Detection methods and characterization of bovine viral diarrhea virus in aborted fetuses and neonatal calves over a 22-year period
Detection of bovine viral diarrhea virus (BVDV) in aborted fetus samples is often difficult due to tissue autolysis and inappropriate sampling. Studies assessing different methods for BVDV identification in fetal specimens are scarce. The present study evaluated the agreement between different diagnostic techniques to detect BVDV infections in specimens from a large number of bovine aborted fetuses and neonatal deaths over a period of 22 years. Additionally, genetic, serological, and pathological analyses were conducted in order to characterize BVDV strains of fetal origin. Samples from 95 selected cases from 1997 to 2018 were analyzed by antigen-capture ELISA (AgELISA), nested RT-PCR (RT-nPCR), and real-time RT-PCR (RT-qPCR). In addition, amplification and sequencing of the 5'UTR region were performed for phylogenetic purposes. Virus neutralization tests against the BVDV-1a, BVDV-1b, and BVDV-2b subtypes were conducted on 60 fetal fluids of the selected cases. Furthermore, the frequency and severity of histopathological lesions were evaluated in BVDV-positive cases. This study demonstrated that RT-nPCR and RT-qPCR were more suitable than AgELISA for BVDV detection in fetal specimens. However, the agreement between the two RT-PCR methods was moderate. The BVDV-1b subtype was more frequently detected than the BVDV-1a and BVDV-2b subtypes. Neutralizing antibodies to any of the three subtypes evaluated were present in 94% of the fetal fluids. Microscopically, half of the BVDV-positive cases showed a mild non-suppurative inflammatory response. These results emphasize the need to consider different methods for a diagnostic approach of BVDV associated to reproductive losses.
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2020-05-16
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Subjects: | Aborto, Diagnóstico, Filogenia, Patología, Abortion, Diagnosis, Phylogeny, Pathology, BVDV, |
Online Access: | http://hdl.handle.net/20.500.12123/7354 https://link.springer.com/article/10.1007/s42770-020-00296-z https://doi.org/10.1007/s42770-020-00296-z |
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Aborto Diagnóstico Filogenia Patología Abortion Diagnosis Phylogeny Pathology BVDV Aborto Diagnóstico Filogenia Patología Abortion Diagnosis Phylogeny Pathology BVDV |
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Aborto Diagnóstico Filogenia Patología Abortion Diagnosis Phylogeny Pathology BVDV Aborto Diagnóstico Filogenia Patología Abortion Diagnosis Phylogeny Pathology BVDV Spetter, Lucas Maximiliano Louge Uriarte, Enrique Leopoldo Armendano, Joaquín Ignacio Morrell, Eleonora Lidia Canton, German Jose Verna, Andrea Elizabeth Dorsch, Matías Pereyra, Susana Beatriz Odeon, Anselmo Carlos Saliki, Jeremiah González Altamiranda, Erika Detection methods and characterization of bovine viral diarrhea virus in aborted fetuses and neonatal calves over a 22-year period |
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Detection of bovine viral diarrhea virus (BVDV) in aborted fetus samples is often difficult due to tissue autolysis and inappropriate sampling. Studies assessing different methods for BVDV identification in fetal specimens are scarce. The present study evaluated the agreement between different diagnostic techniques to detect BVDV infections in specimens from a large number of bovine aborted fetuses and neonatal deaths over a period of 22 years. Additionally, genetic, serological, and pathological analyses were conducted in order to characterize BVDV strains of fetal origin. Samples from 95 selected cases from 1997 to 2018 were analyzed by antigen-capture ELISA (AgELISA), nested RT-PCR (RT-nPCR), and real-time RT-PCR (RT-qPCR). In addition, amplification and sequencing of the 5'UTR region were performed for phylogenetic purposes. Virus neutralization tests against the BVDV-1a, BVDV-1b, and BVDV-2b subtypes were conducted on 60 fetal fluids of the selected cases. Furthermore, the frequency and severity of histopathological lesions were evaluated in BVDV-positive cases. This study demonstrated that RT-nPCR and RT-qPCR were more suitable than AgELISA for BVDV detection in fetal specimens. However, the agreement between the two RT-PCR methods was moderate. The BVDV-1b subtype was more frequently detected than the BVDV-1a and BVDV-2b subtypes. Neutralizing antibodies to any of the three subtypes evaluated were present in 94% of the fetal fluids. Microscopically, half of the BVDV-positive cases showed a mild non-suppurative inflammatory response. These results emphasize the need to consider different methods for a diagnostic approach of BVDV associated to reproductive losses. |
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Aborto Diagnóstico Filogenia Patología Abortion Diagnosis Phylogeny Pathology BVDV |
author |
