Modulation of granzymes mRNA expression in neural tissue of BoAHV-1 and BoAHV-5-infected cattle
Bovine alphaherpesviruses (BoAHV)-1 and 5 are neurotropic viruses of cattle which differ in their neuropathogenic potential. BoAHV-5 is responsible for non-suppurative meningoencephalitis in calves while BoAHV-1 can occasionally cause encephalitis. Granzymes (GZMs) are serine-proteases that participate in CD8+ T cells-mediated killing of virally-infected cells upon release through perforin (PFN)-formed pores in the cell membrane. Recently, six GZMs have been identified in cattle (A, B, K, H, M and O). However, their expression in bovine tissues has not been evaluated. In this study, the mRNA expression levels of PFN and GZMs A, B, K, H and M in the nervous system of calves experimentally-inoculated with BoAHV-1 or BoAHV-5 were analyzed at the three distinct stages of the infectious cycle of alphaherpesviruses: acute infection, latency and reactivation. This is the first report describing the expression of GZMs in bovine neural tissue and the first analysis of GZM expression in the context of bovine alphaherpesviruses neuropathogenesis. The findings revealed that PFN and GZM K are upregulated during BoAHV-1 or BoAHV-5 acute infection. In contrast to BoAHV-1, during BoAHV-5 latency a significant up-regulation of PFN, GZM K and GZM H was detected. PFN and GZM A, K and H expression was also up-regulated during BoAHV-5 reactivation. Therefore, a distinct pattern of PFN and GZM expression is evident along the infectious cycle of each alphaherpesvirus and this might contribute to the differences in BoAHV-1 and BoAHV-5 neuropathogenesis.
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Elsevier
2023-06
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Subjects: | Ganado Bovino, Herpes Virus Bovino, Tejido Nervioso, Cattle, Bovine Herpesvirus, Nervous Tissues, |
Online Access: | http://hdl.handle.net/20.500.12123/16012 https://www.sciencedirect.com/science/article/pii/S0165242723000648 https://doi.org/10.1016/j.vetimm.2023.110610 |
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oai:localhost:20.500.12123-160122023-11-27T10:43:04Z Modulation of granzymes mRNA expression in neural tissue of BoAHV-1 and BoAHV-5-infected cattle Martínez Cuesta, Lucía Verna, Andrea Elizabeth Pérez, Sandra Ganado Bovino Herpes Virus Bovino Tejido Nervioso Cattle Bovine Herpesvirus Nervous Tissues Bovine alphaherpesviruses (BoAHV)-1 and 5 are neurotropic viruses of cattle which differ in their neuropathogenic potential. BoAHV-5 is responsible for non-suppurative meningoencephalitis in calves while BoAHV-1 can occasionally cause encephalitis. Granzymes (GZMs) are serine-proteases that participate in CD8+ T cells-mediated killing of virally-infected cells upon release through perforin (PFN)-formed pores in the cell membrane. Recently, six GZMs have been identified in cattle (A, B, K, H, M and O). However, their expression in bovine tissues has not been evaluated. In this study, the mRNA expression levels of PFN and GZMs A, B, K, H and M in the nervous system of calves experimentally-inoculated with BoAHV-1 or BoAHV-5 were analyzed at the three distinct stages of the infectious cycle of alphaherpesviruses: acute infection, latency and reactivation. This is the first report describing the expression of GZMs in bovine neural tissue and the first analysis of GZM expression in the context of bovine alphaherpesviruses neuropathogenesis. The findings revealed that PFN and GZM K are upregulated during BoAHV-1 or BoAHV-5 acute infection. In contrast to BoAHV-1, during BoAHV-5 latency a significant up-regulation of PFN, GZM K and GZM H was detected. PFN and GZM A, K and H expression was also up-regulated during BoAHV-5 reactivation. Therefore, a distinct pattern of PFN and GZM expression is evident along the infectious cycle of each alphaherpesvirus and this might contribute to the differences in BoAHV-1 and BoAHV-5 neuropathogenesis. EEA Balcarce Fil: Martínez Cuesta, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. Fil: Verna, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Pérez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Centro de Investigación Veterinaria de Tandil; Argentina. 2023-11-27T10:39:24Z 2023-11-27T10:39:24Z 2023-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16012 https://www.sciencedirect.com/science/article/pii/S0165242723000648 1873-2534 (Online) 0165-2427 (Print) https://doi.org/10.1016/j.vetimm.2023.110610 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Veterinary Immunology and Immunopathology 260 : 110610 (June 2023) |
