The Effect of X-Rays on Cytological Traits of Tuta absoluta (Lepidoptera: Gelechiidae)
The tomato leafminer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) is one of the most important pests of tomato. With the purpose of developing environmentally friendly control tactics such as the inherited sterility (IS) technique against this species, it is essential to understand its genetics and biology. In this paper we analyzed the karyotype, sperm morphology and sperm ratio in wild-type and X-ray irradiated individuals of T. absoluta. The diploid chromosome number of T. absoluta was 2n = 58 including the pair of sex chromosomes: ZZ in males and WZ in females, which were the largest elements of the complement. Irradiation of pupae in an X-ray machine with a dose of 200 Gy generated various types of chromosomal rearrangements including translocations and fragmentations, resulting in altered chromosome numbers. The analysis of spermatozoa in T. absoluta revealed a significant morphological difference between apyrene and eupyrene sperm bundles. Irradiation with X-ray doses of 100, 150, 200 and 250 Gy did not have a significant effect on the apyrene to eupyrene sperm ratio. However, males irradiated with 300 Gy produced significantly more apyrene sperm than non-irradiated males. All the doses applied influenced the morphology of eupyrene sperm bundles. The modified eupyrene sperm bundles could be used as a bioindicator during the monitoring of an IS program after the release of irradiated males. We found that the modified eupyrene spermatozoa were transferred to the bursae copulatrices of the females. Males treated with 200 Gy transferred a greater proportion of modified eupyrene sperm than untreated males. The results presented herein provide essential information on the cytology of T. absoluta, which is required to evaluate the quality of the released insects, and for better understanding and application of IS against this economically important pest.
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Format: | info:eu-repo/semantics/article biblioteca |
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2016
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Subjects: | Plagas de Plantas, Scrobipalpula Absoluta, Rayos-x, Pest of Plants, X Rays, Tuta Absoluta, Polilla del Tomate, |
Online Access: | http://hdl.handle.net/20.500.12123/787 http://www.bioone.org/doi/pdf/10.1653/024.099.sp107 https://doi.org/10.1653/024.099.sp107 |
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Plagas de Plantas Scrobipalpula Absoluta Rayos-x Pest of Plants X Rays Tuta Absoluta Polilla del Tomate Plagas de Plantas Scrobipalpula Absoluta Rayos-x Pest of Plants X Rays Tuta Absoluta Polilla del Tomate |
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Plagas de Plantas Scrobipalpula Absoluta Rayos-x Pest of Plants X Rays Tuta Absoluta Polilla del Tomate Plagas de Plantas Scrobipalpula Absoluta Rayos-x Pest of Plants X Rays Tuta Absoluta Polilla del Tomate Carabajal Paladino, Leonale Suzel Ferrari, María E. Lauría, Juan P. Cagnotti, Cynthia Lorena Šíchová, Jindra López, Silvia Noemi The Effect of X-Rays on Cytological Traits of Tuta absoluta (Lepidoptera: Gelechiidae) |
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The tomato leafminer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) is one of the most important pests of tomato. With the purpose of developing environmentally friendly control tactics such as the inherited sterility (IS) technique against this species, it is essential to understand its genetics and biology. In this paper we analyzed the karyotype, sperm morphology and sperm ratio in wild-type and X-ray irradiated individuals of T. absoluta. The diploid chromosome number of T. absoluta was 2n = 58 including the pair of sex chromosomes: ZZ in males and WZ in females, which were the largest elements of the complement. Irradiation of pupae in an X-ray machine with a dose of 200 Gy generated various types of chromosomal rearrangements including translocations and fragmentations, resulting in altered chromosome numbers. The analysis of spermatozoa in T. absoluta revealed a significant morphological difference between apyrene and eupyrene sperm bundles. Irradiation with X-ray doses of 100, 150, 200 and 250 Gy did not have a significant effect on the apyrene to eupyrene sperm ratio. However, males irradiated with 300 Gy produced significantly more apyrene sperm than non-irradiated males. All the doses applied influenced the morphology of eupyrene sperm bundles. The modified eupyrene sperm bundles could be used as a bioindicator during the monitoring of an IS program after the release of irradiated males. We found that the modified eupyrene spermatozoa were transferred to the bursae copulatrices of the females. Males treated with 200 Gy transferred a greater proportion of modified eupyrene sperm than untreated males. The results presented herein provide essential information on the cytology of T. absoluta, which is required to evaluate the quality of the released insects, and for better understanding and application of IS against this economically important pest. |
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Plagas de Plantas Scrobipalpula Absoluta Rayos-x Pest of Plants X Rays Tuta Absoluta Polilla del Tomate |
author |
Carabajal Paladino, Leonale Suzel Ferrari, María E. Lauría, Juan P. Cagnotti, Cynthia Lorena Šíchová, Jindra López, Silvia Noemi |
author_facet |
Carabajal Paladino, Leonale Suzel Ferrari, María E. Lauría, Juan P. Cagnotti, Cynthia Lorena Šíchová, Jindra López, Silvia Noemi |
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Carabajal Paladino, Leonale Suzel |
title |
The Effect of X-Rays on Cytological Traits of Tuta absoluta (Lepidoptera: Gelechiidae) |
title_short |
The Effect of X-Rays on Cytological Traits of Tuta absoluta (Lepidoptera: Gelechiidae) |
title_full |
