Micropropagation of Achyrocline flaccida (Weinm.) DC. in liquid culture media
Achyrocline flaccida, known by the popular name of ‘Marcela’, is a plant native from Uruguay, south of Brazil, Argentina and Paraguay, frequently found in sandy and stony ground. Its productive interest lies in Marcela´s content of high levels of quercetin, a flavonoid with important antioxidant and cytoprotective properties. In order to increase the propagation scale of the species, the multiplication in liquid medium employing bioreactors was evaluated. Bioreactors represent an efficient plant micropropagation system, avoiding intensive manipulation and reducing costs. Hyperhydricity represents the main limitation of the system. Submerged tissues suffer stress, and the plants obtained show different anatomical and physiological anomalies that may affect their survival ex vitro. Our objective was to optimize a system for the production of A. flaccida microplants in bioreactors, which could allow the scaling up of production. The effect of phloroglucinol to prevent hyperhydricity promoting lignin biosynthesis was assessed, and confirmed with light microscopy. For all treatments multiplication rate was higher than that employing solid medium, with a maximum of 11 micro cuttings per bud in two months. Rooting and survival ex vitro was close to 100 %. These results represent an effective reduction of costs as less manual labor and media components are required. An efficient system has been developed which will contribute to increase the stock of good quality plant material available.
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Coeditada entre Facultad de Agronomía - Udelar y el Instituto Nacional de Investigación Agropecuaria (INIA)
2010
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| Online Access: | https://agrocienciauruguay.uy/index.php/agrociencia/article/view/633 |
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Ross, Silvia Castillo, Alicia |
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Ross, Silvia Castillo, Alicia Micropropagation of Achyrocline flaccida (Weinm.) DC. in liquid culture media |
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Ross, Silvia Castillo, Alicia |
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Ross, Silvia |
| title |
Micropropagation of Achyrocline flaccida (Weinm.) DC. in liquid culture media |
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Micropropagation of Achyrocline flaccida (Weinm.) DC. in liquid culture media |
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Micropropagation of Achyrocline flaccida (Weinm.) DC. in liquid culture media |
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Micropropagation of Achyrocline flaccida (Weinm.) DC. in liquid culture media |
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Micropropagation of Achyrocline flaccida (Weinm.) DC. in liquid culture media |
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micropropagation of achyrocline flaccida (weinm.) dc. in liquid culture media |
| description |
Achyrocline flaccida, known by the popular name of ‘Marcela’, is a plant native from Uruguay, south of Brazil, Argentina and Paraguay, frequently found in sandy and stony ground. Its productive interest lies in Marcela´s content of high levels of quercetin, a flavonoid with important antioxidant and cytoprotective properties. In order to increase the propagation scale of the species, the multiplication in liquid medium employing bioreactors was evaluated. Bioreactors represent an efficient plant micropropagation system, avoiding intensive manipulation and reducing costs. Hyperhydricity represents the main limitation of the system. Submerged tissues suffer stress, and the plants obtained show different anatomical and physiological anomalies that may affect their survival ex vitro. Our objective was to optimize a system for the production of A. flaccida microplants in bioreactors, which could allow the scaling up of production. The effect of phloroglucinol to prevent hyperhydricity promoting lignin biosynthesis was assessed, and confirmed with light microscopy. For all treatments multiplication rate was higher than that employing solid medium, with a maximum of 11 micro cuttings per bud in two months. Rooting and survival ex vitro was close to 100 %. These results represent an effective reduction of costs as less manual labor and media components are required. An efficient system has been developed which will contribute to increase the stock of good quality plant material available. |
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Coeditada entre Facultad de Agronomía - Udelar y el Instituto Nacional de Investigación Agropecuaria (INIA) |
