Molecular detection and phylogenetic analysis of Hepatozoon spp. in questing Ixodes ricinus ticks and rodents from Slovakia and Czech Republic
By amplification and sequencing of 18S rRNA gene fragments, Hepatozoon spp. DNA was detected in 0.08 % (4/5057) and 0.04 % (1/2473) of questing Ixodes ricinus ticks from Slovakia and Czech Republic, respectively. Hepatozoon spp. DNA was also detected in spleen and/or lungs of 4.45 % (27/606) of rodents from Slovakia. Prevalence of infection was significantly higher in Myodes glareolus (11.45 %) than in Apodemus spp. (0.28 %) (P < 0.001). Sequencing of 18S rRNA Hepatozoon spp. gene amplicons from I. ricinus showed 100 % identity with Hepatozoon canis isolates from red foxes or dogs in Europe. Phylogenetic analysis showed that at least two H. canis 18S rRNA genotypes exist in Slovakia of which one was identified also in the Czech Republic. The finding of H. canis in questing I. ricinus suggests the geographical spread of the parasite and a potential role of other ticks as its vectors in areas where Rhipicephalus sanguineus is not endemic. Sequencing of 18S rRNA gene amplicons from M. glareolus revealed the presence of two closely related genetic variants, Hepatozoon sp. SK1 and Hepatozoon sp. SK2, showing 99–100 % identity with isolates from M. glareolus from other European countries. Phylogenetic analysis demonstrates that 18S rRNA variants SK1 and SK2 correspond to previously described genotypes UR1 and UR2 of H. erhardovae, respectively. The isolate from Apodemus flavicollis (Hepatozoon sp. SK3b) was 99 % identical with isolates from reptiles in Africa and Asia. Further studies are necessary to identify the taxonomic status of Hepatozoon spp. parasitizing rodents in Europe and the host-parasite interactions in natural foci.
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Format: | info:ar-repo/semantics/artículo biblioteca |
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2016-10
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Subjects: | Ixodes Ricinus, Metastigmata, Hepatozoon, Sporozoa, Roedores, Rodents, Garrapatas, Apicomplexa, Eslovaquia, República Checa, |
Online Access: | http://hdl.handle.net/20.500.12123/1347 https://link.springer.com/article/10.1007%2Fs00436-016-5156-5 https://doi.org/10.1007/s00436-016-5156-5 |
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Ixodes Ricinus Metastigmata Hepatozoon Sporozoa Roedores Rodents Garrapatas Apicomplexa Eslovaquia República Checa Ixodes Ricinus Metastigmata Hepatozoon Sporozoa Roedores Rodents Garrapatas Apicomplexa Eslovaquia República Checa |
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Ixodes Ricinus Metastigmata Hepatozoon Sporozoa Roedores Rodents Garrapatas Apicomplexa Eslovaquia República Checa Ixodes Ricinus Metastigmata Hepatozoon Sporozoa Roedores Rodents Garrapatas Apicomplexa Eslovaquia República Checa Hamšíkova, Zuzana Silaghi, Cornelia Rudolf, Ivo Venclíková, Kristýna Mahríkova, Lenka Slovák, Mirko Mendel, Jan Blažejová, Hana Berthová, Lenka Kocianová, Elena Hubálek, Zdeněk Schnittger, Leonhard Kazimírová, Mária Molecular detection and phylogenetic analysis of Hepatozoon spp. in questing Ixodes ricinus ticks and rodents from Slovakia and Czech Republic |
description |
By amplification and sequencing of 18S rRNA gene fragments, Hepatozoon spp. DNA was detected in 0.08 % (4/5057) and 0.04 % (1/2473) of questing Ixodes ricinus ticks from Slovakia and Czech Republic, respectively. Hepatozoon spp. DNA was also detected in spleen and/or lungs of 4.45 % (27/606) of rodents from Slovakia. Prevalence of infection was significantly higher in Myodes glareolus (11.45 %) than in Apodemus spp. (0.28 %) (P < 0.001). Sequencing of 18S rRNA Hepatozoon spp. gene amplicons from I. ricinus showed 100 % identity with Hepatozoon canis isolates from red foxes or dogs in Europe. Phylogenetic analysis showed that at least two H. canis 18S rRNA genotypes exist in Slovakia of which one was identified also in the Czech Republic. The finding of H. canis in questing I. ricinus suggests the geographical spread of the parasite and a potential role of other ticks as its vectors in areas where Rhipicephalus sanguineus is not endemic. Sequencing of 18S rRNA gene amplicons from M. glareolus revealed the presence of two closely related genetic variants, Hepatozoon sp. SK1 and Hepatozoon sp. SK2, showing 99–100 % identity with isolates from M. glareolus from other European countries. Phylogenetic analysis demonstrates that 18S rRNA variants SK1 and SK2 correspond to previously described genotypes UR1 and UR2 of H. erhardovae, respectively. The isolate from Apodemus flavicollis (Hepatozoon sp. SK3b) was 99 % identical with isolates from reptiles in Africa and Asia. Further studies are necessary to identify the taxonomic status of Hepatozoon spp. parasitizing rodents in Europe and the host-parasite interactions in natural foci. |
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info:ar-repo/semantics/artículo |
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Ixodes Ricinus Metastigmata Hepatozoon Sporozoa Roedores Rodents Garrapatas Apicomplexa Eslovaquia República Checa |
author |
Hamšíkova, Zuzana Silaghi, Cornelia Rudolf, Ivo Venclíková, Kristýna Mahríkova, Lenka Slovák, Mirko Mendel, Jan Blažejová, Hana Berthová, Lenka Kocianová, Elena Hubálek, Zdeněk Schnittger, Leonhard Kazimírová, Mária |
