Assessment of tuberculosis biomarkers in paratuberculosis-infected cattle

Assessment of tuberculosis biomarkers in paratuberculosis-infected cattle

Introduction: Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis, respectively the causative agents of bovine tuberculosis (bTB) and bovine paratuberculosis (PTB), share a high number of antigenic proteins. This characteristics makes the differential diagnosis of the diseases difficult. The interferon gamma (IFN-γ), C-X-C motif chemokine ligand 10 (CXCL10), matrix metallopeptidase 9 (MMP9), interleukin 22 (IL-22) and thrombospondin 1 (THBS1) bovine genes have already been shown to be accurate transcriptional biomarkers of bTB. In order to improve the diagnosis of bTB and PTB, in the present study we evaluated the risk of false positivity of these bTB biomarkers in cattle with PTB. Material and methods: The transcription of these genes was studied in 13 PTB-infected cattle, using Mycobacterium avium subsp. paratuberculosis (MAP)-stimulated peripheral blood mononuclear cells (PBMC). Results: Overall, the levels of IFN-γ, CXCL10, MMP9 and IL-22 transcripts in MAP-stimulated PBMC failed to differentiate animals with PTB from healthy animals. However, as bTB-afflicted cattle do, the MAP-infected group also displayed a lower level of THBS1 transcription than the non-infected animals. Conclusion: The results of this study add new specificity attributes to the levels of transcription of IFN-γ, CXCL10, MMP9 and IL-22 as biomarkers for bTB.

