Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina

Alfalfa is an important forage crop in Argentina, with a large area dedicated to its cultivation. However, plant diseases are a common cause of loss because they limit productivity and reduce forage quality. Anthracnose, caused by the Colletotrichum species, is one of the most important diseases affecting alfalfa worldwide. In July 2018, alfalfa plants with anthracnose symptoms were collected in commercial fields of Cordoba province, Argentina. Initially, infected plants showed small, pale gray lesions on stems, which turned oval and developed irregular dark brown/black borders as they enlarged. Three fungal isolates were obtained from the necrotic tissues of stems and single-spore purified. Based on the morphology and multilocus phylogenetic analysis, combined sequences of the partial glyceraldehyde-3-phosphate dehydrogenase (GAPDH), histone H3 (HIS3) and actin (ACT) genes, the three isolates were identified as Colletotrichum americae-borealis. Pathogenicity testing showed these isolates were pathogenic to alfalfa and Koch's postulates were verified by re-isolating the pathogen.

Saved in:
Bibliographic Details
Main Authors: Bernanrdi Lima, Nelson, Conforto, Erica Cinthia, Serri, Dannae Lilia, Campilongo Mancilla, Ernesto Javier, Stenglein, Sebastian, Vargas Gil, Silvina
Format: info:ar-repo/semantics/artículo biblioteca
Language:eng
Published: Elsevier 2021-06-06
Subjects:Medicago Sativa, Colletotrichum, Argentina, Anthracnosis, Phylogeny, Filogenia, Antracnosis, Molecular Phylogeny, Plant Pathogen, Alfalfa, Lucerne, Córdoba, Argentina,
Online Access:http://hdl.handle.net/20.500.12123/9785
https://www.sciencedirect.com/science/article/pii/S0261219421002027
https://doi.org/10.1016/j.cropro.2021.105732
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:localhost:20.500.12123-9785
record_format koha
spelling oai:localhost:20.500.12123-97852021-07-12T17:14:19Z Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina Bernanrdi Lima, Nelson Conforto, Erica Cinthia Serri, Dannae Lilia Campilongo Mancilla, Ernesto Javier Stenglein, Sebastian Vargas Gil, Silvina Medicago Sativa Colletotrichum Argentina Anthracnosis Phylogeny Filogenia Antracnosis Molecular Phylogeny Plant Pathogen Alfalfa Lucerne Córdoba, Argentina Alfalfa is an important forage crop in Argentina, with a large area dedicated to its cultivation. However, plant diseases are a common cause of loss because they limit productivity and reduce forage quality. Anthracnose, caused by the Colletotrichum species, is one of the most important diseases affecting alfalfa worldwide. In July 2018, alfalfa plants with anthracnose symptoms were collected in commercial fields of Cordoba province, Argentina. Initially, infected plants showed small, pale gray lesions on stems, which turned oval and developed irregular dark brown/black borders as they enlarged. Three fungal isolates were obtained from the necrotic tissues of stems and single-spore purified. Based on the morphology and multilocus phylogenetic analysis, combined sequences of the partial glyceraldehyde-3-phosphate dehydrogenase (GAPDH), histone H3 (HIS3) and actin (ACT) genes, the three isolates were identified as Colletotrichum americae-borealis. Pathogenicity testing showed these isolates were pathogenic to alfalfa and Koch's postulates were verified by re-isolating the pathogen. Instituto de Patología Vegetal Fil: Bernardi Lima, Nelson. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Bernardi Lima, Nelson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Conforto, Erica Cinthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Serri, Dannae Lilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Serri, Dannae Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Campilongo Mancilla, Ernesto Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Campilongo Mancilla, Ernesto Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Stenglein, Sebastian. Laboratorio de Biología Functional y Biotecnología (BIOLAB). Unidad Ejecutora Instituto de Investigaciones en Biodiversidad y Biotecnología (INBIOTEC); Argentina Fil: Vargas Gil, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Vargas Gil, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina 2021-07-12T16:43:02Z 2021-07-12T16:43:02Z 2021-06-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9785 https://www.sciencedirect.com/science/article/pii/S0261219421002027 0261-2194 https://doi.org/10.1016/j.cropro.2021.105732 eng info:eu-repograntAgreement/INTA/2019-PD-E4-I090-001/2019-PD-E4-I090-001/AR./Análisis de patosistemas en cultivos agrícolas y especies forestales. Caracterización de sus componentes info:eu-repo/semantics/restrictedAccess application/pdf Córdoba .......... (province) (World, South America, Argentina) 1001169 Elsevier Crop Protection 148 : 105732 ( 2021)
