Cytokine expression profile of B. melitensis-infected goat monocyte-derived macrophages
Brucella parasitize the macrophage where is able to replicate and modulate the immune response in order to establish a chronic infection. The most adequate response to control and eliminate Brucella infection is a type 1 (Th1) cell-mediated effector immunity. Research in immune response of B. melitensis-infected goats is relatively scarce. In this study, we first evaluated changes in the gene expression of cytokines, a chemokine (CCL2) and the inducible nitric oxide synthase (iNOS) of goat macrophage cultures derived from monocytes (MDMs) infected for 4 and 24 h with Brucella melitensis strain 16 M. TNFα, IL-1β and iNOS, and IL-12p40, IFNγ and also iNOS were significantly expressed (p < 0.05) at 4 and 24 h respectively, in infected compared to non-infected MDMs. Therefore, the in vitro challenge of goat MDMs with B. melitensis promoted a transcriptional profile consistent with a type 1 response. However, when the immune response to B. melitensis infection was contrasted between MDM cultures phenotypically restrictive or permissive to intracellular multiplication of B. melitensis 16 M, it was observed that the relative IL-4 mRNA expression was significantly higher in permissive macrophage cultures with respect to restrictive cultures (p < 0.05), independently of the time p.i. A similar trend, although non-statistical, was recorded for IL-10, but not for pro-inflammatory cytokines. Thus, the up-expression profile of inhibitory instead of pro-inflammatory cytokines could explain, in part, the difference observed in the ability to restrict intracellular replication of Brucella. In this sense, the present results make a significant contribution to the knowledge of the immune response induced by B. melitensis in macrophages of its preferential host species.
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Elsevier
2023-05
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Subjects: | Brucella melitensis, Citoquinas, Genética, Caprinos, Respuesta Inmunológica, Brucelosis, Macrofagos, Cytokines, Genetics, Goats, Immune Response, Brucellosis, Macrophages, |
Online Access: | http://hdl.handle.net/20.500.12123/14473 https://www.sciencedirect.com/science/article/pii/S0171298523000438 https://doi.org/10.1016/j.imbio.2023.152375 |
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oai:localhost:20.500.12123-144732023-04-13T16:48:05Z Cytokine expression profile of B. melitensis-infected goat monocyte-derived macrophages Maurizio, Estefanía Rossi, Ursula Amaranta Trangoni, Marcos David Rossetti, Carlos Alberto Brucella melitensis Citoquinas Genética Caprinos Respuesta Inmunológica Brucelosis Macrofagos Cytokines Genetics Goats Immune Response Brucellosis Macrophages Brucella parasitize the macrophage where is able to replicate and modulate the immune response in order to establish a chronic infection. The most adequate response to control and eliminate Brucella infection is a type 1 (Th1) cell-mediated effector immunity. Research in immune response of B. melitensis-infected goats is relatively scarce. In this study, we first evaluated changes in the gene expression of cytokines, a chemokine (CCL2) and the inducible nitric oxide synthase (iNOS) of goat macrophage cultures derived from monocytes (MDMs) infected for 4 and 24 h with Brucella melitensis strain 16 M. TNFα, IL-1β and iNOS, and IL-12p40, IFNγ and also iNOS were significantly expressed (p < 0.05) at 4 and 24 h respectively, in infected compared to non-infected MDMs. Therefore, the in vitro challenge of goat MDMs with B. melitensis promoted a transcriptional profile consistent with a type 1 response. However, when the immune response to B. melitensis infection was contrasted between MDM cultures phenotypically restrictive or permissive to intracellular multiplication of B. melitensis 16 M, it was observed that the relative IL-4 mRNA expression was significantly higher in permissive macrophage cultures with respect to restrictive cultures (p < 0.05), independently of the time p.i. A similar trend, although non-statistical, was recorded for IL-10, but not for pro-inflammatory cytokines. Thus, the up-expression profile of inhibitory instead of pro-inflammatory cytokines could explain, in part, the difference observed in the ability to restrict intracellular replication of Brucella. In this sense, the present results make a significant contribution to the knowledge of the immune response induced by B. melitensis in macrophages of its preferential host species. Instituto de Patobiología Fil: Maurizio, Estefania. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina. Fil: Maurizio, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rossi, Ursula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Rossi, Ursula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Trangoni, Marcos David. