Development of a Microsatellite Multiplex for Paternity Testing in Uruguayan Corriedale Sheep

Development of a Microsatellite Multiplex for Paternity Testing in Uruguayan Corriedale Sheep

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The Corriedale breed represents 70% of Uruguayan sheep stock, therefore it is important to guarantee accuracy in paternity assessment through DNA analysis, to contribute to genealogy recording and selection programs for economically relevant traits of the breed. In this study, a multiplex of 10 microsatellites (Short Tandem Repeats, STR) recommended from the ISAG (International Society of Animal Genetics) panel was developed. With this multiplex, a population of 156 Corriedale sheep was analyzed. The aim of this study was to evaluate the multiplex according to genetic variability of allele frequencies, for assigning parents through the their Probability of Exclusion (PE) values. The STR showed a relatively high variability according to the average Heterozygosity Index (HObs=0.661), the number of alleles observed (N=8.6), and the Polymorphic Information Content (PIC=0.614). In four ot the 10 STR (FCB20, TGLA53, MCM527 and MCM130) there was a loss of Hardy-Weinberg equilibrium due to the significant decrease of heterozygotes, with a consequent reduction of genetic variability. As a result, combined exclusion probability presented a value of 0.960. We conclude that in order to improve the accuracy in paternity diagnostic the multiplex could include a greater number of STR, given the low genetic variability present in the parents.

