Molecular characterization of Brucella ovis in Argentina
Brucellosis in rams is caused by Brucella ovis or Brucella melitensis and it is considered one of the most important infectious diseases of males in sheep-raising countries. Molecular characterization of Brucella spp. achieved by multi-locus variable number of tandem repeats analysis (MLVA) is a powerful tool to genotype Brucella spp. However, data regarding B. ovis genotyping is scarce. Thus, the aim of this study was to characterize the molecular diversity of B. ovis field-strains in Argentina. A total of 115 isolates of B. ovis from Argentina and Uruguay were genotyped using MLVA-16 and analyzed altogether with 14 publicly available B. ovis genotypes from Brazil. The Discriminatory Power (D) was 0.996 for MLVA-16 and 0.0998 for MLVA-8 and MLVA-11. Analysis of MLVA-16 revealed 100 different genotypes, all of them novel, including 90 unique ones. There was no correlation between geographical distribution and genotype and results showed a higher diversity within provinces than between provinces. Clustering analysis of the strains from Argentina, Uruguay and Brazil revealed that the 129 isolates were grouped into two clades. Whole Genome Sequencing analysis of the 19 B. ovis genomes available in public databases, and including some of the Argentinian strains used in this study, revealed clustering of the Argentinian isolates and closer relationship with B. ovis from New Zealand and Australia. This work adds new data to the poorly understood distribution map of genotypes regionally and worldwide for B. ovis and it constitutes the largest study of B. ovis molecular genotyping until now.
Main Authors: | , , , , , , , |
---|---|
Format: | info:ar-repo/semantics/artículo biblioteca |
Language: | eng |
Published: |
Elsevier
2020-06
|
Subjects: | Brucella Ovis, Brucella, Brucelosis, Brucella Melitensis, Genotipos, Enfermedades de los Animales, Brucellosis, Genotypes, Animal Diseases, Argentina, |
Online Access: | http://hdl.handle.net/20.500.12123/7928 https://www.sciencedirect.com/science/article/pii/S037811351931452X https://doi.org/10.1016/j.vetmic.2020.108703 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:localhost:20.500.12123-7928 |
---|---|
record_format |
koha |
spelling |
oai:localhost:20.500.12123-79282020-09-21T12:25:30Z Molecular characterization of Brucella ovis in Argentina Alvarez, Lucía Paula Ruiz-Villalobos, Nazaret Suárez-Esquivel, Marcela Thomson, Nicholas R. Marcellino, Romanela Víquez-Ruiz, Eunice Robles, Carlos Alejandro Guzmán-Verri, Caterina Brucella Ovis Brucella Brucelosis Brucella Melitensis Genotipos Enfermedades de los Animales Brucellosis Genotypes Animal Diseases Argentina Brucellosis in rams is caused by Brucella ovis or Brucella melitensis and it is considered one of the most important infectious diseases of males in sheep-raising countries. Molecular characterization of Brucella spp. achieved by multi-locus variable number of tandem repeats analysis (MLVA) is a powerful tool to genotype Brucella spp. However, data regarding B. ovis genotyping is scarce. Thus, the aim of this study was to characterize the molecular diversity of B. ovis field-strains in Argentina. A total of 115 isolates of B. ovis from Argentina and Uruguay were genotyped using MLVA-16 and analyzed altogether with 14 publicly available B. ovis genotypes from Brazil. The Discriminatory Power (D) was 0.996 for MLVA-16 and 0.0998 for MLVA-8 and MLVA-11. Analysis of MLVA-16 revealed 100 different genotypes, all of them novel, including 90 unique ones. There was no correlation between geographical distribution and genotype and results showed a higher diversity within provinces than between provinces. Clustering analysis of the strains from Argentina, Uruguay and Brazil revealed that the 129 isolates were grouped into two clades. Whole Genome Sequencing analysis of the 19 B. ovis genomes available in public databases, and including some of the Argentinian strains used in this study, revealed clustering of the Argentinian isolates and closer relationship with B. ovis from New Zealand and Australia. This work adds new data to the poorly understood distribution map of genotypes regionally and worldwide for B. ovis and it constitutes the largest study of B. ovis molecular genotyping until now. Estación Experimental Agropecuaria Bariloche Fil: Alvarez, Lucía Paula. Universidad Nacional del Comahue. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina Fil: Ruiz-Villalobos, Nazaret. Universidad Nacional. Escuela de Medicina Veterinaria; Costa Rica Fil: Suárez-Esquivel, Marcela. Universidad Nacional. Escuela de Medicina Veterinaria; Costa Rica Fil: Thomson, Nicholas R. Wellcome Trust Sanger Institute. Parasites and Microbes from Pathogen Genomics; Reino Unido Fil: Marcellino, Romanela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Producción Animal. Grupo de Sanidad Animal; Argentina Fil: Víquez-Ruiz, Eunice. Universidad Nacional. Escuela de Medicina Veterinaria; Costa Rica Fil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area de Produccion Animal. Grupo de Sanidad Animal; Argentina Fil: Guzmán-Verri, Caterina. Universidad Nacional. Escuela de Medicina Veterinaria; Costa Rica 2020-09-21T12:17:40Z 2020-09-21T12:17:40Z 2020-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7928 https://www.sciencedirect.com/science/article/pii/S037811351931452X 0378-1135 https://doi.org/10.1016/j.vetmic.2020.108703 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary Microbiology 245 : Art. 108703 (June 2020) |