institution INTA AR
collection DSpace
country Argentina
countrycode AR
component Bibliográfico
access En linea
databasecode dig-inta-ar
tag biblioteca
region America del Sur
libraryname Biblioteca Central del INTA Argentina
language eng
topic Medicago Sativa
Colletotrichum
Argentina
Anthracnosis
Phylogeny
Filogenia
Antracnosis
Molecular Phylogeny
Plant Pathogen
Alfalfa
Lucerne
Córdoba, Argentina
Medicago Sativa
Colletotrichum
Argentina
Anthracnosis
Phylogeny
Filogenia
Antracnosis
Molecular Phylogeny
Plant Pathogen
Alfalfa
Lucerne
Córdoba, Argentina
spellingShingle Medicago Sativa
Colletotrichum
Argentina
Anthracnosis
Phylogeny
Filogenia
Antracnosis
Molecular Phylogeny
Plant Pathogen
Alfalfa
Lucerne
Córdoba, Argentina
Medicago Sativa
Colletotrichum
Argentina
Anthracnosis
Phylogeny
Filogenia
Antracnosis
Molecular Phylogeny
Plant Pathogen
Alfalfa
Lucerne
Córdoba, Argentina
Bernanrdi Lima, Nelson
Conforto, Erica Cinthia
Serri, Dannae Lilia
Campilongo Mancilla, Ernesto Javier
Stenglein, Sebastian
Vargas Gil, Silvina
Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina
description Alfalfa is an important forage crop in Argentina, with a large area dedicated to its cultivation. However, plant diseases are a common cause of loss because they limit productivity and reduce forage quality. Anthracnose, caused by the Colletotrichum species, is one of the most important diseases affecting alfalfa worldwide. In July 2018, alfalfa plants with anthracnose symptoms were collected in commercial fields of Cordoba province, Argentina. Initially, infected plants showed small, pale gray lesions on stems, which turned oval and developed irregular dark brown/black borders as they enlarged. Three fungal isolates were obtained from the necrotic tissues of stems and single-spore purified. Based on the morphology and multilocus phylogenetic analysis, combined sequences of the partial glyceraldehyde-3-phosphate dehydrogenase (GAPDH), histone H3 (HIS3) and actin (ACT) genes, the three isolates were identified as Colletotrichum americae-borealis. Pathogenicity testing showed these isolates were pathogenic to alfalfa and Koch's postulates were verified by re-isolating the pathogen.
format info:ar-repo/semantics/artículo
topic_facet Medicago Sativa
Colletotrichum
Argentina
Anthracnosis
Phylogeny
Filogenia
Antracnosis
Molecular Phylogeny
Plant Pathogen
Alfalfa
Lucerne
Córdoba, Argentina
author Bernanrdi Lima, Nelson
Conforto, Erica Cinthia
Serri, Dannae Lilia
Campilongo Mancilla, Ernesto Javier
Stenglein, Sebastian
Vargas Gil, Silvina
author_facet Bernanrdi Lima, Nelson
Conforto, Erica Cinthia
Serri, Dannae Lilia
Campilongo Mancilla, Ernesto Javier
Stenglein, Sebastian
Vargas Gil, Silvina
author_sort Bernanrdi Lima, Nelson
title Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina
title_short Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina
title_full Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina
title_fullStr Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina
title_full_unstemmed Anthracnose on alfalfa caused by Colletotrichum americae-borealis in Córdoba province, Argentina
title_sort anthracnose on alfalfa caused by colletotrichum americae-borealis in córdoba province, argentina
publisher Elsevier
publishDate 2021-06-06
url http://hdl.handle.net/20.500.12123/9785
https://www.sciencedirect.com/science/article/pii/S0261219421002027
https://doi.org/10.1016/j.cropro.2021.105732
work_keys_str_mv AT bernanrdilimanelson anthracnoseonalfalfacausedbycolletotrichumamericaeborealisincordobaprovinceargentina
AT confortoericacinthia anthracnoseonalfalfacausedbycolletotrichumamericaeborealisincordobaprovinceargentina
AT serridannaelilia anthracnoseonalfalfacausedbycolletotrichumamericaeborealisincordobaprovinceargentina
AT campilongomancillaernestojavier anthracnoseonalfalfacausedbycolletotrichumamericaeborealisincordobaprovinceargentina
AT stengleinsebastian anthracnoseonalfalfacausedbycolletotrichumamericaeborealisincordobaprovinceargentina
AT vargasgilsilvina anthracnoseonalfalfacausedbycolletotrichumamericaeborealisincordobaprovinceargentina
_version_ 1756008087926341633