Comparative analysis of CRISPR/Cas9 delivery approaches for polyphenol oxidase 2 gene editing in potato
Poster
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | info:ar-repo/semantics/documento de conferencia biblioteca |
| Language: | eng |
| Published: |
Keystone Symposia on Molecular and Cellular Biology
2021-03
|
| Subjects: | Papa, Edición de Genes, Protoplastos, Agrobacterium Tumefaciens, Catecol Oxidasa, Potatoes, Gene Editing, Protoplasts, Catechol Oxidase, |
| Online Access: | http://hdl.handle.net/20.500.12123/9298 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
oai:localhost:20.500.12123-9298 |
|---|---|
| record_format |
koha |
| spelling |
oai:localhost:20.500.12123-92982021-05-06T18:08:57Z Comparative analysis of CRISPR/Cas9 delivery approaches for polyphenol oxidase 2 gene editing in potato Gonzalez, Matías Nicolás Massa, Gabriela Alejandra Anderson, Mariette Decima Oneto, Cecilia Andrea Turesson, Helle Storani, Leonardo Olsson, Niklas Fält, Ann Sofie Hofvander, Per Feingold, Sergio Enrique Papa Edición de Genes Protoplastos Agrobacterium Tumefaciens Catecol Oxidasa Potatoes Gene Editing Protoplasts Catechol Oxidase Poster StPPO2 gene editing was analyzed by Agrobacterium mediated transformation with CR-PPO vector, ribonucleoprotein complexes (RNP-PPO) transfection to protoplasts, and CR-PPO transient expression in protoplasts, yielding efficiencies of 9.6%, 18.4%, and 31.9%, respectively. Transient expression of CR-PPO in protoplasts resulted in tetra-allelic edited lines, observed in 46% of total edited lines. On-target DNA insertions were found in lines from all three approaches. Loss of function of the StPPO2 protein was confirmed in a tetra-allelic edited line. EEA Balcarce Fil: González, Matías Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Massa, Gabriela Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Massa, Gabriela Alejandra. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Fil: Anderson, Mariette. Swedish University of Agricultural Sciences. Department of Plant Breeding; Suecia. Fil: Décima Oneto, Cecilia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Décima Oneto, Cecilia Andrea. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Fil: Turesson, Helle. Swedish University of Agricultural Sciences. Department of Plant Breeding; Suecia. Fil: Storani, Leonardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Olsson, Niklas. Swedish University of Agricultural Sciences. Department of Plant Breeding; Suecia. Fil: Fält , Ann Sofie. Swedish University of Agricultural Sciences. Department of Plant Breeding; Suecia. Fil: Hofvander, Per. Swedish University of Agricultural Sciences. Department of Plant Breeding; Suecia. Fil: Feingold, Sergio Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. 2021-05-06T17:57:13Z 2021-05-06T17:57:13Z 2021-03 info:ar-repo/semantics/documento de conferencia info:eu-repo/semantics/conferenceObject info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9298 eng info:eu-repo/semantics/openAccess application/pdf Keystone Symposia on Molecular and Cellular Biology Keystone Symposia eSymposia meeting Plant Genome Engineering: From Lab to Field, Estados Unidos, 8-9 marzo de 2021 |
| institution |
INTA AR |
| collection |
DSpace |
| country |
Argentina |
| countrycode |
AR |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-inta-ar |
| tag |
biblioteca |
| region |
America del Sur |
| libraryname |
Biblioteca Central del INTA Argentina |
| language |
eng |
| topic |