institution |
INTA AR |
collection |
DSpace |
country |
Argentina |
countrycode |
AR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-inta-ar |
tag |
biblioteca |
region |
America del Sur |
libraryname |
Biblioteca Central del INTA Argentina |
language |
eng |
topic |
Brucella Ovis Brucella Brucelosis Brucella Melitensis Genotipos Enfermedades de los Animales Brucellosis Genotypes Animal Diseases Argentina Brucella Ovis Brucella Brucelosis Brucella Melitensis Genotipos Enfermedades de los Animales Brucellosis Genotypes Animal Diseases Argentina |
spellingShingle |
Brucella Ovis Brucella Brucelosis Brucella Melitensis Genotipos Enfermedades de los Animales Brucellosis Genotypes Animal Diseases Argentina Brucella Ovis Brucella Brucelosis Brucella Melitensis Genotipos Enfermedades de los Animales Brucellosis Genotypes Animal Diseases Argentina Alvarez, Lucía Paula Ruiz-Villalobos, Nazaret Suárez-Esquivel, Marcela Thomson, Nicholas R. Marcellino, Romanela Víquez-Ruiz, Eunice Robles, Carlos Alejandro Guzmán-Verri, Caterina Molecular characterization of Brucella ovis in Argentina |
description |
Brucellosis in rams is caused by Brucella ovis or Brucella melitensis and it is considered one of the most important infectious diseases of males in sheep-raising countries. Molecular characterization of Brucella spp. achieved by multi-locus variable number of tandem repeats analysis (MLVA) is a powerful tool to genotype Brucella spp. However, data regarding B. ovis genotyping is scarce. Thus, the aim of this study was to characterize the molecular diversity of B. ovis field-strains in Argentina. A total of 115 isolates of B. ovis from Argentina and Uruguay were genotyped using MLVA-16 and analyzed altogether with 14 publicly available B. ovis genotypes from
Brazil. The Discriminatory Power (D) was 0.996 for MLVA-16 and 0.0998 for MLVA-8 and MLVA-11. Analysis of MLVA-16 revealed 100 different genotypes, all of them novel, including 90 unique ones. There was no correlation between geographical distribution and genotype and results showed a higher diversity within provinces than between provinces. Clustering analysis of the strains from Argentina, Uruguay and Brazil revealed that the 129 isolates were grouped into two clades. Whole Genome Sequencing analysis of the 19 B. ovis genomes available in public databases, and including some of the Argentinian strains used in this study, revealed clustering
of the Argentinian isolates and closer relationship with B. ovis from New Zealand and Australia. This work adds new data to the poorly understood distribution map of genotypes regionally and worldwide for B. ovis and it constitutes the largest study of B. ovis molecular genotyping until now. |
format |
info:ar-repo/semantics/artículo |
topic_facet |
Brucella Ovis Brucella Brucelosis Brucella Melitensis Genotipos Enfermedades de los Animales Brucellosis Genotypes Animal Diseases Argentina |
author |
Alvarez, Lucía Paula Ruiz-Villalobos, Nazaret Suárez-Esquivel, Marcela Thomson, Nicholas R. Marcellino, Romanela Víquez-Ruiz, Eunice Robles, Carlos Alejandro Guzmán-Verri, Caterina |
author_facet |
Alvarez, Lucía Paula Ruiz-Villalobos, Nazaret Suárez-Esquivel, Marcela Thomson, Nicholas R. Marcellino, Romanela Víquez-Ruiz, Eunice Robles, Carlos Alejandro Guzmán-Verri, Caterina |
author_sort |
Alvarez, Lucía Paula |
title |
Molecular characterization of Brucella ovis in Argentina |
title_short |
Molecular characterization of Brucella ovis in Argentina |
title_full |
Molecular characterization of Brucella ovis in Argentina |
title_fullStr |
Molecular characterization of Brucella ovis in Argentina |
title_full_unstemmed |
Molecular characterization of Brucella ovis in Argentina |
title_sort |
molecular characterization of brucella ovis in argentina |
publisher |
Elsevier |
publishDate |
2020-06 |
url |
http://hdl.handle.net/20.500.12123/7928 https://www.sciencedirect.com/science/article/pii/S037811351931452X https://doi.org/10.1016/j.vetmic.2020.108703 |
work_keys_str_mv |
AT alvarezluciapaula molecularcharacterizationofbrucellaovisinargentina AT ruizvillalobosnazaret molecularcharacterizationofbrucellaovisinargentina AT suarezesquivelmarcela molecularcharacterizationofbrucellaovisinargentina AT thomsonnicholasr molecularcharacterizationofbrucellaovisinargentina AT marcellinoromanela molecularcharacterizationofbrucellaovisinargentina AT viquezruizeunice molecularcharacterizationofbrucellaovisinargentina AT roblescarlosalejandro molecularcharacterizationofbrucellaovisinargentina AT guzmanverricaterina molecularcharacterizationofbrucellaovisinargentina |
_version_ |
1756007843764371456 |