Detection, molecular characterization and phylogenetic analysis of G3P[12] and G14P[12] equine rotavirus strains co-circulating in central Kentucky
Equine rotavirus A (ERVA) is the leading cause of diarrhea in neonatal foals and a major health problem to the equine breeding industry worldwide. The G3P[12] and G14P[12] ERVA genotypes are the most prevalent in foals with diarrhea. Control and prevention strategies include vaccination of pregnant mares with an inactivated vaccine containing a prototype ERVA G3P[12] strain with limited and controversial field efficacy. Here, we performed the molecular characterization of ERVA strains circulating in central Kentucky using fecal samples collected during the 2017 foaling season. The data indicated for the first time that the G14P[12] genotype is predominant in this region in contrast to a previous serotyping study where only G3 genotype strains were reported. Overall, analysis of antigenic sites in the VP7 protein demonstrated the presence of several amino acid substitutions in the epitopes exposed on the surface including a non-conserved N-linked glycosylation site (D123N) in G14P[12] strains, while changes in antigenic sites of VP8* were minor. Also, we report the successful isolation of three ERVA G14P[12] strains which presented a high identity with other G14 strains from around the world. These may constitute ideal reference strains to comparatively study the molecular biology of G3 and G14 strains and perform vaccine efficacy studies following heterologous challenge in the future.
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Format: | info:ar-repo/semantics/artículo biblioteca |
Language: | eng |
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Elsevier
2018
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Subjects: | Rotavirus, Caballos, Filogenia, Horses, Phylogeny, Foal Diarrhea, G3P, G14P, Equine Rotavirus, Kentucky, Estados Unidos, Diarrea del Potro, Rotavirus Equino, |
Online Access: | http://hdl.handle.net/20.500.12123/4448 https://www.sciencedirect.com/science/article/pii/S0168170218301382?via%3Dihub https://doi.org/10.1016/j.virusres.2018.05.025 |
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oai:localhost:20.500.12123-44482019-02-15T13:40:30Z Detection, molecular characterization and phylogenetic analysis of G3P[12] and G14P[12] equine rotavirus strains co-circulating in central Kentucky Carossino, Mariano Barrandeguy, Maria Edith Li, Yanqiu Parreño, Viviana Janes, Jennifer Loynachan, Alan T. Balasuriya, Udeni B.R. Rotavirus Caballos Filogenia Horses Phylogeny Foal Diarrhea G3P G14P Equine Rotavirus Kentucky, Estados Unidos Diarrea del Potro Rotavirus Equino Equine rotavirus A (ERVA) is the leading cause of diarrhea in neonatal foals and a major health problem to the equine breeding industry worldwide. The G3P[12] and G14P[12] ERVA genotypes are the most prevalent in foals with diarrhea. Control and prevention strategies include vaccination of pregnant mares with an inactivated vaccine containing a prototype ERVA G3P[12] strain with limited and controversial field efficacy. Here, we performed the molecular characterization of ERVA strains circulating in central Kentucky using fecal samples collected during the 2017 foaling season. The data indicated for the first time that the G14P[12] genotype is predominant in this region in contrast to a previous serotyping study where only G3 genotype strains were reported. Overall, analysis of antigenic sites in the VP7 protein demonstrated the presence of several amino acid substitutions in the epitopes exposed on the surface including a non-conserved N-linked glycosylation site (D123N) in G14P[12] strains, while changes in antigenic sites of VP8* were minor. Also, we report the successful isolation of three ERVA G14P[12] strains which presented a high identity with other G14 strains from around the world. These may constitute ideal reference strains to comparatively study the molecular biology of G3 and G14 strains and perform vaccine efficacy studies following heterologous challenge in the future. Instituto de Virología Fil: Carossino, Mariano. University of Kentucky. Department of Veterinary Science. Maxwell H. Gluck Equine Research Center; Estados Unidos.Universidad del Salvador. Escuela de Veterinaria; Argentina Fil: Barrandeguy, Maria Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Universidad del Salvador. Escuela de Veterinaria; Argentina Fil: Li, Yanqiu. University of Kentucky. Department of Veterinary Science. Maxwell H. Gluck Equine Research Center; Estados Unidos Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Janes, Jennifer. University of Kentucky. Department of Veterinary Science. University of Kentucky Veterinary Diagnostic Laboratory; Estados Unidos Fil: Loynachan, Alan T. University of Kentucky. Department of Veterinary Science. University of Kentucky Veterinary Diagnostic Laboratory; Estados Unidos Fil: Balasuriya, Udeni B.R. University of Kentucky. Department of Veterinary Science. Maxwell H. Gluck Equine Research Center; Estados Unidos.Universidad del Salvador. Escuela de Veterinaria; Argentina 2019-02-15T13:35:42Z 2019-02-15T13:35:42Z 2018 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/4448 https://www.sciencedirect.com/science/article/pii/S0168170218301382?via%3Dihub 0168-1702 https://doi.org/10.1016/j.virusres.2018.05.025 eng info:eu-repo/semantics/openAccess application/pdf Elsevier Virus research 255 : 39-54. (15 August 2018) |
