Antioxidant effect of lemon balm (Melissa officinalis) and mate tea (Ilex paraguensys) on quality, lipid peroxidation and DNA oxidation of cryopreserved boar epididymal spermatozoa
In this study, we investigated the protective ability of the addition of two antioxidant herb extracts, mate tea and lemon balm, on boar epididymal frozen–thawed spermatozoa quality. Testes from mature boars were collected at local slaughterhouse, and sperm samples from epididymis were recovered by flushing. Spermatozoa were cryopreserved in lactose–egg yolk buffer supplemented with various concentrations of lemon balm and mate tea (0, 2.5, 5 and 10 g l−1) using the straw‐freezing procedure. Motion parameters, acrosome and plasma membrane integrity, lipoperoxidation levels and DNA oxidative damage (8‐hydroxy‐2′‐deoxyguanosine base lesion) were evaluated. There were no differences among experimental groups with regard to motility characteristics, viability, acrosome and plasma membrane integrity; however, the highest concentration of lemon balm produced significant (P < 0.05) improvement in curvilinear trajectory, straightness and amplitude of lateral head displacement after thawing. The supplementation of freezing extender with mate tea and lemon balm reduced sperm lipid membrane peroxidation, and only mate tea protected DNA against oxidative damage during cryopreservation at 120 min post‐thawing (P < 0.05). Mate tea experimental extender at concentration of 10 g l−1 showed the lowest percentage of sperm oxidised DNA and malondialdehyde generation; thus, mate tea is a potential candidate such as antioxidant compound on boar sperm cryopreservation medium.
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Wiley
2015-11
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Subjects: | Ilex paraguariensis, Mate, Elissa officinalis, Antioxidantes, ADN, Espermatozoo, Criopreservación, Verraco, Cerdo, Antioxidants, DNA, Spermatozoa, Cryopreservation, Boars, Swine, Melissa officinalis, Esperma, |
Online Access: | https://onlinelibrary.wiley.com/doi/abs/10.1111/and.12370 http://hdl.handle.net/20.500.12123/4737 https://doi.org/10.1111/and.12370 |
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oai:localhost:20.500.12123-47372019-06-07T18:16:32Z Antioxidant effect of lemon balm (Melissa officinalis) and mate tea (Ilex paraguensys) on quality, lipid peroxidation and DNA oxidation of cryopreserved boar epididymal spermatozoa Luño, Victoria Gil, María Lydia Olaciregui, Maite Jerez, Rodrigo A. De Blas, José Ignacio Hozbor, Federico Andres Ilex paraguariensis Mate Elissa officinalis Antioxidantes ADN Espermatozoo Criopreservación Verraco Cerdo Antioxidants DNA Spermatozoa Cryopreservation Boars Swine Melissa officinalis Esperma In this study, we investigated the protective ability of the addition of two antioxidant herb extracts, mate tea and lemon balm, on boar epididymal frozen–thawed spermatozoa quality. Testes from mature boars were collected at local slaughterhouse, and sperm samples from epididymis were recovered by flushing. Spermatozoa were cryopreserved in lactose–egg yolk buffer supplemented with various concentrations of lemon balm and mate tea (0, 2.5, 5 and 10 g l−1) using the straw‐freezing procedure. Motion parameters, acrosome and plasma membrane integrity, lipoperoxidation levels and DNA oxidative damage (8‐hydroxy‐2′‐deoxyguanosine base lesion) were evaluated. There were no differences among experimental groups with regard to motility characteristics, viability, acrosome and plasma membrane integrity; however, the highest concentration of lemon balm produced significant (P < 0.05) improvement in curvilinear trajectory, straightness and amplitude of lateral head displacement after thawing. The supplementation of freezing extender with mate tea and lemon balm reduced sperm lipid membrane peroxidation, and only mate tea protected DNA against oxidative damage during cryopreservation at 120 min post‐thawing (P < 0.05). Mate tea experimental extender at concentration of 10 g l−1 showed the lowest percentage of sperm oxidised DNA and malondialdehyde generation; thus, mate tea is a potential candidate such as antioxidant compound on boar sperm cryopreservation medium. EEA Balcarce Fil: Luño, Victoria. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; España Fil: Gil, María Lydia. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; España Fil: Olaciregui, Maite. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; España Fil: Jerez, Rodrigo A. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; España Fil: de Blas, Ignacio. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; España Fil: Hozbor, Federico Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina 2019-03-26T12:11:57Z 2019-03-26T12:11:57Z 2015-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://onlinelibrary.wiley.com/doi/abs/10.1111/and.12370 http://hdl.handle.net/20.500.12123/4737 0303-4569 1439-0272 https://doi.org/10.1111/and.12370 eng info:eu-repo/semantics/restrictedAccess application/pdf Wiley Andrologia 47 (9) : 1004-1011 (November 2015) |
