Bean leafroll virus (BLRV) in Argentina: molecular characterization and detection in alfalfa fields
Alfalfa (Medicago sativa L.) is the main forage crop in Argentina and plays an essential role in the animal production chain, but it can be negatively affected by many factors, such as viral diseases. Here, we report the presence of Bean leafroll virus infecting alfalfa (BLRV-Arg), its distribution on alfalfa fields and its complete genome sequence. BLRV-Arg showed a prevalence of over 50 % and a wide distribution in Argentine alfalfa fields. The BLRV genome of the Manfredi isolate (Manfredi BLRV-Arg) is 5884 nucleotides in length, with a genome organization similar to that already reported for this virus. The complete nucleotide sequence or selected genes were subjected to sequence comparisons. Manfredi BLRV-Arg had more than 96 % nucleotide and amino acid sequence identities, both for complete genome sequence and ORFs, with those of the two full-length genome sequences available in GenBank. The complete CP sequences of Manfredi BLRV-Arg showed an identity percentage over 98 % at nt and deduced aa level when were compared with those of the other CP complete sequences available in GenBank. This is the first evidence for the presence of BLRV in Argentina, its geographical distribution in alfalfa production areas and the complete genome sequence of an isolate from alfalfa as natural host.
Main Authors: | , , , , |
---|---|
Format: | info:ar-repo/semantics/artículo biblioteca |
Language: | eng |
Published: |
Springer
2016-09
|
Subjects: | Medicago Sativa, Luteovirus, Genomas, Distribución Geográfica, Bean Leafroll Virus, Genomes, Geographical Distribution, Alfalfa, Argentina, Secuenciación, BLRV, Sequencing, Field Detection, |
Online Access: | http://hdl.handle.net/20.500.12123/3655 https://link.springer.com/article/10.1007%2Fs10658-016-0899-5 https://doi.org/10.1007/s10658-016-0899-5 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:localhost:20.500.12123-3655 |
---|---|
record_format |
koha |
spelling |
oai:localhost:20.500.12123-36552018-10-22T12:20:21Z Bean leafroll virus (BLRV) in Argentina: molecular characterization and detection in alfalfa fields Trucco, Veronica Milagros De Breuil, Soledad Bejerman, Nicolas Esteban Lenardon, Sergio Luis Giolitti, Fabian Medicago Sativa Luteovirus Genomas Distribución Geográfica Bean Leafroll Virus Genomes Geographical Distribution Alfalfa Argentina Secuenciación BLRV Sequencing Field Detection Alfalfa (Medicago sativa L.) is the main forage crop in Argentina and plays an essential role in the animal production chain, but it can be negatively affected by many factors, such as viral diseases. Here, we report the presence of Bean leafroll virus infecting alfalfa (BLRV-Arg), its distribution on alfalfa fields and its complete genome sequence. BLRV-Arg showed a prevalence of over 50 % and a wide distribution in Argentine alfalfa fields. The BLRV genome of the Manfredi isolate (Manfredi BLRV-Arg) is 5884 nucleotides in length, with a genome organization similar to that already reported for this virus. The complete nucleotide sequence or selected genes were subjected to sequence comparisons. Manfredi BLRV-Arg had more than 96 % nucleotide and amino acid sequence identities, both for complete genome sequence and ORFs, with those of the two full-length genome sequences available in GenBank. The complete CP sequences of Manfredi BLRV-Arg showed an identity percentage over 98 % at nt and deduced aa level when were compared with those of the other CP complete sequences available in GenBank. This is the first evidence for the presence of BLRV in Argentina, its geographical distribution in alfalfa production areas and the complete genome sequence of an isolate from alfalfa as natural host. Instituto de Patología Vegetal Fil: Trucco, Veronica Milagros. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina 2018-10-22T11:34:28Z 2018-10-22T11:34:28Z 2016-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/3655 https://link.springer.com/article/10.1007%2Fs10658-016-0899-5 0929-1873 https://doi.org/10.1007/s10658-016-0899-5 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer European journal of plant pathology 146 (1) : 207–212. (September 2016) |
institution |
INTA AR |
collection |
DSpace |
country |
Argentina |
countrycode |
AR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-inta-ar |
