Molecular detection of Candidatus Ehrlichia pampeana (Rickettsiales: Anaplasmataceae) in Haemaphysalis juxtakochi (Acari: Ixodidae) from central Argentina
The aim of this study was to report the first detection of Candidatus Ehrlichia pampeana in Haemaphysalis juxtakochi from Argentina. Free-living ticks were collected from vegetation by drag-flag method on five sample sites in Entre Ríos Province, central Argentina, belonging to the Espinal Phytogeographic Province. Molecular detection of order Rickettsiales agents was performed using gltA (Rickettsia spp.), 16 S rRNA and groEL (Anaplasmataceae) genes as targets. A total of 67 ticks of Amblyomma aureolatum (20 nymphs and 4 adults), Amblyomma dubitatum (15 nymphs) and Haemaphysalis juxtakochi (24 nymphs and 4 adults) were collected. While all tested ticks were negative for Rickettsia spp., and Anaplasmataceae agents could neither be detected in A. aureolatum nor in A. dubitatum, Candidatus Ehrlichia pampeana was detected in one male of H. juxtakochi. DNA sequences of this microorganism (16 S rDNA and groEL) are related to sequences of Ehrlichia ewingii. The findings of the current study represent the first report of this Ehrlichia strain for Argentina.
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Format: | info:ar-repo/semantics/artículo biblioteca |
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Springer
2024-02
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Subjects: | Ehrlichia, Ixodidae, Haemaphysalis, Amblyomma, Identification, Identificación, Argentina, Ehrlichia pampeana, Haemaphysalis juxtakochi, Garrapatas, Ticks, |
Online Access: | http://hdl.handle.net/20.500.12123/16746 https://link.springer.com/article/10.1007/s11259-023-10219-6 https://doi.org/10.1007/s11259-023-10219-6 |
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oai:localhost:20.500.12123-167462024-02-22T10:54:51Z Molecular detection of Candidatus Ehrlichia pampeana (Rickettsiales: Anaplasmataceae) in Haemaphysalis juxtakochi (Acari: Ixodidae) from central Argentina Flores, Fernando Sebastian Sebastian, Patrick Stephan Nava, Santiago Ehrlichia Ixodidae Haemaphysalis Amblyomma Identification Identificación Argentina Ehrlichia pampeana Haemaphysalis juxtakochi Garrapatas Ticks The aim of this study was to report the first detection of Candidatus Ehrlichia pampeana in Haemaphysalis juxtakochi from Argentina. Free-living ticks were collected from vegetation by drag-flag method on five sample sites in Entre Ríos Province, central Argentina, belonging to the Espinal Phytogeographic Province. Molecular detection of order Rickettsiales agents was performed using gltA (Rickettsia spp.), 16 S rRNA and groEL (Anaplasmataceae) genes as targets. A total of 67 ticks of Amblyomma aureolatum (20 nymphs and 4 adults), Amblyomma dubitatum (15 nymphs) and Haemaphysalis juxtakochi (24 nymphs and 4 adults) were collected. While all tested ticks were negative for Rickettsia spp., and Anaplasmataceae agents could neither be detected in A. aureolatum nor in A. dubitatum, Candidatus Ehrlichia pampeana was detected in one male of H. juxtakochi. DNA sequences of this microorganism (16 S rDNA and groEL) are related to sequences of Ehrlichia ewingii. The findings of the current study represent the first report of this Ehrlichia strain for Argentina. EEA Rafaela Fil: Flores, Fernando S. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas (IIByT); Argentina Fil: Flores, Fernando S. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba (CIEC); Argentina Fil: Sebastian, Patrick Stephan. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Sebastian, Patrick Stephan. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina 2024-02-22T10:50:10Z 2024-02-22T10:50:10Z 2024-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16746 https://link.springer.com/article/10.1007/s11259-023-10219-6 0165-7380 1573-7446 https://doi.org/10.1007/s11259-023-10219-6 eng info:eu-repograntAgreement/INTA/2019-PE-E5-I109-001, Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y la info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Argentina .......... (nation) (World, South America) 7006477 Springer Veterinary Research Communications 48 (1) : 585-589 (February 2024) |