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Argentina Fil: Trangoni, Marcos David. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rossetti, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina. Fil: Rossetti, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2023-04-13T16:45:53Z 2023-04-13T16:45:53Z 2023-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/14473 https://www.sciencedirect.com/science/article/pii/S0171298523000438 0171-2985 1878-3279 https://doi.org/10.1016/j.imbio.2023.152375 eng info:eu-repograntAgreement/INTA/PNSA-1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional. info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Immunobiology 228 (3) : 152375 (May 2023) |
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Brucella melitensis Citoquinas Genética Caprinos Respuesta Inmunológica Brucelosis Macrofagos Cytokines Genetics Goats Immune Response Brucellosis Macrophages Brucella melitensis Citoquinas Genética Caprinos Respuesta Inmunológica Brucelosis Macrofagos Cytokines Genetics Goats Immune Response Brucellosis Macrophages |
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Brucella melitensis Citoquinas Genética Caprinos Respuesta Inmunológica Brucelosis Macrofagos Cytokines Genetics Goats Immune Response Brucellosis Macrophages Brucella melitensis Citoquinas Genética Caprinos Respuesta Inmunológica Brucelosis Macrofagos Cytokines Genetics Goats Immune Response Brucellosis Macrophages Maurizio, Estefanía Rossi, Ursula Amaranta Trangoni, Marcos David Rossetti, Carlos Alberto Cytokine expression profile of B. melitensis-infected goat monocyte-derived macrophages |
description |
Brucella parasitize the macrophage where is able to replicate and modulate the immune response in order to establish a chronic infection. The most adequate response to control and eliminate Brucella infection is a type 1 (Th1) cell-mediated effector immunity. Research in immune response of B. melitensis-infected goats is relatively scarce. In this study, we first evaluated changes in the gene expression of cytokines, a chemokine (CCL2) and the inducible nitric oxide synthase (iNOS) of goat macrophage cultures derived from monocytes (MDMs) infected for 4 and 24 h with Brucella melitensis strain 16 M. TNFα, IL-1β and iNOS, and IL-12p40, IFNγ and also iNOS were significantly expressed (p < 0.05) at 4 and 24 h respectively, in infected compared to non-infected MDMs. Therefore, the in vitro challenge of goat MDMs with B. melitensis promoted a transcriptional profile consistent with a type 1 response. However, when the immune response to B. melitensis infection was contrasted between MDM cultures phenotypically restrictive or permissive to intracellular multiplication of B. melitensis 16 M, it was observed that the relative IL-4 mRNA expression was significantly higher in permissive macrophage cultures with respect to restrictive cultures (p < 0.05), independently of the time p.i. A similar trend, although non-statistical, was recorded for IL-10, but not for pro-inflammatory cytokines. Thus, the up-expression profile of inhibitory instead of pro-inflammatory cytokines could explain, in part, the difference observed in the ability to restrict intracellular replication of Brucella. In this sense, the present results make a significant contribution to the knowledge of the immune response induced by B. melitensis in macrophages of its preferential host species. |
format |
info:ar-repo/semantics/artículo |
topic_facet |
Brucella melitensis Citoquinas Genética Caprinos Respuesta Inmunológica Brucelosis Macrofagos Cytokines Genetics Goats Immune Response Brucellosis Macrophages |
author |
Maurizio, Estefanía Rossi, Ursula Amaranta Trangoni, Marcos David Rossetti, Carlos Alberto |
author_facet |
Maurizio, Estefanía Rossi, Ursula Amaranta Trangoni, Marcos David Rossetti, Carlos Alberto |
author_sort |
Maurizio, Estefanía |
title |
Cytokine expression profile of B. melitensis-infected goat monocyte-derived macrophages |
title_short |
Cytokine expression profile of B. melitensis-infected goat monocyte-derived macrophages |
title_full |
Cytokine expression profile of B. melitensis-infected goat monocyte-derived macrophages |
title_fullStr |
Cytokine expression profile of B. melitensis-infected goat monocyte-derived macrophages |
title_full_unstemmed |
Cytokine expression profile of B. melitensis-infected goat monocyte-derived macrophages |
title_sort |
cytokine expression profile of b. melitensis-infected goat monocyte-derived macrophages |
publisher |
Elsevier |
publishDate |
2023-05 |
url |
http://hdl.handle.net/20.500.12123/14473 https://www.sciencedirect.com/science/article/pii/S0171298523000438 https://doi.org/10.1016/j.imbio.2023.152375 |
work_keys_str_mv |
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_version_ |
1798158118300942336 |