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Ganado Bovino Herpes Virus Bovino Tejido Nervioso Cattle Bovine Herpesvirus Nervous Tissues Ganado Bovino Herpes Virus Bovino Tejido Nervioso Cattle Bovine Herpesvirus Nervous Tissues |
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Ganado Bovino Herpes Virus Bovino Tejido Nervioso Cattle Bovine Herpesvirus Nervous Tissues Ganado Bovino Herpes Virus Bovino Tejido Nervioso Cattle Bovine Herpesvirus Nervous Tissues Martínez Cuesta, Lucía Verna, Andrea Elizabeth Pérez, Sandra Modulation of granzymes mRNA expression in neural tissue of BoAHV-1 and BoAHV-5-infected cattle |
description |
Bovine alphaherpesviruses (BoAHV)-1 and 5 are neurotropic viruses of cattle which differ in their neuropathogenic potential. BoAHV-5 is responsible for non-suppurative meningoencephalitis in calves while BoAHV-1 can occasionally cause encephalitis. Granzymes (GZMs) are serine-proteases that participate in CD8+ T cells-mediated killing of virally-infected cells upon release through perforin (PFN)-formed pores in the cell membrane. Recently, six GZMs have been identified in cattle (A, B, K, H, M and O). However, their expression in bovine tissues has not been evaluated. In this study, the mRNA expression levels of PFN and GZMs A, B, K, H and M in the nervous system of calves experimentally-inoculated with BoAHV-1 or BoAHV-5 were analyzed at the three distinct stages of the infectious cycle of alphaherpesviruses: acute infection, latency and reactivation. This is the first report describing the expression of GZMs in bovine neural tissue and the first analysis of GZM expression in the context of bovine alphaherpesviruses neuropathogenesis. The findings revealed that PFN and GZM K are upregulated during BoAHV-1 or BoAHV-5 acute infection. In contrast to BoAHV-1, during BoAHV-5 latency a significant up-regulation of PFN, GZM K and GZM H was detected. PFN and GZM A, K and H expression was also up-regulated during BoAHV-5 reactivation. Therefore, a distinct pattern of PFN and GZM expression is evident along the infectious cycle of each alphaherpesvirus and this might contribute to the differences in BoAHV-1 and BoAHV-5 neuropathogenesis. |
format |
info:ar-repo/semantics/artículo |
topic_facet |
Ganado Bovino Herpes Virus Bovino Tejido Nervioso Cattle Bovine Herpesvirus Nervous Tissues |
author |
Martínez Cuesta, Lucía Verna, Andrea Elizabeth Pérez, Sandra |
author_facet |
Martínez Cuesta, Lucía Verna, Andrea Elizabeth Pérez, Sandra |
author_sort |
Martínez Cuesta, Lucía |
title |
Modulation of granzymes mRNA expression in neural tissue of BoAHV-1 and BoAHV-5-infected cattle |
title_short |
Modulation of granzymes mRNA expression in neural tissue of BoAHV-1 and BoAHV-5-infected cattle |
title_full |
Modulation of granzymes mRNA expression in neural tissue of BoAHV-1 and BoAHV-5-infected cattle |
title_fullStr |
Modulation of granzymes mRNA expression in neural tissue of BoAHV-1 and BoAHV-5-infected cattle |
title_full_unstemmed |
Modulation of granzymes mRNA expression in neural tissue of BoAHV-1 and BoAHV-5-infected cattle |
title_sort |
modulation of granzymes mrna expression in neural tissue of boahv-1 and boahv-5-infected cattle |
publisher |
Elsevier |
publishDate |
2023-06 |
url |
http://hdl.handle.net/20.500.12123/16012 https://www.sciencedirect.com/science/article/pii/S0165242723000648 https://doi.org/10.1016/j.vetimm.2023.110610 |
work_keys_str_mv |
AT martinezcuestalucia modulationofgranzymesmrnaexpressioninneuraltissueofboahv1andboahv5infectedcattle AT vernaandreaelizabeth modulationofgranzymesmrnaexpressioninneuraltissueofboahv1andboahv5infectedcattle AT perezsandra modulationofgranzymesmrnaexpressioninneuraltissueofboahv1andboahv5infectedcattle |
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1798158394978205696 |