author_facet |
Hamšíkova, Zuzana Silaghi, Cornelia Rudolf, Ivo Venclíková, Kristýna Mahríkova, Lenka Slovák, Mirko Mendel, Jan Blažejová, Hana Berthová, Lenka Kocianová, Elena Hubálek, Zdeněk Schnittger, Leonhard Kazimírová, Mária |
author_sort |
Hamšíkova, Zuzana |
title |
Molecular detection and phylogenetic analysis of Hepatozoon spp. in questing Ixodes ricinus ticks and rodents from Slovakia and Czech Republic |
title_short |
Molecular detection and phylogenetic analysis of Hepatozoon spp. in questing Ixodes ricinus ticks and rodents from Slovakia and Czech Republic |
title_full |
Molecular detection and phylogenetic analysis of Hepatozoon spp. in questing Ixodes ricinus ticks and rodents from Slovakia and Czech Republic |
title_fullStr |
Molecular detection and phylogenetic analysis of Hepatozoon spp. in questing Ixodes ricinus ticks and rodents from Slovakia and Czech Republic |
title_full_unstemmed |
Molecular detection and phylogenetic analysis of Hepatozoon spp. in questing Ixodes ricinus ticks and rodents from Slovakia and Czech Republic |
title_sort |
molecular detection and phylogenetic analysis of hepatozoon spp. in questing ixodes ricinus ticks and rodents from slovakia and czech republic |
publishDate |
2016-10 |
url |
http://hdl.handle.net/20.500.12123/1347 https://link.springer.com/article/10.1007%2Fs00436-016-5156-5 https://doi.org/10.1007/s00436-016-5156-5 |
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oai:localhost:20.500.12123-13472019-02-01T18:39:43Z Molecular detection and phylogenetic analysis of Hepatozoon spp. in questing Ixodes ricinus ticks and rodents from Slovakia and Czech Republic Hamšíkova, Zuzana Silaghi, Cornelia Rudolf, Ivo Venclíková, Kristýna Mahríkova, Lenka Slovák, Mirko Mendel, Jan Blažejová, Hana Berthová, Lenka Kocianová, Elena Hubálek, Zdeněk Schnittger, Leonhard Kazimírová, Mária Ixodes Ricinus Metastigmata Hepatozoon Sporozoa Roedores Rodents Garrapatas Apicomplexa Eslovaquia República Checa By amplification and sequencing of 18S rRNA gene fragments, Hepatozoon spp. DNA was detected in 0.08 % (4/5057) and 0.04 % (1/2473) of questing Ixodes ricinus ticks from Slovakia and Czech Republic, respectively. Hepatozoon spp. DNA was also detected in spleen and/or lungs of 4.45 % (27/606) of rodents from Slovakia. Prevalence of infection was significantly higher in Myodes glareolus (11.45 %) than in Apodemus spp. (0.28 %) (P < 0.001). Sequencing of 18S rRNA Hepatozoon spp. gene amplicons from I. ricinus showed 100 % identity with Hepatozoon canis isolates from red foxes or dogs in Europe. Phylogenetic analysis showed that at least two H. canis 18S rRNA genotypes exist in Slovakia of which one was identified also in the Czech Republic. The finding of H. canis in questing I. ricinus suggests the geographical spread of the parasite and a potential role of other ticks as its vectors in areas where Rhipicephalus sanguineus is not endemic. Sequencing of 18S rRNA gene amplicons from M. glareolus revealed the presence of two closely related genetic variants, Hepatozoon sp. SK1 and Hepatozoon sp. SK2, showing 99–100 % identity with isolates from M. glareolus from other European countries. Phylogenetic analysis demonstrates that 18S rRNA variants SK1 and SK2 correspond to previously described genotypes UR1 and UR2 of H. erhardovae, respectively. The isolate from Apodemus flavicollis (Hepatozoon sp. SK3b) was 99 % identical with isolates from reptiles in Africa and Asia. Further studies are necessary to identify the taxonomic status of Hepatozoon spp. parasitizing rodents in Europe and the host-parasite interactions in natural foci. Fil: Hamšíková, Zuzana. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia Fil: Silaghi, Cornelia. Ludwig-Maximilians-Universität. Comparative Tropical Medicine and Parasitology; Alemania. University of Zurich. Institute of Parasitology. National Centre for Vector Entomology; Suiza Fil: Rudolf, Ivo. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa Fil: Venclíková, Kristýna. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa. The Czech Academy of Sciences. Institute of Macromolecular Chemistry; República Checa Fil: Mahrikova, Lenka. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia Fil: Slovak, Mirko. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia Fil: Mendel, Jan. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa. Fil: Blažejová, Hana. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa. Fil: Berthova, Lenka. Slovak Academy of Sciences. Biomedical Research Center. Institute of Virology; Eslovaquia Fil: Kocianova, Elena. Slovak Academy of Sciences. Biomedical Research Center. Institute of Virology; Eslovaquia Fil: Hubálek, Zdeněk. The Czech Academy of Sciences. Institute of Vertebrate Biology; República Checa Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Kazimirová, Mária. Slovak Academy of Sciences. Institute of Zoology; Eslovaquia 2017-09-28T13:48:52Z 2017-09-28T13:48:52Z 2016-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1347 https://link.springer.com/article/10.1007%2Fs00436-016-5156-5 0932-0113 (Print) 1432-1955 (Online) https://doi.org/10.1007/s00436-016-5156-5 eng info:eu-repo/semantics/restrictedAccess application/pdf Slovakia (nation) Czech Republic (nation) Parasitology research 115 (10) : 3897–3904. (October 2016) |