Matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae
In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future.
Main Authors: | Bacanelli, Gisele, Olarte, Larissa C., Silva, Marcio Roberto, Rodrigues, Rudielle A., Carneiro, Paulo A. M., Kannene, John B., Pasquatti, Taynara N., Takatani, Haruo, Zumarraga, Martin Jose, Etges, Rodrigo N., Araujo, Flabio Ribeiro de, Verbisck, Newton V. |
---|---|
Format: | info:ar-repo/semantics/artículo biblioteca |
Language: | eng |
Published: |
The Japanese Society of Veterinary Science
2019-10
|
Subjects: | Tuberculosis, Infeccion por Mycobacterium bovis, Tratamiento Ionizante, Desorción, Espectrometría, Infeccion Mycobacterium tuberculosis, Bovina, Mycobacterium bovis Infections, Ionization Treatment, Desorption, Spectrometry, Mycobacterium tuberculosis Infections, Bovinae, |
Online Access: | https://www.jstage.jst.go.jp/article/jvms/81/10/81_19-0214/_article http://hdl.handle.net/20.500.12123/6567 https://doi.org/10.1292/jvms.19-0214 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
The subunit vaccine H65 + CAF01 increased the BCG- protection against Mycobacterium bovis infection in a mouse model of bovine tuberculosis
by: Blanco, Federico Carlos, et al.
Published: (2021-05) -
Mycobacterium bovis ESAT-6, CFP-10 and EspC antigens show high conservation among field isolates
by: Encinas, Micaela, et al.
Published: (2018-07) -
Desarrollo de alternativas diagnósticas de tuberculosis bovina
by: Leiva, Carlos
Published: (2015-02) -
Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles
by: Bigi, María Mercedes, et al.
Published: (2019-12) -
Development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosis
by: Alonso, Maria Natalia, et al.
Published: (2021-04)