tag |
biblioteca |
region |
America del Sur |
libraryname |
Biblioteca Central del INTA Argentina |
language |
eng |
topic |
Medicago Sativa Luteovirus Genomas Distribución Geográfica Bean Leafroll Virus Genomes Geographical Distribution Alfalfa Argentina Secuenciación BLRV Sequencing Field Detection Medicago Sativa Luteovirus Genomas Distribución Geográfica Bean Leafroll Virus Genomes Geographical Distribution Alfalfa Argentina Secuenciación BLRV Sequencing Field Detection |
spellingShingle |
Medicago Sativa Luteovirus Genomas Distribución Geográfica Bean Leafroll Virus Genomes Geographical Distribution Alfalfa Argentina Secuenciación BLRV Sequencing Field Detection Medicago Sativa Luteovirus Genomas Distribución Geográfica Bean Leafroll Virus Genomes Geographical Distribution Alfalfa Argentina Secuenciación BLRV Sequencing Field Detection Trucco, Veronica Milagros De Breuil, Soledad Bejerman, Nicolas Esteban Lenardon, Sergio Luis Giolitti, Fabian Bean leafroll virus (BLRV) in Argentina: molecular characterization and detection in alfalfa fields |
description |
Alfalfa (Medicago sativa L.) is the main forage crop in Argentina and plays an essential role in the animal production chain, but it can be negatively affected by many factors, such as viral diseases. Here, we report the presence of Bean leafroll virus infecting alfalfa (BLRV-Arg), its distribution on alfalfa fields and its complete genome sequence. BLRV-Arg showed a prevalence of over 50 % and a wide distribution in Argentine alfalfa fields. The BLRV genome of the Manfredi isolate (Manfredi BLRV-Arg) is 5884 nucleotides in length, with a genome organization similar to that already reported for this virus. The complete nucleotide sequence or selected genes were subjected to sequence comparisons. Manfredi BLRV-Arg had more than 96 % nucleotide and amino acid sequence identities, both for complete genome sequence and ORFs, with those of the two full-length genome sequences available in GenBank. The complete CP sequences of Manfredi BLRV-Arg showed an identity percentage over 98 % at nt and deduced aa level when were compared with those of the other CP complete sequences available in GenBank. This is the first evidence for the presence of BLRV in Argentina, its geographical distribution in alfalfa production areas and the complete genome sequence of an isolate from alfalfa as natural host. |
format |
info:ar-repo/semantics/artículo |
topic_facet |
Medicago Sativa Luteovirus Genomas Distribución Geográfica Bean Leafroll Virus Genomes Geographical Distribution Alfalfa Argentina Secuenciación BLRV Sequencing Field Detection |
author |
Trucco, Veronica Milagros De Breuil, Soledad Bejerman, Nicolas Esteban Lenardon, Sergio Luis Giolitti, Fabian |
author_facet |
Trucco, Veronica Milagros De Breuil, Soledad Bejerman, Nicolas Esteban Lenardon, Sergio Luis Giolitti, Fabian |
author_sort |
Trucco, Veronica Milagros |
title |
Bean leafroll virus (BLRV) in Argentina: molecular characterization and detection in alfalfa fields |
title_short |
Bean leafroll virus (BLRV) in Argentina: molecular characterization and detection in alfalfa fields |
title_full |
Bean leafroll virus (BLRV) in Argentina: molecular characterization and detection in alfalfa fields |
title_fullStr |
Bean leafroll virus (BLRV) in Argentina: molecular characterization and detection in alfalfa fields |
title_full_unstemmed |
Bean leafroll virus (BLRV) in Argentina: molecular characterization and detection in alfalfa fields |
title_sort |
bean leafroll virus (blrv) in argentina: molecular characterization and detection in alfalfa fields |
publisher |
Springer |
publishDate |
2016-09 |
url |
http://hdl.handle.net/20.500.12123/3655 https://link.springer.com/article/10.1007%2Fs10658-016-0899-5 https://doi.org/10.1007/s10658-016-0899-5 |
work_keys_str_mv |
AT truccoveronicamilagros beanleafrollvirusblrvinargentinamolecularcharacterizationanddetectioninalfalfafields AT debreuilsoledad beanleafrollvirusblrvinargentinamolecularcharacterizationanddetectioninalfalfafields AT bejermannicolasesteban beanleafrollvirusblrvinargentinamolecularcharacterizationanddetectioninalfalfafields AT lenardonsergioluis beanleafrollvirusblrvinargentinamolecularcharacterizationanddetectioninalfalfafields AT giolittifabian beanleafrollvirusblrvinargentinamolecularcharacterizationanddetectioninalfalfafields |
_version_ |
1756007283529089025 |