article/view/6572 10.19137/cienvet202224103 Ciencia Veterinaria; Vol. 24 Núm. 1 (2022): enero - junio; 47 - 55 1853-8495 1515-1883 spa https://cerac.unlpam.edu.ar/index.php/veterinaria/article/view/6572/7184 https://cerac.unlpam.edu.ar/index.php/veterinaria/article/view/6572/7205 https://cerac.unlpam.edu.ar/index.php/veterinaria/article/view/6572/7260 Derechos de autor 2022 Ciencia Veterinaria https://creativecommons.org/licenses/by-nc-sa/4.0 |