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Ehrlichia Ixodidae Haemaphysalis Amblyomma Identification Identificación Argentina Ehrlichia pampeana Haemaphysalis juxtakochi Garrapatas Ticks Ehrlichia Ixodidae Haemaphysalis Amblyomma Identification Identificación Argentina Ehrlichia pampeana Haemaphysalis juxtakochi Garrapatas Ticks |
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Ehrlichia Ixodidae Haemaphysalis Amblyomma Identification Identificación Argentina Ehrlichia pampeana Haemaphysalis juxtakochi Garrapatas Ticks Ehrlichia Ixodidae Haemaphysalis Amblyomma Identification Identificación Argentina Ehrlichia pampeana Haemaphysalis juxtakochi Garrapatas Ticks Flores, Fernando Sebastian Sebastian, Patrick Stephan Nava, Santiago Molecular detection of Candidatus Ehrlichia pampeana (Rickettsiales: Anaplasmataceae) in Haemaphysalis juxtakochi (Acari: Ixodidae) from central Argentina |
description |
The aim of this study was to report the first detection of Candidatus Ehrlichia pampeana in Haemaphysalis juxtakochi from Argentina. Free-living ticks were collected from vegetation by drag-flag method on five sample sites in Entre Ríos Province, central Argentina, belonging to the Espinal Phytogeographic Province. Molecular detection of order Rickettsiales agents was performed using gltA (Rickettsia spp.), 16 S rRNA and groEL (Anaplasmataceae) genes as targets. A total of 67 ticks of Amblyomma aureolatum (20 nymphs and 4 adults), Amblyomma dubitatum (15 nymphs) and Haemaphysalis juxtakochi (24 nymphs and 4 adults) were collected. While all tested ticks were negative for Rickettsia spp., and Anaplasmataceae agents could neither be detected in A. aureolatum nor in A. dubitatum, Candidatus Ehrlichia pampeana was detected in one male of H. juxtakochi. DNA sequences of this microorganism (16 S rDNA and groEL) are related to sequences of Ehrlichia ewingii. The findings of the current study represent the first report of this Ehrlichia strain for Argentina. |
format |
info:ar-repo/semantics/artículo |
topic_facet |
Ehrlichia Ixodidae Haemaphysalis Amblyomma Identification Identificación Argentina Ehrlichia pampeana Haemaphysalis juxtakochi Garrapatas Ticks |
author |
Flores, Fernando Sebastian Sebastian, Patrick Stephan Nava, Santiago |
author_facet |
Flores, Fernando Sebastian Sebastian, Patrick Stephan Nava, Santiago |
author_sort |
Flores, Fernando Sebastian |
title |
Molecular detection of Candidatus Ehrlichia pampeana (Rickettsiales: Anaplasmataceae) in Haemaphysalis juxtakochi (Acari: Ixodidae) from central Argentina |
title_short |
Molecular detection of Candidatus Ehrlichia pampeana (Rickettsiales: Anaplasmataceae) in Haemaphysalis juxtakochi (Acari: Ixodidae) from central Argentina |
title_full |
Molecular detection of Candidatus Ehrlichia pampeana (Rickettsiales: Anaplasmataceae) in Haemaphysalis juxtakochi (Acari: Ixodidae) from central Argentina |
title_fullStr |
Molecular detection of Candidatus Ehrlichia pampeana (Rickettsiales: Anaplasmataceae) in Haemaphysalis juxtakochi (Acari: Ixodidae) from central Argentina |
title_full_unstemmed |
Molecular detection of Candidatus Ehrlichia pampeana (Rickettsiales: Anaplasmataceae) in Haemaphysalis juxtakochi (Acari: Ixodidae) from central Argentina |
title_sort |
molecular detection of candidatus ehrlichia pampeana (rickettsiales: anaplasmataceae) in haemaphysalis juxtakochi (acari: ixodidae) from central argentina |
publisher |
Springer |
publishDate |
2024-02 |
url |
http://hdl.handle.net/20.500.12123/16746 https://link.springer.com/article/10.1007/s11259-023-10219-6 https://doi.org/10.1007/s11259-023-10219-6 |
work_keys_str_mv |
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