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Main Authors: Peraza, Pablo, Rincón, Gonzalo, Ravagnolo, Olga, Dalla Rizza, Marco, Kelly, Lucy
Format: Digital revista
Language:spa
Published: Coeditada entre Facultad de Agronomía - Udelar y el Instituto Nacional de Investigación Agropecuaria (INIA) 2013
Online Access:https://agrocienciauruguay.uy/index.php/agrociencia/article/view/523
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spelling oai:oai.agrocienciauruguay.uy:article-5232022-12-21T15:05:39Z Development of a Microsatellite Multiplex for Paternity Testing in Uruguayan Corriedale Sheep Desarrollo de un multiplex de microsatélites para diagnóstico de paternidad en ovinos Corriedale del Uruguay Peraza, Pablo Rincón, Gonzalo Ravagnolo, Olga Dalla Rizza, Marco Kelly, Lucy DNA analysis sheep STR tipificación ADN ovinos STR The Corriedale breed represents 70% of Uruguayan sheep stock, therefore it is important to guarantee accuracy in paternity assessment through DNA analysis, to contribute to genealogy recording and selection programs for economically relevant traits of the breed. In this study, a multiplex of 10 microsatellites (Short Tandem Repeats, STR) recommended from the ISAG (International Society of Animal Genetics) panel was developed. With this multiplex, a population of 156 Corriedale sheep was analyzed. The aim of this study was to evaluate the multiplex according to genetic variability of allele frequencies, for assigning parents through the their Probability of Exclusion (PE) values. The STR showed a relatively high variability according to the average Heterozygosity Index (HObs=0.661), the number of alleles observed (N=8.6), and the Polymorphic Information Content (PIC=0.614). In four ot the 10 STR (FCB20, TGLA53, MCM527 and MCM130) there was a loss of Hardy-Weinberg equilibrium due to the significant decrease of heterozygotes, with a consequent reduction of genetic variability. As a result, combined exclusion probability presented a value of 0.960. We conclude that in order to improve the accuracy in paternity diagnostic the multiplex could include a greater number of STR, given the low genetic variability present in the parents. En el Uruguay la raza Corriedale representa el 70% del stock ovino, por lo cual es importante garantizar la exactitud al realizar un diagnóstico de paternidades mediante tipificación de ADN, con el fin de asistir a los registros genealógicos y a los programas de selección para las características de importancia económica realizados en dicha raza. En el presente trabajo se desarrolló un multiplex de 10 microsatélites (Short Tandem Repeats, STR) recomendados del panel de la ISAG (International Society of Animal Genetics). Con este multiplex se analizó una población de 156 ovinos de la raza Corriedale del Uruguay. El objetivo del presente trabajo fue evaluar dicho multiplex de acuerdo a variabilidad genética de las frecuencias alélicas para la asignación de progenitores mediante los valores de su Probabilidad de Exclusión (PE). Los STR presentaron una variabilidad relativamente alta de acuerdo al Índice de Heterocigosidad promedio (HObs=0,661), al número de alelos observados (N=8,6) y al Contenido de Información Polimórfica (PIC=0,614). Se observó en cuatro de los 10 STR utilizados (FCB20, TGLA53, MCM527 y MCM130) una pérdida de equilibrio Hardy-Weinberg debido a la disminución significativa de heterocigotos, con la consiguiente reducción de variabilidad genética. Como consecuencia la Probabilidad de Exclusión combinada en dicha población presentó un valor de 0,960. Concluimos que para mejorar la certeza en el diagnóstico de paternidad se podría incluir un mayor número de STR en el multiplex, dada la baja variabilidad genética presente en los padres. Coeditada entre Facultad de Agronomía - Udelar y el Instituto Nacional de Investigación Agropecuaria (INIA) 2013-06-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://agrocienciauruguay.uy/index.php/agrociencia/article/view/523 10.31285/AGRO.17.523 Agrociencia Uruguay; Vol. 17 No. 1 (2013); 114- 119 Agrociencia Uruguay; Vol. 17 Núm. 1 (2013); 114- 119 Agrociencia Uruguay; v. 17 n. 1 (2013); 114- 119 2730-5066 spa https://agrocienciauruguay.uy/index.php/agrociencia/article/view/523/489 Copyright (c) 2021 Agrociencia Uruguay http://creativecommons.org/licenses/by/4.0
institution UDELAR
collection OJS
country Uruguay
countrycode UY
component Revista
access En linea
databasecode rev-agrociencia-uy
tag revista
region America del Sur
libraryname Biblioteca de la Facultad de Agronomía de la UDELAR de UY
language spa
format Digital
author Peraza, Pablo
Rincón, Gonzalo
Ravagnolo, Olga
Dalla Rizza, Marco
Kelly, Lucy
spellingShingle Peraza, Pablo
Rincón, Gonzalo
Ravagnolo, Olga
Dalla Rizza, Marco
Kelly, Lucy
Development of a Microsatellite Multiplex for Paternity Testing in Uruguayan Corriedale Sheep
author_facet Peraza, Pablo
Rincón, Gonzalo
Ravagnolo, Olga
Dalla Rizza, Marco
Kelly, Lucy
author_sort Peraza, Pablo
title Development of a Microsatellite Multiplex for Paternity Testing in Uruguayan Corriedale Sheep
title_short Development of a Microsatellite Multiplex for Paternity Testing in Uruguayan Corriedale Sheep
title_full Development of a Microsatellite Multiplex for Paternity Testing in Uruguayan Corriedale Sheep
title_fullStr Development of a Microsatellite Multiplex for Paternity Testing in Uruguayan Corriedale Sheep
title_full_unstemmed Development of a Microsatellite Multiplex for Paternity Testing in Uruguayan Corriedale Sheep
title_sort development of a microsatellite multiplex for paternity testing in uruguayan corriedale sheep
description The Corriedale breed represents 70% of Uruguayan sheep stock, therefore it is important to guarantee accuracy in paternity assessment through DNA analysis, to contribute to genealogy recording and selection programs for economically relevant traits of the breed. In this study, a multiplex of 10 microsatellites (Short Tandem Repeats, STR) recommended from the ISAG (International Society of Animal Genetics) panel was developed. With this multiplex, a population of 156 Corriedale sheep was analyzed. The aim of this study was to evaluate the multiplex according to genetic variability of allele frequencies, for assigning parents through the their Probability of Exclusion (PE) values. The STR showed a relatively high variability according to the average Heterozygosity Index (HObs=0.661), the number of alleles observed (N=8.6), and the Polymorphic Information Content (PIC=0.614). In four ot the 10 STR (FCB20, TGLA53, MCM527 and MCM130) there was a loss of Hardy-Weinberg equilibrium due to the significant decrease of heterozygotes, with a consequent reduction of genetic variability. As a result, combined exclusion probability presented a value of 0.960. We conclude that in order to improve the accuracy in paternity diagnostic the multiplex could include a greater number of STR, given the low genetic variability present in the parents.
publisher Coeditada entre Facultad de Agronomía - Udelar y el Instituto Nacional de Investigación Agropecuaria (INIA)
publishDate 2013
url https://agrocienciauruguay.uy/index.php/agrociencia/article/view/523
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