Papa Edición de Genes Protoplastos Agrobacterium Tumefaciens Catecol Oxidasa Potatoes Gene Editing Protoplasts Catechol Oxidase Papa Edición de Genes Protoplastos Agrobacterium Tumefaciens Catecol Oxidasa Potatoes Gene Editing Protoplasts Catechol Oxidase |
| spellingShingle |
Papa Edición de Genes Protoplastos Agrobacterium Tumefaciens Catecol Oxidasa Potatoes Gene Editing Protoplasts Catechol Oxidase Papa Edición de Genes Protoplastos Agrobacterium Tumefaciens Catecol Oxidasa Potatoes Gene Editing Protoplasts Catechol Oxidase Gonzalez, Matías Nicolás Massa, Gabriela Alejandra Anderson, Mariette Decima Oneto, Cecilia Andrea Turesson, Helle Storani, Leonardo Olsson, Niklas Fält, Ann Sofie Hofvander, Per Feingold, Sergio Enrique Comparative analysis of CRISPR/Cas9 delivery approaches for polyphenol oxidase 2 gene editing in potato |
| description |
Poster |
| format |
info:ar-repo/semantics/documento de conferencia |
| topic_facet |
Papa Edición de Genes Protoplastos Agrobacterium Tumefaciens Catecol Oxidasa Potatoes Gene Editing Protoplasts Catechol Oxidase |
| author |
Gonzalez, Matías Nicolás Massa, Gabriela Alejandra Anderson, Mariette Decima Oneto, Cecilia Andrea Turesson, Helle Storani, Leonardo Olsson, Niklas Fält, Ann Sofie Hofvander, Per Feingold, Sergio Enrique |
| author_facet |
Gonzalez, Matías Nicolás Massa, Gabriela Alejandra Anderson, Mariette Decima Oneto, Cecilia Andrea Turesson, Helle Storani, Leonardo Olsson, Niklas Fält, Ann Sofie Hofvander, Per Feingold, Sergio Enrique |
| author_sort |
Gonzalez, Matías Nicolás |
| title |
Comparative analysis of CRISPR/Cas9 delivery approaches for polyphenol oxidase 2 gene editing in potato |
| title_short |
Comparative analysis of CRISPR/Cas9 delivery approaches for polyphenol oxidase 2 gene editing in potato |
| title_full |
Comparative analysis of CRISPR/Cas9 delivery approaches for polyphenol oxidase 2 gene editing in potato |
| title_fullStr |
Comparative analysis of CRISPR/Cas9 delivery approaches for polyphenol oxidase 2 gene editing in potato |
| title_full_unstemmed |
Comparative analysis of CRISPR/Cas9 delivery approaches for polyphenol oxidase 2 gene editing in potato |
| title_sort |
comparative analysis of crispr/cas9 delivery approaches for polyphenol oxidase 2 gene editing in potato |
| publisher |
Keystone Symposia on Molecular and Cellular Biology |
| publishDate |
2021-03 |
| url |
http://hdl.handle.net/20.500.12123/9298 |
| work_keys_str_mv |
AT gonzalezmatiasnicolas comparativeanalysisofcrisprcas9deliveryapproachesforpolyphenoloxidase2geneeditinginpotato AT massagabrielaalejandra comparativeanalysisofcrisprcas9deliveryapproachesforpolyphenoloxidase2geneeditinginpotato AT andersonmariette comparativeanalysisofcrisprcas9deliveryapproachesforpolyphenoloxidase2geneeditinginpotato AT decimaonetoceciliaandrea comparativeanalysisofcrisprcas9deliveryapproachesforpolyphenoloxidase2geneeditinginpotato AT turessonhelle comparativeanalysisofcrisprcas9deliveryapproachesforpolyphenoloxidase2geneeditinginpotato AT storanileonardo comparativeanalysisofcrisprcas9deliveryapproachesforpolyphenoloxidase2geneeditinginpotato AT olssonniklas comparativeanalysisofcrisprcas9deliveryapproachesforpolyphenoloxidase2geneeditinginpotato AT faltannsofie comparativeanalysisofcrisprcas9deliveryapproachesforpolyphenoloxidase2geneeditinginpotato AT hofvanderper comparativeanalysisofcrisprcas9deliveryapproachesforpolyphenoloxidase2geneeditinginpotato AT feingoldsergioenrique comparativeanalysisofcrisprcas9deliveryapproachesforpolyphenoloxidase2geneeditinginpotato |
| _version_ |
1756008024177115136 |