Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species

Aim of study: The aim of the study was to characterize functional microsatellite markers in Prosopis alba and examine the transferability to species from the Prosopis genus. Area of the study: samples were obtained from natural populations of Argentina. Material and Methods: Eleven SSR functional markers related to stress and metabolism were amplified in a sample of 152 genotypes from P. alba, P. denudans, P. hassleri P. chilensis, P. flexuosa, and interspecific hybrids. Main results: In P. alba, the PIC average value was 0.36; and 6 out of the 11 primers showed high values of polymorphism ranging from 0.40 to 0.71. The cross-species transferability was high with high percentages of polymorphic loci. Research highlights: The SSR markers developed in P.alba were easily transferred to other Prosopis species which did not have functional markers.

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Main Authors: Pomponio, Maria Florencia, Acuña, Cintia Vanesa, Petreath, Vivien, Lopez Lauenstein, Diego, Marcucci Poltri, Susana Noemi, Torales, Susana Leonor
Format: info:ar-repo/semantics/artículo biblioteca
Language:eng
Published: Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España) 2015
Subjects:Prosopis alba, Marcadores Genéticos, Prosopis, Variación Genética, Microsatélites, Genetic Markers, Genetic Variation, Microsatellites, Marcadores SSR, SSR Markers,
Online Access:http://hdl.handle.net/20.500.12123/11356
https://revistas.inia.es/index.php/fs/article/view/7188
https://doi.org/10.5424/fs/2015242-07188
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spelling oai:localhost:20.500.12123-113562022-03-09T16:19:35Z Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species Pomponio, Maria Florencia Acuña, Cintia Vanesa Petreath, Vivien Lopez Lauenstein, Diego Marcucci Poltri, Susana Noemi Torales, Susana Leonor Prosopis alba Marcadores Genéticos Prosopis Variación Genética Microsatélites Genetic Markers Genetic Variation Microsatellites Marcadores SSR SSR Markers Aim of study: The aim of the study was to characterize functional microsatellite markers in Prosopis alba and examine the transferability to species from the Prosopis genus. Area of the study: samples were obtained from natural populations of Argentina. Material and Methods: Eleven SSR functional markers related to stress and metabolism were amplified in a sample of 152 genotypes from P. alba, P. denudans, P. hassleri P. chilensis, P. flexuosa, and interspecific hybrids. Main results: In P. alba, the PIC average value was 0.36; and 6 out of the 11 primers showed high values of polymorphism ranging from 0.40 to 0.71. The cross-species transferability was high with high percentages of polymorphic loci. Research highlights: The SSR markers developed in P.alba were easily transferred to other Prosopis species which did not have functional markers. Instituto de Recursos Biológicos Fil: Pomponio, Maria Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Acuña, Cintia Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Petreath, Vivien. Universidad Nacional de Patagonia San Juan Bosco. Sede Comodoro Rivadavia; Argentina Fil: Lopez Lauenstein, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Marcucci Poltri, Susana Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Torales, Susana Leonor. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina 2022-03-09T16:16:24Z 2022-03-09T16:16:24Z 2015 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11356 https://revistas.inia.es/index.php/fs/article/view/7188 2171-9845 2171-5068 https://doi.org/10.5424/fs/2015242-07188 eng info:eu-repograntAgreement/INTA/PNBIO-1131044/AR./Genómica aplicada a estudios de ecología molecular y diversidad genética. info:eu-repo/semantics/openAccess application/pdf Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España) Forest Systems 24 (2) : eRC04 (2015)
institution INTA AR
collection DSpace
country Argentina
countrycode AR
component Bibliográfico
access En linea
databasecode dig-inta-ar
tag biblioteca
region America del Sur
libraryname Biblioteca Central del INTA Argentina
language eng
topic Prosopis alba
Marcadores Genéticos
Prosopis
Variación Genética
Microsatélites
Genetic Markers
Genetic Variation
Microsatellites
Marcadores SSR
SSR Markers
Prosopis alba
Marcadores Genéticos
Prosopis
Variación Genética
Microsatélites
Genetic Markers
Genetic Variation
Microsatellites
Marcadores SSR
SSR Markers
spellingShingle Prosopis alba
Marcadores Genéticos
Prosopis
Variación Genética
Microsatélites
Genetic Markers
Genetic Variation
Microsatellites
Marcadores SSR
SSR Markers
Prosopis alba
Marcadores Genéticos
Prosopis
Variación Genética
Microsatélites
Genetic Markers
Genetic Variation
Microsatellites
Marcadores SSR
SSR Markers
Pomponio, Maria Florencia
Acuña, Cintia Vanesa
Petreath, Vivien
Lopez Lauenstein, Diego
Marcucci Poltri, Susana Noemi
Torales, Susana Leonor
Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species
description Aim of study: The aim of the study was to characterize functional microsatellite markers in Prosopis alba and examine the transferability to species from the Prosopis genus. Area of the study: samples were obtained from natural populations of Argentina. Material and Methods: Eleven SSR functional markers related to stress and metabolism were amplified in a sample of 152 genotypes from P. alba, P. denudans, P. hassleri P. chilensis, P. flexuosa, and interspecific hybrids. Main results: In P. alba, the PIC average value was 0.36; and 6 out of the 11 primers showed high values of polymorphism ranging from 0.40 to 0.71. The cross-species transferability was high with high percentages of polymorphic loci. Research highlights: The SSR markers developed in P.alba were easily transferred to other Prosopis species which did not have functional markers.
format info:ar-repo/semantics/artículo
topic_facet Prosopis alba
Marcadores Genéticos
Prosopis
Variación Genética
Microsatélites
Genetic Markers
Genetic Variation
Microsatellites
Marcadores SSR
SSR Markers
author Pomponio, Maria Florencia
Acuña, Cintia Vanesa
Petreath, Vivien
Lopez Lauenstein, Diego
Marcucci Poltri, Susana Noemi
Torales, Susana Leonor
author_facet Pomponio, Maria Florencia
Acuña, Cintia Vanesa
Petreath, Vivien
Lopez Lauenstein, Diego
Marcucci Poltri, Susana Noemi
Torales, Susana Leonor
author_sort Pomponio, Maria Florencia
title Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species
title_short Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species
title_full Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species
title_fullStr Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species
title_full_unstemmed Characterization of functional SSR markers in Prosopis alba and their transferability across Prosopis species
title_sort characterization of functional ssr markers in prosopis alba and their transferability across prosopis species
publisher Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España)
publishDate 2015
url http://hdl.handle.net/20.500.12123/11356
https://revistas.inia.es/index.php/fs/article/view/7188
https://doi.org/10.5424/fs/2015242-07188
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