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Ilex paraguariensis Mate Elissa officinalis Antioxidantes ADN Espermatozoo Criopreservación Verraco Cerdo Antioxidants DNA Spermatozoa Cryopreservation Boars Swine Melissa officinalis Esperma Ilex paraguariensis Mate Elissa officinalis Antioxidantes ADN Espermatozoo Criopreservación Verraco Cerdo Antioxidants DNA Spermatozoa Cryopreservation Boars Swine Melissa officinalis Esperma |
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Ilex paraguariensis Mate Elissa officinalis Antioxidantes ADN Espermatozoo Criopreservación Verraco Cerdo Antioxidants DNA Spermatozoa Cryopreservation Boars Swine Melissa officinalis Esperma Ilex paraguariensis Mate Elissa officinalis Antioxidantes ADN Espermatozoo Criopreservación Verraco Cerdo Antioxidants DNA Spermatozoa Cryopreservation Boars Swine Melissa officinalis Esperma Luño, Victoria Gil, María Lydia Olaciregui, Maite Jerez, Rodrigo A. De Blas, José Ignacio Hozbor, Federico Andres Antioxidant effect of lemon balm (Melissa officinalis) and mate tea (Ilex paraguensys) on quality, lipid peroxidation and DNA oxidation of cryopreserved boar epididymal spermatozoa |
description |
In this study, we investigated the protective ability of the addition of two antioxidant herb extracts, mate tea and lemon balm, on boar epididymal frozen–thawed spermatozoa quality. Testes from mature boars were collected at local slaughterhouse, and sperm samples from epididymis were recovered by flushing. Spermatozoa were cryopreserved in lactose–egg yolk buffer supplemented with various concentrations of lemon balm and mate tea (0, 2.5, 5 and 10 g l−1) using the straw‐freezing procedure. Motion parameters, acrosome and plasma membrane integrity, lipoperoxidation levels and DNA oxidative damage (8‐hydroxy‐2′‐deoxyguanosine base lesion) were evaluated. There were no differences among experimental groups with regard to motility characteristics, viability, acrosome and plasma membrane integrity; however, the highest concentration of lemon balm produced significant (P < 0.05) improvement in curvilinear trajectory, straightness and amplitude of lateral head displacement after thawing. The supplementation of freezing extender with mate tea and lemon balm reduced sperm lipid membrane peroxidation, and only mate tea protected DNA against oxidative damage during cryopreservation at 120 min post‐thawing (P < 0.05). Mate tea experimental extender at concentration of 10 g l−1 showed the lowest percentage of sperm oxidised DNA and malondialdehyde generation; thus, mate tea is a potential candidate such as antioxidant compound on boar sperm cryopreservation medium. |
format |
info:ar-repo/semantics/artículo |
topic_facet |
Ilex paraguariensis Mate Elissa officinalis Antioxidantes ADN Espermatozoo Criopreservación Verraco Cerdo Antioxidants DNA Spermatozoa Cryopreservation Boars Swine Melissa officinalis Esperma |
author |
Luño, Victoria Gil, María Lydia Olaciregui, Maite Jerez, Rodrigo A. De Blas, José Ignacio Hozbor, Federico Andres |
author_facet |
Luño, Victoria Gil, María Lydia Olaciregui, Maite Jerez, Rodrigo A. De Blas, José Ignacio Hozbor, Federico Andres |
author_sort |
Luño, Victoria |
title |
Antioxidant effect of lemon balm (Melissa officinalis) and mate tea (Ilex paraguensys) on quality, lipid peroxidation and DNA oxidation of cryopreserved boar epididymal spermatozoa |
title_short |
Antioxidant effect of lemon balm (Melissa officinalis) and mate tea (Ilex paraguensys) on quality, lipid peroxidation and DNA oxidation of cryopreserved boar epididymal spermatozoa |
title_full |
Antioxidant effect of lemon balm (Melissa officinalis) and mate tea (Ilex paraguensys) on quality, lipid peroxidation and DNA oxidation of cryopreserved boar epididymal spermatozoa |
title_fullStr |
Antioxidant effect of lemon balm (Melissa officinalis) and mate tea (Ilex paraguensys) on quality, lipid peroxidation and DNA oxidation of cryopreserved boar epididymal spermatozoa |
title_full_unstemmed |
Antioxidant effect of lemon balm (Melissa officinalis) and mate tea (Ilex paraguensys) on quality, lipid peroxidation and DNA oxidation of cryopreserved boar epididymal spermatozoa |
title_sort |
antioxidant effect of lemon balm (melissa officinalis) and mate tea (ilex paraguensys) on quality, lipid peroxidation and dna oxidation of cryopreserved boar epididymal spermatozoa |
publisher |
Wiley |
publishDate |
2015-11 |
url |
https://onlinelibrary.wiley.com/doi/abs/10.1111/and.12370 http://hdl.handle.net/20.500.12123/4737 https://doi.org/10.1111/and.12370 |
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