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2010 |
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https://agrocienciauruguay.uy/index.php/agrociencia/article/view/633 |
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AT rosssilvia micropropagationofachyroclineflaccidaweinmdcinliquidculturemedia AT castilloalicia micropropagationofachyroclineflaccidaweinmdcinliquidculturemedia AT rosssilvia micropropagaciondeachyroclineflaccidaweinmdcenmediosdecultivoliquidos AT castilloalicia micropropagaciondeachyroclineflaccidaweinmdcenmediosdecultivoliquidos |
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oai:oai.agrocienciauruguay.uy:article-6332023-12-26T16:39:10Z Micropropagation of Achyrocline flaccida (Weinm.) DC. in liquid culture media Micropropagación de Achyrocline flaccida (Weinm.) DC. en medios de cultivo líquidos Ross, Silvia Castillo, Alicia bioreactors hyperhydricity in vitro culture phloroglucinol biorreactoreses cultivo in vitro hiperhidricidad floroglucinol Achyrocline flaccida, known by the popular name of ‘Marcela’, is a plant native from Uruguay, south of Brazil, Argentina and Paraguay, frequently found in sandy and stony ground. Its productive interest lies in Marcela´s content of high levels of quercetin, a flavonoid with important antioxidant and cytoprotective properties. In order to increase the propagation scale of the species, the multiplication in liquid medium employing bioreactors was evaluated. Bioreactors represent an efficient plant micropropagation system, avoiding intensive manipulation and reducing costs. Hyperhydricity represents the main limitation of the system. Submerged tissues suffer stress, and the plants obtained show different anatomical and physiological anomalies that may affect their survival ex vitro. Our objective was to optimize a system for the production of A. flaccida microplants in bioreactors, which could allow the scaling up of production. The effect of phloroglucinol to prevent hyperhydricity promoting lignin biosynthesis was assessed, and confirmed with light microscopy. For all treatments multiplication rate was higher than that employing solid medium, with a maximum of 11 micro cuttings per bud in two months. Rooting and survival ex vitro was close to 100 %. These results represent an effective reduction of costs as less manual labor and media components are required. An efficient system has been developed which will contribute to increase the stock of good quality plant material available. La ‘Marcela’ (Achyrocline flaccida) es una planta nativa de Uruguay, sur de Brasil, Argentina y Paraguay, frecuente en arenales y sierras. Su interés productivo radica en el elevado contenido de quercetina que presenta, con importantes propiedades antioxidantes y citoprotectoras. Con el fin de incrementar la escala de propagación de esta especie, se evaluó su multiplicación en medio de cultivo líquido empleando biorreactoreses. Los biorreactoreses constituyen un sistema de micropropagación de plantas rápido y eficiente, evitando la manipulación intensiva y disminuyendo costos. La hiperhidricidad representa la principal limitante de estos sistemas. Los tejidos sumergidos sufren cambios que afectan la anatomía y fisiología de las plantas obtenidas y por lo tanto su sobrevivencia. El objetivo de este trabajo fue optimizar un sistema de producción de microplantas de A. flaccida, en biorreactoreses para incrementar la escala de producción. Se evaluó el efecto del floroglucinol por su acción antivitrificante como promotor de la biosíntesis de lignina, confirmándose mediante cortes histológicos de las plantas obtenidas. En todos los tratamientos realizados se logró una mayor tasa de multiplicación que cuando se emplearon medios sólidos, obteniéndose un máximo de 11 microestacas por yema en dos meses. Los porcentajes de enraizamiento y sobrevivencia ex vitro de las microestacas fueron cercanos al 100 %. Estos resultados permiten lograr una reducción efectiva de costos, ya que se requiere menos mano de obra y disminuyen los costos de insumos. Con este trabajo se ajustó un sistema de propagación eficiente que contribuirá a incrementar el stock de plantas disponibles, de buena calidad. Coeditada entre Facultad de Agronomía - Udelar y el Instituto Nacional de Investigación Agropecuaria (INIA) 2010-06-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://agrocienciauruguay.uy/index.php/agrociencia/article/view/633 10.31285/AGRO.14.633 Agrociencia Uruguay; Vol. 14 No. 1 (2010); 1- 7 Agrociencia Uruguay; Vol. 14 Núm. 1 (2010); 1- 7 Agrociencia Uruguay; v. 14 n. 1 (2010); 1- 7 2730-5066 spa https://agrocienciauruguay.uy/index.php/agrociencia/article/view/633/655 Copyright (c) 2021 Agrociencia Uruguay https://creativecommons.org/licenses/by/4.0 |