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Rotavirus Caballos Filogenia Horses Phylogeny Foal Diarrhea G3P G14P Equine Rotavirus Kentucky, Estados Unidos Diarrea del Potro Rotavirus Equino Rotavirus Caballos Filogenia Horses Phylogeny Foal Diarrhea G3P G14P Equine Rotavirus Kentucky, Estados Unidos Diarrea del Potro Rotavirus Equino |
spellingShingle |
Rotavirus Caballos Filogenia Horses Phylogeny Foal Diarrhea G3P G14P Equine Rotavirus Kentucky, Estados Unidos Diarrea del Potro Rotavirus Equino Rotavirus Caballos Filogenia Horses Phylogeny Foal Diarrhea G3P G14P Equine Rotavirus Kentucky, Estados Unidos Diarrea del Potro Rotavirus Equino Carossino, Mariano Barrandeguy, Maria Edith Li, Yanqiu Parreño, Viviana Janes, Jennifer Loynachan, Alan T. Balasuriya, Udeni B.R. Detection, molecular characterization and phylogenetic analysis of G3P[12] and G14P[12] equine rotavirus strains co-circulating in central Kentucky |
description |
Equine rotavirus A (ERVA) is the leading cause of diarrhea in neonatal foals and a major health problem to the equine breeding industry worldwide. The G3P[12] and G14P[12] ERVA genotypes are the most prevalent in foals with diarrhea. Control and prevention strategies include vaccination of pregnant mares with an inactivated vaccine containing a prototype ERVA G3P[12] strain with limited and controversial field efficacy. Here, we performed the molecular characterization of ERVA strains circulating in central Kentucky using fecal samples collected during the 2017 foaling season. The data indicated for the first time that the G14P[12] genotype is predominant in this region in contrast to a previous serotyping study where only G3 genotype strains were reported. Overall, analysis of antigenic sites in the VP7 protein demonstrated the presence of several amino acid substitutions in the epitopes exposed on the surface including a non-conserved N-linked glycosylation site (D123N) in G14P[12] strains, while changes in antigenic sites of VP8* were minor. Also, we report the successful isolation of three ERVA G14P[12] strains which presented a high identity with other G14 strains from around the world. These may constitute ideal reference strains to comparatively study the molecular biology of G3 and G14 strains and perform vaccine efficacy studies following heterologous challenge in the future. |
format |
info:ar-repo/semantics/artículo |
topic_facet |
Rotavirus Caballos Filogenia Horses Phylogeny Foal Diarrhea G3P G14P Equine Rotavirus Kentucky, Estados Unidos Diarrea del Potro Rotavirus Equino |
author |
Carossino, Mariano Barrandeguy, Maria Edith Li, Yanqiu Parreño, Viviana Janes, Jennifer Loynachan, Alan T. Balasuriya, Udeni B.R. |
author_facet |
Carossino, Mariano Barrandeguy, Maria Edith Li, Yanqiu Parreño, Viviana Janes, Jennifer Loynachan, Alan T. Balasuriya, Udeni B.R. |
author_sort |
Carossino, Mariano |
title |
Detection, molecular characterization and phylogenetic analysis of G3P[12] and G14P[12] equine rotavirus strains co-circulating in central Kentucky |
title_short |
Detection, molecular characterization and phylogenetic analysis of G3P[12] and G14P[12] equine rotavirus strains co-circulating in central Kentucky |
title_full |
Detection, molecular characterization and phylogenetic analysis of G3P[12] and G14P[12] equine rotavirus strains co-circulating in central Kentucky |
title_fullStr |
Detection, molecular characterization and phylogenetic analysis of G3P[12] and G14P[12] equine rotavirus strains co-circulating in central Kentucky |
title_full_unstemmed |
Detection, molecular characterization and phylogenetic analysis of G3P[12] and G14P[12] equine rotavirus strains co-circulating in central Kentucky |
title_sort |
detection, molecular characterization and phylogenetic analysis of g3p[12] and g14p[12] equine rotavirus strains co-circulating in central kentucky |
publisher |
Elsevier |
publishDate |
2018 |
url |
http://hdl.handle.net/20.500.12123/4448 https://www.sciencedirect.com/science/article/pii/S0168170218301382?via%3Dihub https://doi.org/10.1016/j.virusres.2018.05.025 |
work_keys_str_mv |
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