The Effect of X-Rays on Cytological Traits of Tuta absoluta (Lepidoptera: Gelechiidae) |
title_fullStr |
The Effect of X-Rays on Cytological Traits of Tuta absoluta (Lepidoptera: Gelechiidae) |
title_full_unstemmed |
The Effect of X-Rays on Cytological Traits of Tuta absoluta (Lepidoptera: Gelechiidae) |
title_sort |
effect of x-rays on cytological traits of tuta absoluta (lepidoptera: gelechiidae) |
publishDate |
2016 |
url |
http://hdl.handle.net/20.500.12123/787 http://www.bioone.org/doi/pdf/10.1653/024.099.sp107 https://doi.org/10.1653/024.099.sp107 |
work_keys_str_mv |
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oai:localhost:20.500.12123-7872018-01-12T14:24:05Z The Effect of X-Rays on Cytological Traits of Tuta absoluta (Lepidoptera: Gelechiidae) Carabajal Paladino, Leonale Suzel Ferrari, María E. Lauría, Juan P. Cagnotti, Cynthia Lorena Šíchová, Jindra López, Silvia Noemi Plagas de Plantas Scrobipalpula Absoluta Rayos-x Pest of Plants X Rays Tuta Absoluta Polilla del Tomate The tomato leafminer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) is one of the most important pests of tomato. With the purpose of developing environmentally friendly control tactics such as the inherited sterility (IS) technique against this species, it is essential to understand its genetics and biology. In this paper we analyzed the karyotype, sperm morphology and sperm ratio in wild-type and X-ray irradiated individuals of T. absoluta. The diploid chromosome number of T. absoluta was 2n = 58 including the pair of sex chromosomes: ZZ in males and WZ in females, which were the largest elements of the complement. Irradiation of pupae in an X-ray machine with a dose of 200 Gy generated various types of chromosomal rearrangements including translocations and fragmentations, resulting in altered chromosome numbers. The analysis of spermatozoa in T. absoluta revealed a significant morphological difference between apyrene and eupyrene sperm bundles. Irradiation with X-ray doses of 100, 150, 200 and 250 Gy did not have a significant effect on the apyrene to eupyrene sperm ratio. However, males irradiated with 300 Gy produced significantly more apyrene sperm than non-irradiated males. All the doses applied influenced the morphology of eupyrene sperm bundles. The modified eupyrene sperm bundles could be used as a bioindicator during the monitoring of an IS program after the release of irradiated males. We found that the modified eupyrene spermatozoa were transferred to the bursae copulatrices of the females. Males treated with 200 Gy transferred a greater proportion of modified eupyrene sperm than untreated males. The results presented herein provide essential information on the cytology of T. absoluta, which is required to evaluate the quality of the released insects, and for better understanding and application of IS against this economically important pest. La polilla del tomate Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) es una de las plagas más importantes del cultivo de tomate. Con el objeto de desarrollar estrategias para su control que sean respetuosas con el ambiente, como por ejemplo la técnica de esterilidad heredada (EH), es indispensable entender su genética y su biología. En este trabajo analizamos el cariotipo, la morfología espermática y la proporción de esperma en individuos salvajes e irradiados con rayos X. El número cromosómico diploide de T. absoluta es 2n = 58, incluyendo el par de cromosomas sexuales: ZZ en machos y WZ en hembras, que constituyen los elementos de mayor tamaño en el complemento. La irradiación de pupas en un irradiador de rayos X con una dosis de 200 Gy generó diferentes reordenamientos cromosómicos incluyendo translocaciones y fragmentaciones, resultando en alteraciones en el número cromosómico. El análisis de los paquetes de espermatozoides de T. absoluta reveló un marcado polimorfismo entre los paquetes de esperma apireno y eupireno. La irradiación de los individuos con dosis de 100, 150, 200 y 250 Gy no tuvo un efecto significativo sobre la proporción de esperma apireno:eupireno. Sin embargo, los machos irradiados con 300 Gy produjeron una cantidad significativamente mayor de espermatozoides apirenos que los no irradiados. Todas las dosis de radiación influenciaron la morfología de los paquetes de esperma eupireno. Estos paquetes deformes podrían ser utilizados como bioindicadores durante el proceso de monitoreo llevado a cabo en los programas de EH luego de la liberación de machos irradiados. Los espermatozoides eupirenos deformes fueron transferidos a las bursae copulatrices de las hembras durante la cópula. Los machos tratados con 200 Gy transfirieron una cantidad mayor de espermatozoides deformes que los individuos no irradiados. Los resultados aquí presentados proveen información esencial sobre la citología de T. absoluta, la cual es necesaria para evaluar la calidad de los insectos liberados y para un mejor entendimiento y aplicación de EH contra esta especie de importancia económica mundial. Inst. de Microbiología y Zoología Agrícola IMyZA Fil: Carabajal Paladino, Leonale Suzel. Biology Centre CAS. Institute of Entomology; República Checa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Ferrari, María E. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Lauría, Juan P. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Cagnotti, Cynthia Lorena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Šíchová, Jindra. Biology Centre CAS. Institute of Entomology; República Checa. University of South Bohemia. Faculty of Science; República Checa Fil: López, Silvia Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina 2017-07-26T16:51:12Z 2017-07-26T16:51:12Z 2016 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/787 http://www.bioone.org/doi/pdf/10.1653/024.099.sp107 1938-5102 https://doi.org/10.1653/024.099.sp107 eng info:eu-repo/semantics/openAccess application/pdf Florida Entomologist 99 (sp1): 43-53 |