Spetter, Lucas Maximiliano Louge Uriarte, Enrique Leopoldo Armendano, Joaquín Ignacio Morrell, Eleonora Lidia Canton, German Jose Verna, Andrea Elizabeth Dorsch, Matías Pereyra, Susana Beatriz Odeon, Anselmo Carlos Saliki, Jeremiah González Altamiranda, Erika |
author_facet |
Spetter, Lucas Maximiliano Louge Uriarte, Enrique Leopoldo Armendano, Joaquín Ignacio Morrell, Eleonora Lidia Canton, German Jose Verna, Andrea Elizabeth Dorsch, Matías Pereyra, Susana Beatriz Odeon, Anselmo Carlos Saliki, Jeremiah González Altamiranda, Erika |
author_sort |
Spetter, Lucas Maximiliano |
title |
Detection methods and characterization of bovine viral diarrhea virus in aborted fetuses and neonatal calves over a 22-year period |
title_short |
Detection methods and characterization of bovine viral diarrhea virus in aborted fetuses and neonatal calves over a 22-year period |
title_full |
Detection methods and characterization of bovine viral diarrhea virus in aborted fetuses and neonatal calves over a 22-year period |
title_fullStr |
Detection methods and characterization of bovine viral diarrhea virus in aborted fetuses and neonatal calves over a 22-year period |
title_full_unstemmed |
Detection methods and characterization of bovine viral diarrhea virus in aborted fetuses and neonatal calves over a 22-year period |
title_sort |
detection methods and characterization of bovine viral diarrhea virus in aborted fetuses and neonatal calves over a 22-year period |
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Springer |
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2020-05-16 |
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http://hdl.handle.net/20.500.12123/7354 https://link.springer.com/article/10.1007/s42770-020-00296-z https://doi.org/10.1007/s42770-020-00296-z |
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oai:localhost:20.500.12123-73542020-06-03T18:24:23Z Detection methods and characterization of bovine viral diarrhea virus in aborted fetuses and neonatal calves over a 22-year period Spetter, Lucas Maximiliano Louge Uriarte, Enrique Leopoldo Armendano, Joaquín Ignacio Morrell, Eleonora Lidia Canton, German Jose Verna, Andrea Elizabeth Dorsch, Matías Pereyra, Susana Beatriz Odeon, Anselmo Carlos Saliki, Jeremiah González Altamiranda, Erika Aborto Diagnóstico Filogenia Patología Abortion Diagnosis Phylogeny Pathology BVDV Detection of bovine viral diarrhea virus (BVDV) in aborted fetus samples is often difficult due to tissue autolysis and inappropriate sampling. Studies assessing different methods for BVDV identification in fetal specimens are scarce. The present study evaluated the agreement between different diagnostic techniques to detect BVDV infections in specimens from a large number of bovine aborted fetuses and neonatal deaths over a period of 22 years. Additionally, genetic, serological, and pathological analyses were conducted in order to characterize BVDV strains of fetal origin. Samples from 95 selected cases from 1997 to 2018 were analyzed by antigen-capture ELISA (AgELISA), nested RT-PCR (RT-nPCR), and real-time RT-PCR (RT-qPCR). In addition, amplification and sequencing of the 5'UTR region were performed for phylogenetic purposes. Virus neutralization tests against the BVDV-1a, BVDV-1b, and BVDV-2b subtypes were conducted on 60 fetal fluids of the selected cases. Furthermore, the frequency and severity of histopathological lesions were evaluated in BVDV-positive cases. This study demonstrated that RT-nPCR and RT-qPCR were more suitable than AgELISA for BVDV detection in fetal specimens. However, the agreement between the two RT-PCR methods was moderate. The BVDV-1b subtype was more frequently detected than the BVDV-1a and BVDV-2b subtypes. Neutralizing antibodies to any of the three subtypes evaluated were present in 94% of the fetal fluids. Microscopically, half of the BVDV-positive cases showed a mild non-suppurative inflammatory response. These results emphasize the need to consider different methods for a diagnostic approach of BVDV associated to reproductive losses. EEA Balcarce Fil: Spetter, Maximiliano Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Armendano, Joaquín Ignacio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Verna, Andrea Elizabeth . Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. Fil: Dorsch, Matías A. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Pereyra, Susana Beatriz . Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Odeón, Anselmo C. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Saliki, Jeremiah T. University of Georgia. Athens Veterinary Diagnostic Laboratory; Estados Unidos Fil: González Altamiranda, Erika A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina 2020-06-03T17:41:33Z 2020-06-03T17:41:33Z 2020-05-16 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7354 https://link.springer.com/article/10.1007/s42770-020-00296-z 1517-8382 https://doi.org/10.1007/s42770-020-00296-z eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Brazilian journal of microbiology (2020) |