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Main Authors: Klepp, Laura Ines, Colombatti Olivieri, Maria Alejandra, Moyano, Roberto Damian, Romano, Maria Isabel, Malovrh, Tadej, Ocepek, Matjaž, Blanco, Federico Carlos, Bigi, Fabiana
Format: info:ar-repo/semantics/artículo biblioteca
Language:eng
Published: Sciendo 2023-03
Subjects:Bovine Tuberculosis, Paratuberculosis, Cattle, Tuberculosis Bovina, Ganado Bovino, Biomarkers, Biomarcadores,
Online Access:http://hdl.handle.net/20.500.12123/15159
https://sciendo.com/article/10.2478/jvetres-2023-0007
https://doi.org/10.2478/jvetres-2023-0007
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record_format koha
institution INTA AR
collection DSpace
country Argentina
countrycode AR
component Bibliográfico
access En linea
databasecode dig-inta-ar
tag biblioteca
region America del Sur
libraryname Biblioteca Central del INTA Argentina
language eng
topic Bovine Tuberculosis
Paratuberculosis
Cattle
Tuberculosis Bovina
Ganado Bovino
Biomarkers
Biomarcadores
Bovine Tuberculosis
Paratuberculosis
Cattle
Tuberculosis Bovina
Ganado Bovino
Biomarkers
Biomarcadores
spellingShingle Bovine Tuberculosis
Paratuberculosis
Cattle
Tuberculosis Bovina
Ganado Bovino
Biomarkers
Biomarcadores
Bovine Tuberculosis
Paratuberculosis
Cattle
Tuberculosis Bovina
Ganado Bovino
Biomarkers
Biomarcadores
Klepp, Laura Ines
Colombatti Olivieri, Maria Alejandra
Moyano, Roberto Damian
Romano, Maria Isabel
Malovrh, Tadej
Ocepek, Matjaž
Blanco, Federico Carlos
Bigi, Fabiana
Assessment of tuberculosis biomarkers in paratuberculosis-infected cattle
description Introduction: Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis, respectively the causative agents of bovine tuberculosis (bTB) and bovine paratuberculosis (PTB), share a high number of antigenic proteins. This characteristics makes the differential diagnosis of the diseases difficult. The interferon gamma (IFN-γ), C-X-C motif chemokine ligand 10 (CXCL10), matrix metallopeptidase 9 (MMP9), interleukin 22 (IL-22) and thrombospondin 1 (THBS1) bovine genes have already been shown to be accurate transcriptional biomarkers of bTB. In order to improve the diagnosis of bTB and PTB, in the present study we evaluated the risk of false positivity of these bTB biomarkers in cattle with PTB. Material and methods: The transcription of these genes was studied in 13 PTB-infected cattle, using Mycobacterium avium subsp. paratuberculosis (MAP)-stimulated peripheral blood mononuclear cells (PBMC). Results: Overall, the levels of IFN-γ, CXCL10, MMP9 and IL-22 transcripts in MAP-stimulated PBMC failed to differentiate animals with PTB from healthy animals. However, as bTB-afflicted cattle do, the MAP-infected group also displayed a lower level of THBS1 transcription than the non-infected animals. Conclusion: The results of this study add new specificity attributes to the levels of transcription of IFN-γ, CXCL10, MMP9 and IL-22 as biomarkers for bTB.
format info:ar-repo/semantics/artículo
topic_facet Bovine Tuberculosis
Paratuberculosis
Cattle
Tuberculosis Bovina
Ganado Bovino
Biomarkers
Biomarcadores
author Klepp, Laura Ines
Colombatti Olivieri, Maria Alejandra
Moyano, Roberto Damian
Romano, Maria Isabel
Malovrh, Tadej
Ocepek, Matjaž
Blanco, Federico Carlos
Bigi, Fabiana
author_facet Klepp, Laura Ines
Colombatti Olivieri, Maria Alejandra
Moyano, Roberto Damian
Romano, Maria Isabel
Malovrh, Tadej
Ocepek, Matjaž
Blanco, Federico Carlos
Bigi, Fabiana
author_sort Klepp, Laura Ines
title Assessment of tuberculosis biomarkers in paratuberculosis-infected cattle
title_short Assessment of tuberculosis biomarkers in paratuberculosis-infected cattle
title_full Assessment of tuberculosis biomarkers in paratuberculosis-infected cattle
title_fullStr Assessment of tuberculosis biomarkers in paratuberculosis-infected cattle
title_full_unstemmed Assessment of tuberculosis biomarkers in paratuberculosis-infected cattle
title_sort assessment of tuberculosis biomarkers in paratuberculosis-infected cattle
publisher Sciendo
publishDate 2023-03
url http://hdl.handle.net/20.500.12123/15159
https://sciendo.com/article/10.2478/jvetres-2023-0007
https://doi.org/10.2478/jvetres-2023-0007
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spelling oai:localhost:20.500.12123-151592023-09-11T11:05:19Z Assessment of tuberculosis biomarkers in paratuberculosis-infected cattle Klepp, Laura Ines Colombatti Olivieri, Maria Alejandra Moyano, Roberto Damian Romano, Maria Isabel Malovrh, Tadej Ocepek, Matjaž Blanco, Federico Carlos Bigi, Fabiana Bovine Tuberculosis Paratuberculosis Cattle Tuberculosis Bovina Ganado Bovino Biomarkers Biomarcadores Introduction: Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis, respectively the causative agents of bovine tuberculosis (bTB) and bovine paratuberculosis (PTB), share a high number of antigenic proteins. This characteristics makes the differential diagnosis of the diseases difficult. The interferon gamma (IFN-γ), C-X-C motif chemokine ligand 10 (CXCL10), matrix metallopeptidase 9 (MMP9), interleukin 22 (IL-22) and thrombospondin 1 (THBS1) bovine genes have already been shown to be accurate transcriptional biomarkers of bTB. In order to improve the diagnosis of bTB and PTB, in the present study we evaluated the risk of false positivity of these bTB biomarkers in cattle with PTB. Material and methods: The transcription of these genes was studied in 13 PTB-infected cattle, using Mycobacterium avium subsp. paratuberculosis (MAP)-stimulated peripheral blood mononuclear cells (PBMC). Results: Overall, the levels of IFN-γ, CXCL10, MMP9 and IL-22 transcripts in MAP-stimulated PBMC failed to differentiate animals with PTB from healthy animals. However, as bTB-afflicted cattle do, the MAP-infected group also displayed a lower level of THBS1 transcription than the non-infected animals. Conclusion: The results of this study add new specificity attributes to the levels of transcription of IFN-γ, CXCL10, MMP9 and IL-22 as biomarkers for bTB. Instituto de Biotecnología Fil: Klepp, Laura Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Klepp, Laura Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Colombatti Olivieri, Maria Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Colombatti Olivieri, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Moyano, Roberto Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Malovrh, Tadej. University of Ljubljana. Veterinary Faculty. Institute for Microbiology and Parasitology; Eslovenia Fil: Ocepek, Matjaž. University of Ljubljana. Veterinary Faculty. Institute for Microbiology and Parasitology; Eslovenia Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Bigi, Fabiana. Consejo Nacional de investigaciones Científicas y Tecnológicas; Argentina 2023-09-11T10:59:25Z 2023-09-11T10:59:25Z 2023-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15159 https://sciendo.com/article/10.2478/jvetres-2023-0007 2251-6190 https://doi.org/10.2478/jvetres-2023-0007 eng info:eu-repograntAgreement/INTA/2019-PD-E5-I103-001, Desarrollo de tecnologías diagnósticas y estudios epidemiológicos para el control de enfermedades que afectan la producción animal y la salud pública info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Sciendo Journal of Veterinary Research 67 (1) : 55-60 (March 2023)

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